QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) Supplemental Reearch Data All Versions of this Article: gad.1561307v1 21/13/1598    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geldner, N. Articles by Chory, J. Search for Related Content PubMed PubMed Citation Articles by Geldner, N. Articles by Chory, J. Related Content Related Article Social Bookmarking                   What's this?

RESEARCH COMMUNICATION

Endosomal signaling of plant steroid receptor kinase BRI1

¹ Plant Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA; ² Howard Hughes Medical Institute Plant Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA; ³ Zentrum für Molekularbiologie der Pflanzen, 72076 Tübingen, Germany

	Abstract

Top Abstract Results and Discussion Materials and methods Acknowledgments References

 
The LRR receptor serine/threonine kinases are a major eukaryotic receptor family, for which the central regulatory mechanism of endosomal trafficking remains largely unadressed. We show that the steroid receptor BRI1 localizes to both plasma membrane and early endosomal compartments, even when observed at low, endogenous expression levels, and that its localization and turnover are independent of ligand. However, increasing endosomal localization of BRI1 enhances activation of the pathway and genomic responses. Our data indicate distinct signaling and trafficking mechanisms within this receptor class and show that the use of endosomes as signaling compartments is an unexpectedly broad phenomenon in eukaryotes.

[Keywords: Brassinosteroid; endosomes; plant receptor kinase; Arabidopsis; receptor trafficking]

Received April 12, 2007; revised version accepted May 16, 2007.

As the other big branch of multicellular life, plants have independently evolved a different set of receptors and show considerable divergence in endosomal structures and trafficking components (Shiu and Bleecker 2001; Geldner and Jurgens 2006). The >200 LRR receptor Ser–Thr kinases (unrelated to TGF- receptors) represent the predominant receptor family in the plant kingdom. Although the vast majority of them are orphan receptors (Morillo and Tax 2006), the steroid receptor BRI1 has been intensively studied and provides a good model for plant receptor kinase activation (Vert et al. 2005).

BRI1 is the receptor for brassinosteroids (BRs), which are crucial growth-promoting hormones in plants. Knock-out of BRI1 leads to extremely dwarfed and entirely BR-insensitive plants (Clouse et al. 1996; Li and Chory 1997; Kinoshita et al. 2005). Binding of brassinolide (BL), the most active BR, to BRI1’s extracellular domain activates the BRI1 kinase, releases the inhibitory BKI1 protein from the plasma membrane and increases it affinity for BAK1, a second LRR kinase (Wang et al. 2001, 2005; Nam and Li 2002; Wang and Chory 2006). This leads to dephosphorylation and consequent dimerization and DNA binding of the nuclear-localized BES1/BZR1 transcription factors, which control the genomic BR response (Yin et al. 2005; Vert and Chory 2006). This dephosphorylation is thought to be caused by a combination of inactivation of the GSK3/Shaggy kinase BIN2 (Vert and Chory 2006) and the phosphatase activity of BSU1 (Mora-Garcia et al. 2004). It is currently unknown how receptor activation leads to BIN2 inactivation, and the immediate relevant downstream targets of the activated receptor complex remain to be identified (Belkhadir and Chory 2006). Nonetheless, the known components provide a straightforward and specific read-out of receptor activity.

BRI1 has been shown to localize to the plasma membrane and endosomal structures, and co-overexpression of BRI1 together with the BAK1 coreceptor in protoplasts leads to increased internal accumulation of BRI1. Based on this, it was proposed that BAK1 regulates BRI1 trafficking (Russinova et al. 2004). However, an effect of BAK1 on BRI1 localization could not be demonstrated in intact plants, and the inherent limitations of the protoplast system precluded attempts to functionally connect endocytosis to signaling of BRI1.

