GAGA mediates the enhancer blocking activity of the eve promoter in the Drosophila embryo
Abstract
Insulator DNAs and promoter competition regulate enhancer–promoter interactions within complex genetic loci. A transgenic embryo assay was used to obtain evidence that theDrosophila eve promoter possesses an insulator activity that can be uncoupled from the core elements that mediate competition. Theeve promoter contains an optimal TATA element and a GAGA sequence. The analysis of various chimeric promoters provides evidence that TATA is essential for promoter competition, whereas GAGA mediates enhancer blocking. The Trithorax-like (Trl) protein interacts with GAGA, and mutations in trl attenuate eve promoter insulator activity. We suggest that Trl–GAGA increases the stability of enhancer–promoter interactions by creating an open chromatin configuration at the core promoter.
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Footnotes
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↵1 Corresponding author.
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E-MAIL mlevine{at}uclink4.berkeley.edu; FAX (510) 643-5785.
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- Received July 28, 1998.
- Accepted September 9, 1998.
- Cold Spring Harbor Laboratory Press
