Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C
Abstract
Chromatin assembly and DNA replication are temporally coupled, and DNA replication in the absence of histone synthesis causes inviability. Here we demonstrate that chromatin assembly factor Asf1 also affects DNA replication. In budding yeast cells lacking Asf1, the amounts of several DNA replication proteins, including replication factor C (RFC), proliferating cell nuclear antigen (PCNA), and DNA polymerase ϵ (Pol ϵ), are reduced at stalled replication forks. In contrast, DNA polymerase α (Pol α) accumulates to higher than normal levels at stalled forks in asf1Δ cells. Using purified, recombinant proteins, we demonstrate that RFC directly binds Asf1 and can recruit Asf1 to DNA molecules in vitro. We conclude that histone chaperone protein Asf1 maintains a subset of replication elongation factors at stalled replication forks and directly interacts with the replication machinery.
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Footnotes
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Supplemental material is available at http://www.genesdev.org.
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Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1305005.
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↵3 Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
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↵4 Address as of July 1, 2005: Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA.
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↵5 Corresponding author. E-MAIL pdkaufman{at}lbl.gov; FAX (510) 486-6488.
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- Accepted April 12, 2005.
- Received February 11, 2005.
- Cold Spring Harbor Laboratory Press