	Results and Discussion

Top Abstract Results and Discussion Materials and methods Acknowledgments References

 
BRI1 is expressed and required in root meristems, a stem cell tissue that is accessible to live imaging without dissection. This provides the rare opportunity to address the connection between receptor localization and signaling in an intact organ. Previously, BRI1 localization to endosomal compartments has been observed exclusively in a BRI1-GFP-overexpressing line that shows phenotypes of enhanced BR response (Fig. 1A), displaying increased root elongation in the absence of ligand, indicative of inappropriate, partially constitutive receptor activity (Fig. 1B). Since we were concerned that the observed endosomal localization was an artifact of overexpression, we constructed a new GFP fusion and selected a line with endogenous expression levels (Fig. 1C). We ascertained that this line had no overexpression phenotypes and rescued a null mutant (Fig. 1A,B; Supplementary Table 1). We detected a fairly weak but specific GFP signal in root meristem cells (Fig. 1D,E; Supplementary Fig. 1), both in the plasma membrane and highly mobile, pleiomorphic intracellular structures (Supplementary Movie 1). These BRI1-positive structures are at least in part early endosomes because they colocalize with the endocytic tracer FM4-64 after 5–10 min of uptake (Fig. 1F). Additionally, they display partial colocalization with VHA-a1-RFP (Fig. 1G), a marker for TGN/early endosomes in plants (Dettmer et al. 2006).

View larger version (112K): [in this window] [in a new window]   Figure 1. Endogenously expressed BRI1-GFP localizes to endosomes. (A) Representative pictures of rosette stage Arabidopsis grown under identical conditions. The BRI1-GFP line expressing at endogenous levels (endog.) is indistinguishable from wild type, whereas the overexpressing line (ox.) shows the reported overexpression phenotypes of narrow leaf blades and elongated, twisting petioles (leaf stalks). (B) Roots were depleted of endogenous BRs by growth on 5 µM brassinazole for 3 d. Primary root growth was assessed after three more days (n = 10 per line). Percent growth relative to untreated control is shown. The line expressing BRI1-GFP at endogenous levels is inhibited to wild-type levels, whereas the overexpressing line (ox.) shows less inhibition of root growth in the absence of ligand. (C) Immunoblot of the same lines detected with -BRI1 antibodies. Intensity of the BRI1-GFP (top) band is much stronger than the band of endogenous BRI1 (bottom) in the overexpressing line, but both bands show the same intensity in middle lane. (D,E) Subcellular localization and levels of BRI1-GFP in root meristem epidermal cells. (D) BRI1-GFP-overexpressing line. (E) BRI1-GFP endogenous expresser. (F) BRI1-GFP (green) partially colocalizes with the endocytic tracer FM4-64 (red) after 5–10 min uptake. (G) BRI1-GFP (green) also colocalizes partially with VHA-a1-RFP (red). See Supplementary Figure 1 for individual channels of overlays. Bars, 10 µm.

View larger version (105K): [in this window] [in a new window]   Figure 2. Ligand-independent localization and turnover of BRI1. (A) Seedling roots were treated with saturating amounts of ligand (BL, 1 µM), after recording of first frame (0 min) and were observed at 1-min intervals. BRI1-GFP localization remains unaltered. (B) As in A, but roots were depleted of endogenous BR by growing on 5-µM brassinazole for 3 d. BRI1-GFP localization remains unchanged. (C) In vivo pulse-chase analysis of BRI1-YFP. Transgenic lines driving BRI1-YFP under a heat-shock-inducible promoter were used to measure the half-life of BRI1 in vivo and exclusively in root meristematic cells by quantitative confocal microscopy. YFP signal intensity peaked 4 h after heat shock, when mRNA levels were already back to basal levels (not shown). Turnover was recorded as the reduction in YFP intensity relative to peak expression. Data points were recorded every 2 min. Half-life of BRI1 was determined to be 5 h and this turnover rate appeared to be entirely independent of ligand, as much in untreated roots (left) as in ligand-depleted roots (right) (grown as in B). (Below) Picture row shows an example of signal recordings for five untreated control root meristems. (D, left) BKI-YFP displays clear PM accumulation over cytosolic background, whereas no comparable signal can be observed in endosomal compartments. (Right) BRI1-GFP grown under identical conditions. Bars: A,B, 10 µm.

