Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint

  1. Andrew Bloecher and
  2. Kelly Tatchell
  1. Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport, Louisiana 71130 USA

Abstract

A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mitosis. Loss of the spindle/kinetochore checkpoint inglc7-129 cells abolishes the G2/M cell cycle arrest with a concomitant increase in chromosome loss and reduced viability. These results support a role for Glc7p in regulating kinetochore attachment to the spindle, an event monitored by the spindle/kinetochore checkpoint.

Keywords

Footnotes

  • ↵Corresponding author.

  • E-MAIL ktatch{at}mail.sh.lsumc.edu; FAX (318) 675-5180.

    • Received October 13, 1998.
    • Accepted January 13, 1999.
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