Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression
- 1John Innes Centre, Norwich NR4 7UH, United Kingdom;
- 2Institute of Biochemistry and Biology, University of Potsdam, DE-14476 Potsdam-Golm, Germany;
- 3Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
- Corresponding author: caroline.dean{at}jic.ac.uk
Abstract
Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNA-binding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression.
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Footnotes
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Supplemental material is available for this article.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.305227.117.
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Freely available online through the Genes & Development Open Access option.
- Received August 10, 2017.
- Accepted November 7, 2017.
This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