Activation of signaling pathways can lead to a relevant increase in protein flux between compartments, without necessarily shifting their steady-state accumulation in one or the other direction (Ando et al. 2004). In the case of BRI1, an increase in endocytosis and degradation could be offset by increased synthesis and secretion, without causing observable changes in overall localization or levels of BRI1-GFP. Therefore, we decided to directly measure BRI1 turnover rates in planta. To do so, we placed BRI1-YFP under the control of a heat-shock promoter, generating lines that allow pulsed expression of the tagged BRI1 by a 20–30-min induction at 37°C (Supplementary Movie 4). In combination with quantitative confocal microscopy, this allowed for a pulse-chase analysis exclusively in intact root meristems (Fig. 2C; Supplementary Fig. 2; Supplementary Movie 5). We determined the half-life of BRI1-YFP to be 5 h. This relative stability suggests that BRI1 either recycles during its lifetime or that only a minor fraction of BRI1 engages in endocytic transport to the vacuole with a majority remaining immobile. Again, the measured degradation rate of BRI1 was unaffected by addition of BL either to untreated or ligand-depleted roots (Fig. 2C). Based on these results, it is unlikely that BRs cause any difference in BRI1 intracellular transport, and we conclude that BRI1 trafficking is constitutive.

The activity status of BRI1 cannot be monitored directly at subcellular resolution. However, in untreated root cells a significant amount of plasma-membrane-localized BRI1 is apparently in an inactive state, as judged by the presence of the inhibitory BKI1 protein, which is released by BRI1-dependent phosphorylation after BR stimulation (Wang and Chory 2006). We did not observe any BKI1 accumulation in endosomal structures, suggesting that the endosomal BRI1 may represent an active receptor pool (Fig. 2D). To test this idea, we attempted to specifically manipulate the subcellular localization of BRI1 and to monitor the consequences on signaling activity. A number of known chemical inhibitors of trafficking were tested (data not shown), but only Brefeldin A (BFA) turned out to be sufficiently specific for our purpose. BFA is a widely used inhibitor of endosomal trafficking in plants (Geldner 2004). BFA had the expected effects on BRI1 localization, although we observed a fairly strong persistence of BRI1 signals at the plasma membrane. Nonetheless, we quantified plasma membrane versus intracellular signal in time-lapse images and showed that BFA induced a shift of BRI1 localization into endosomal compartments (Fig. 3A,B; Supplementary Movie 6). We also showed by pulse-chase analysis that BFA blocks translocation of BRI1 from earlier to late endosomal compartments and vacuoles, thereby interfering with BRI1-YFP degradation (Fig. 3C,D). This block in vacuolar transport is visualized, because BFA-treated cells did not accumulate residual YFP signals in vacuoles some hours after the expression pulse (Fig. 3D). Again, we did not observe any BKI1 accumulation in these BFA-induced endosomal aggregates, suggesting that the BRI1 therein could be part of active receptor complexes (Fig. 3E). For summary cartoon of BFA effects on BRI1 trafficking, see Supplementary Figure 3.

View larger version (98K): [in this window] [in a new window]   Figure 3. BFA increases endosomal localization of BRI1. (A) Time-lapse analysis of BFA-induced shift of BRI1 from the plasma membrane to endosomal compartments. (B) Quantification of time lapse in A. BFA induces a rapid drop in plasma membrane signal with a concomitant increase in intracellular fluorescence. A new steady state is apparently reached after 30 min. (C) Quantification of the effect of BFA on BRI1-YFP degradation in a pulse-chase experiment. BFA leads to a nearly complete block of BRI1 turnover. (D) BRI1-YFP localization in seedling root meristems 8 h after heat-shock-induced peak expression, with (left) or without (right) BFA treatment, taken under identical settings. BFA leads to strong accumulation of BRI1-YFP in endosomal aggregates, whereas only a weak and predominantly vacuolar BRI1-YFP signal is observed in the untreated control (arrowheads). (E) FM4-64 (red, left) and BKI1-YFP (green, middle) after 50 µM BFA treatment for 30 min. (Right) Overlay. Note the absence of BKI1 signal in BFA compartments. Bars: A,D,E, 10 µm.

