Errata for vol. 19, p. 2307
Genes & Development 19: 2307-2319 (2005)
The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones
Baris Tursun, Anne Schlüter, Marvin A. Peters, Birte Viehweger, Heather P. Ostendorff, Juliana Soosairajah, Alexander Drung, Michael Bossenz, Steven A. Johnsen, Michaela Schweizer, Ora Bernard, and Ingolf Bach
In the above-mentioned paper, there was an error in the reproduction of Figure 6E in which one panel was mistakenly duplicated. The correct figure appears below, with its legend.
Proteasomal regulation of LIMK1 in growth cones. (A) Proteasomal regulation of LIMK1 levels in cultures of dissociated hippocampal neurons. Western blot analysis of total protein extracts of primary hippocampal neuron cultures treated with the proteasome inhibitor MG132 for 1, 3, and 6 h, probed with anti-LIMK1 and β-tubulin antibodies. (B) LIMK1 concentrations at the growth cones are regulated by the proteasome. Primary hippocampal neurons cultured for 2 d were treated for 1, 3, and 6 h with MG132, followed by staining with anti-LIMK1 antibodies (green). Only neurofilament-positive projections are shown. The time-dependent increase in LIMK1 concentrations was so high that the photomultiplier of the confocal microscope was set to a 50% lower level for cells treated for 3 and 6 h with MG132. Representative cells from three independent experiments are shown. (C) Relative LIMK1 levels in axonal growth cones of hippocampal neurons after 6 h of MG132 treatment as measured by fluorescence intensity of same-sized growth cone areas in three independent sets of experiments. Relative fluorescence intensities at 0 h of MG132 treatment were measured and normalized as 1. The total number of growth cones measured is indicated. (⋆⋆) P < 0.0001. (D) The mouse UbcH5 ortholog is expressed in growth cones. Primary hippocampal neurons cultured for 2 d were stained with anti-UbcH5 antibodies (red). A neurofilament-positive projection is shown. (Right panel) A phase-contrast image of the same neuron. (E) Treatment with proteasome inhibitor results in colocalization of Rnf6 and LIMK1 over large parts of the growth cone. Primary hippocampal neuron cultured for 2 d and treated with MG132 for 3 h were stained for Rnf6 (red) and LIMK1 (green). Only neurofilament-positive projections are shown. Bar, 4 μm. Images were captured by confocal microscopy using a 63× objective. Enlargement of the boxed region is shown in the right panel. Note that colocalization of Rnf6 and LIMK1 occurs over large parts of the growth cone. Similar results were obtained in five independent experiments.
