The Something About Silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAFII30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)–FACT complex

doi:10.1101/gad.14.10.1196

The Something About Silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF_II30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)–FACT complex

¹Howard Hughes Medical Institute (HHMI), ²Department of Biochemistry and Molecular Biology, Penn State University, University Park, Pennsylvania, 16802-4500 USA; ³Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794 USA

Abstract

We have purified and characterized a Gcn5-independent nucleosomal histone H3 HAT complex, NuA3 (NucleosomalAcetyltransferase of histone H3). Peptide sequencing of proteins from the purified NuA3 complex identified Sas3 as the catalytic HAT subunit of the complex. Sas3 is the yeast homolog of the human MOZ oncogene. Sas3 is required for both the HAT activity and the integrity of the NuA3 complex. In addition, NuA3 contains the TBP- associated factor, yTAF_II30, which is also a component of the TFIID, TFIIF, and SWI/SNF complexes. Sas3 mediates interaction of the NuA3 complex with Spt16 both in vivo and in vitro. Spt16 functions as a component of the yeast CP (Cdc68/Pob3) and mammalian FACT (facilitateschromatin transcription) complexes, which are involved in transcription elongation and DNA replication. This interaction suggests that the NuA3 complex might function in concert with FACT–CP to stimulate transcription or replication elongation through nucleosomes by providing a coupled acetyltransferase activity.