View larger version (51K): [in this window] [in a new window]   Figure 4. BRI1 signaling is induced by BFA treatment. (A) A highly sensitive Arabidopsis green cell suspension line shows dephosphorylation of BES1 (green, above) and suppression of early-response gene, DWF4 (green, below) already at 1 nM BL for 30 min. (Middle) BFA treatment alone leads to an efficient activation of BES1 (red) and suppression early-response genes. External BL enhances the BFA effect on BES1 (yellow, above) and DWF4 (yellow, below). (B) Enhancement of BR signaling by BFA can also be observed in BRI1-overexpressing seedlings, when treating with low amounts of BL (10 nM) for 4 h, which leads to only half-maximal dephosporylation of BES1 (above) and a twofold reduction in DWF4 levels (green, below). Adding BFA (100 µM) after 30 min of BL pretreatment significantly enhances BES1 activation (above) and DWF4 suppression (red, below).

View larger version (43K): [in this window] [in a new window]   Figure 5. Induced BRI-YFP expression in cultured roots. (A) BFA effects can also be observed in intact organs using a bri1-null mutant root culture containing heat-shock-inducible BRI1-YFP. In this system, the effects of BFA on localization and signaling can be simultaneously assessed. Top panel shows BRI-YFP signals, 8 h after a 30-min heat-shock or control treatment. BFA treatment (100 µM) started 4 h after heat shock. Middle panel shows BRI1-YFP levels as detected on immunoblot with -BRI1 after same treatments as above. Bottom panel shows heat-shock-induced suppression of DWF4 (gray) and its further reduction by BFA treatment (red). Bar, 10 µm.

Together, this now provides us with two plant receptor models that display opposite trafficking behavior. Interestingly, this difference in trafficking matches an underlying difference in the biology of these two receptors. Whereas FLS2 is a pathogen receptor evolved for rare but acute signaling, BRI1 is thought to perceive a more or less continuous signal, whose relevance lies in its modulation more than in acute presence or absence.

A number of interesting speculations have been put forward to explain why endosomal signaling might have evolved in animals. It was proposed that signaling from numerous motile endosomes might overcome problems of diffusion-based signaling cascades in large cells and that timing and specificity of signaling events can be better controlled in endosomes (Miaczynska et al. 2004). Our data now indicate that endosomes in multicellular plants also act as signaling compartments, in spite of their independent sets of receptors and a differently organized endosomal system. We propose that a common, fundamental problem might have independently driven the development of signaling endosomes. Both multicellular plants and animals show vast increases of surface-localized receptor families. In such a situation, the available plasma membrane surface could become a limiting factor and trafficking of activated receptors to endosomes a means to increase the effective surface area available for signaling. This would restrict the dwelling time of receptors at the plasma membrane to ligand binding and activation, while compartmentalizing the longer-lasting downstream signaling events to the much less restricted inner surfaces of endosomal compartments.

	Materials and methods

Top Abstract Results and Discussion Materials and methods Acknowledgments References

 

Plant material and growth conditions

Arabidopsis Columbia seedlings were grown vertically for 4–6 d with 24 h light at 22°C on 0.5x LS (Linsmayer-Skoog plant growth mixture) agar plates. Green cell suspension cultures were originally obtained from Ruishuang Geng (Ohio State University, Columbus, OH) and grown as described by Kim et al. (2003). Suspension-grown root meristem cultures were obtained according to an online protocol at http://www.bio.net/bionet/mm/arab-gen/1992-September/000708.html.

Constructs and generation of transgenic lines

A BRI1-GFP construct was cloned into a pGREEN II vector derivative containing nos-promoter-driven Basta resistance. It contains 1689 base pairs (bp) upstream of the BRI1 translational start codon and 629 bp downstream from the stop codon. The EGFP sequence was inserted at the C terminus as described by Friedrichsen et al. (2000). The contruct was transformed into wild type, and a line with close to identical expression levels was selected. This line therefore still contains twice the amount of overall BRI1 product (endogenous BRI1 plus BRI1-GFP). The promoter of the HS::BRI1-YFP construct was described by Knox et al. (2003). EYFP was fused to the C terminus as above.

