Genomic imprinting of Xist by maternal H3K27me3
- 1Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA;
- 2Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, USA;
- 3Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Boston, Massachusetts 02115, USA;
- 4Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA;
- 5Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA
- Corresponding authors: yzhang{at}genetics.med.harvard.edu, azusa.inoue{at}riken.jp
Abstract
Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte growth and persists through preimplantation development in mice. Loss of maternal H3K27me3 induces maternal Xist expression and maternal XCI in preimplantation embryos. Our study thus identifies maternal H3K27me3 as the imprinting mark of Xist.
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Footnotes
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Supplemental material is available for this article.
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.304113.117.
- Received July 1, 2017.
- Accepted September 28, 2017.
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