Confocal microscopy and image quantification

Confocal microscopy was done with a Leica SP/2 inverted microscope. Live imaging of roots was done by placing root tips on an agar block that fit into silicon chambers with a coverslip bottom (Grace Biolabs). Specimens were either sealed with a slide (long-term observation) or overlaid with sufficient liquid media (short-term observations). Image analysis was done with the Leica SP/2 software package and the ImageJ bundle provided by the Wright Cell Imaging facility.

Hormone and inhibitor treatments

BL was obtained from CIDtech Research, Inc., and Brefeldin A was obtained from Sigma. For long-term treatments, hormones or drugs were dissolved into agar blocks onto which roots were placed (see above). Short-term observations were done by mixing the hormone/drug into a liquid drop overlaying the agar-embedded root tips (see above) while continuously scanning.

Heat-shock pulse-chase analysis

For quantification of the BRI1 degradation rate, seedlings of homozygous HS::BRI1-YFP lines were transferred into in 200 µL of liquid 0.5x LS in a 2-mL cap and heat-shocked in a 37°C water bath for 20–30 min. Seedlings were then placed back onto an agar plate for 4.5 h, before being mounted for observation (see above). For initial experiments, entire agar plates with seedlings were sealed and left floating in the water bath for at least 45 min. Turnover rates were determined by measuring signal intensities in five regions of interest along the root meristem, as depicted in Supplementary Figure 2, of at least five roots per experiment.

	Acknowledgments

Top Abstract Results and Discussion Materials and methods Acknowledgments References

 
We thank Y. Belkhadir, M. Chen, and S. Salvadi-Goldstein for critical reading of the manuscript; Y. Yin for providing BES1 antibody; T. Dabi for maintenance of cell cultures; and F. Schöffl for the heat-shock promoter fragment. The work was supported by initial funding from the European Molecular Biology Organisation (EMBO) and continued funding from a Human Frontier Science Project (HFSP) long-term fellowship to N.G., by the Howard Hughes Medical Institute, and by USDA and NSF grants to J.C.

	Footnotes

 
⁴ Corresponding author.

E-MAIL chory{at}salk.edu; FAX (858) 558-6379.

Supplemental material is available at http://www.genesdev.org.

Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.1561307

	References

Top Abstract Results and Discussion Materials and methods Acknowledgments References

 
Ando, R., Mizuno, H., and Miyawaki, A. 2004. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306: 1370–1373.[Abstract/Free Full Text]

Baass, P.C., Di Guglielmo, G.M., Authier, F., Posner, B.I., and Bergeron, J.J. 1995. Compartmentalized signal transduction by receptor tyrosine kinases. Trends Cell Biol. 5: 465–470.[CrossRef][Medline]

Belkhadir, Y. and Chory, J. 2006. Brassinosteroid signaling: A paradigm for steroid hormone signaling from the cell surface. Science 314: 1410–1411.[Abstract/Free Full Text]

Clouse, S.D., Langford, M., and McMorris, T.C. 1996. A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development. Plant Physiol. 111: 671–678.[Abstract]

DeFea, K.A., Zalevsky, J., Thoma, M.S., Dery, O., Mullins, R.D., and Bunnett, N.W. 2000. -Arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J. Cell Biol. 148: 1267–1281.[Abstract/Free Full Text]

Dettmer, J., Hong-Hermesdorf, A., Stierhof, Y.D., and Schumacher, K. 2006. Vacuolar H+-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis. Plant Cell 18: 715–730.[Abstract/Free Full Text]

Friedrichsen, D.M., Joazeiro, C.A., Li, J., Hunter, T., and Chory, J. 2000. Brassinosteroid-insensitive-1 is a ubiquitously expressed leucine-rich repeat receptor serine/threonine kinase. Plant Physiol. 123: 1247–1256.[Abstract/Free Full Text]

Geldner, N. 2004. The plant endosomal system—Its structure and role in signal transduction and plant development. Planta 219: 547–560.[Medline]

Geldner, N. and Jurgens, G. 2006. Endocytosis in signalling and development. Curr. Opin. Plant Biol. 9: 589–594.[CrossRef][Medline]

Hicke, L., Zanolari, B., and Riezman, H. 1998. Cytoplasmic tail phosphorylation of the -factor receptor is required for its ubiquitination and internalization. J. Cell Biol. 141: 349–358.[Abstract/Free Full Text]

Kim, W.Y., Geng, R., and Somers, D.E. 2003. Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome. Proc. Natl. Acad. Sci. 100: 4933–4938.[Abstract/Free Full Text]

Kinoshita, T., Cano-Delgado, A., Seto, H., Hiranuma, S., Fujioka, S., Yoshida, S., and Chory, J. 2005. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 433: 167–171.[CrossRef][Medline]

Knox, K., Grierson, C.S., and Leyser, O. 2003. AXR3 and SHY2 interact to regulate root hair development. Development 130: 5769–5777.[Abstract/Free Full Text]

Li, J. and Chory, J. 1997. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90: 929–938.[CrossRef][Medline]

Miaczynska, M., Pelkmans, L., and Zerial, M. 2004. Not just a sink: Endosomes in control of signal transduction. Curr. Opin. Cell Biol. 16: 400–406.[CrossRef][Medline]

Mora-Garcia, S., Vert, G., Yin, Y., Cano-Delgado, A., Cheong, H., and Chory, J. 2004. Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis. Genes & Dev. 18: 448–460.[Abstract/Free Full Text]

Morillo, S.A. and Tax, F.E. 2006. Functional analysis of receptor-like kinases in monocots and dicots. Curr. Opin. Plant Biol. 9: 460–469.[CrossRef][Medline]

Nam, K.H. and Li, J. 2002. BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell 110: 203–212.[CrossRef][Medline]

Panopoulou, E., Gillooly, D.J., Wrana, J.L., Zerial, M., Stenmark, H., Murphy, C., and Fotsis, T. 2002. Early endosomal regulation of Smad dependent signaling in endothelial cells. J. Biol. Chem. 277: 18046–18052.[Abstract/Free Full Text]

Robatzek, S., Chinchilla, D., and Boller, T. 2006. Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis. Genes & Dev. 20: 537–542.[Abstract/Free Full Text]

Russinova, E., Borst, J.W., Kwaaitaal, M., Cano-Delgado, A., Yin, Y., Chory, J., and de Vries, S.C. 2004. Heterodimerization and endocytosis of Arabidopsis brassinosteroid receptors BRI1 and AtSERK3 (BAK1). Plant Cell 16: 3216–3229.[Abstract/Free Full Text]

Sekimata, K., Kimura, T., Kaneko, I., Nakano, T., Yoneyama, K., Takeuchi, Y., Yoshida, S., and Asami, T. 2001. A specific brassinosteroid biosynthesis inhibitor, Brz2001: Evaluation of its effects on Arabidopsis, cress, tobacco, and rice. Planta 213: 716–721.[CrossRef][Medline]

Shenoy, S.K., Drake, M.T., Nelson, C.D., Houtz, D.A., Xiao, K., Madabushi, S., Reiter, E., Premont, R.T., Lichtarge, O., and Lefkowitz, R.J. 2006. -Arrestin-dependent, G protein-independent ERK1/2 activation by the 2 adrenergic receptor. J. Biol. Chem. 281: 1261–1273.[Abstract/Free Full Text]

Shiu, S.H. and Bleecker, A.B. 2001. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc. Natl. Acad. Sci. 98: 10763–10768.[Abstract/Free Full Text]

Vert, G. and Chory, J. 2006. Downstream nuclear events in brassinosteroid signalling. Nature 441: 96–100.[CrossRef][Medline]

Vert, G., Nemhauser, J.L., Geldner, N., Hong, F., and Chory, J. 2005. Molecular mechanisms of steroid hormone signaling in plants. Annu. Rev. Cell Dev. Biol. 21: 177–201.[CrossRef][Medline]

Vieira, A.V., Lamaze, C., and Schmid, S.L. 1996. Control of EGF receptor signaling by clathrin-mediated endocytosis. Science 274: 2086–2089.[Abstract/Free Full Text]

Wang, X. and Chory, J. 2006. Brassinosteroids regulate dissociation of BKI1, a negative regulator of BRI1 signaling, from the plasma membrane. Science 313: 1118–1122.[Abstract/Free Full Text]

Wang, Z.Y., Seto, H., Fujioka, S., Yoshida, S., and Chory, J. 2001. BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature 410: 380–383.[CrossRef][Medline]

Wang, X., Goshe, M.B., Soderblom, E.J., Phinney, B.S., Kuchar, J.A., Li, J., Asami, T., Yoshida, S., Huber, S.C., and Clouse, S.D. 2005. Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase. Plant Cell 17: 1685–1703.[Abstract/Free Full Text]

Wunderlich, W., Fialka, I., Teis, D., Alpi, A., Pfeifer, A., Parton, R.G., Lottspeich, F., and Huber, L.A. 2001. A novel 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold mp1 on a late endosomal/lysosomal compartment. J. Cell Biol. 152: 765–776.[Abstract/Free Full Text]

Yin, Y., Vafeados, D., Tao, Y., Yoshida, S., Asami, T., and Chory, J. 2005. A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. Cell 120: 249–259.[CrossRef][Medline]

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

Signaling from plant endosomes: compartments with something to say!

Natasha Raikhel and Glenn Hicks
Genes & Dev. 2007 21: 1578-1580. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				D. G. Robinson, L. Jiang, and K. Schumacher The Endosomal System of Plants: Charting New and Familiar Territories Plant Physiology, August 1, 2008; 147(4): 1482 - 1492. [Full Text] [PDF]

				D. C. Bassham and M. R. Blatt SNAREs: Cogs and Coordinators in Signaling and Development Plant Physiology, August 1, 2008; 147(4): 1504 - 1515. [Full Text] [PDF]

				J. Aker and S. C. de Vries Plasma Membrane Receptor Complexes Plant Physiology, August 1, 2008; 147(4): 1560 - 1564. [Full Text] [PDF]

				N. Geldner and S. Robatzek Plant Receptors Go Endosomal: A Moving View on Signal Transduction Plant Physiology, August 1, 2008; 147(4): 1565 - 1574. [Full Text] [PDF]

				C. A. Konopka and S. Y. Bednarek Comparison of the Dynamics and Functional Redundancy of the Arabidopsis Dynamin-Related Isoforms DRP1A and DRP1C during Plant Development Plant Physiology, August 1, 2008; 147(4): 1590 - 1602. [Abstract] [Full Text] [PDF]

				S. Robert, S. N. Chary, G. Drakakaki, S. Li, Z. Yang, N. V. Raikhel, and G. R. Hicks Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1 PNAS, June 17, 2008; 105(24): 8464 - 8469. [Abstract] [Full Text] [PDF]

				N. Leborgne-Castel, J. Lherminier, C. Der, J. Fromentin, V. Houot, and F. Simon-Plas The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells Plant Physiology, March 1, 2008; 146(3): 1255 - 1266. [Abstract] [Full Text] [PDF]

				G. S. Ali, K. V. S. K. Prasad, I. Day, and A. S. N. Reddy Ligand-Dependent Reduction in the Membrane Mobility of FLAGELLIN SENSITIVE2, an Arabidopsis Receptor-Like Kinase Plant Cell Physiol., November 1, 2007; 48(11): 1601 - 1611. [Abstract] [Full Text] [PDF]

				N. Raikhel and G. Hicks Signaling from plant endosomes: compartments with something to say! Genes & Dev., July 1, 2007; 21(13): 1578 - 1580. [Full Text] [PDF]

This Article Abstract Full Text (PDF) Supplemental Reearch Data All Versions of this Article: gad.1561307v1 21/13/1598    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geldner, N. Articles by Chory, J. Search for Related Content PubMed PubMed Citation Articles by Geldner, N. Articles by Chory, J. Related Content Related Article Social Bookmarking                   What's this?