![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biochemistry and Biophysics, University of California at San Francisco 94143, USA.
Abstract
The choice of a 3' splice site in Saccharomyces cerevisiae introns involves recognition of a uridine-rich tract upstream of the AG dinucleotide splice junction. By isolating mutants that eliminate the normal preference for uridine-containing 3' splice sites in a cis-competition, we identified a mutation that is an allele of PRP8, prp8-101. This was unexpected because previous analysis has demonstrated that the U5 snRNP protein encoded by PRP8 is required for spliceosome assembly prior to the first catalytic step of splicing. In contrast, the uridine recognition defect caused by the prp8-101 mutation selectively inhibits the second catalytic step of splicing. This defect is seen not only in 3' splice site cis-competitions but also in the splicing of an unusual intron in the TUB3 gene and in the ACT1 intron when utilization of its 3' splice site is rate limiting for splicing. Consistent with a direct role in 3' splice site selection, Prp8 can be cross-linked to the 3' splice site during the splicing reaction. These data demonstrate a novel function for Prp8 in 3' splice site recognition and utilization.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. J. McManus, M. L. Schwartz, S. E. Butcher, and D. A. Brow A dynamic bulge in the U6 RNA internal stem loop functions in spliceosome assembly and activation RNA, December 1, 2007; 13(12): 2252 - 2265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aronova, D. Bacikova, L. B. Crotti, D. S. Horowitz, and B. Schwer Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing RNA, September 1, 2007; 13(9): 1437 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Crotti, D. Bacikova, and D. S. Horowitz The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing Genes & Dev., May 15, 2007; 21(10): 1204 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-L. Boon, T. Auchynnikava, G. Edwalds-Gilbert, J. D. Barrass, A. P. Droop, C. Dez, and J. D. Beggs Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol. Cell. Biol., August 1, 2006; 26(16): 6016 - 6023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Brenner and C. Guthrie Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain RNA, May 1, 2006; 12(5): 862 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. TURNER, C. M. NORMAN, M. J. CHURCHER, and A. J. NEWMAN Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome RNA, March 1, 2006; 12(3): 375 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. BELLARE, A. K. KUTACH, A. K. RINES, C. GUTHRIE, and E. J. SONTHEIMER Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p RNA, February 1, 2006; 12(2): 292 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, J. He, B. Lynn, and B. C. Rymond Interactions of the Yeast SF3b Splicing Factor Mol. Cell. Biol., December 15, 2005; 25(24): 10745 - 10754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Gillis, S. Thomas, S. D. Hansen, and K. B. Kaplan A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis J. Cell Biol., December 5, 2005; 171(5): 773 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Konarska and C. C. Query Insights into the mechanisms of splicing: more lessons from the ribosome Genes & Dev., October 1, 2005; 19(19): 2255 - 2260. [Full Text] [PDF]
|
|
|
|
|
|
T. Villa and C. Guthrie The Isy1p component of the NineTeen Complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing Genes & Dev., August 15, 2005; 19(16): 1894 - 1904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Brenner and C. Guthrie Genetic Analysis Reveals a Role for the C Terminus of the Saccharomyces cerevisiae GTPase Snu114 During Spliceosome Activation Genetics, July 1, 2005; 170(3): 1063 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. GRAINGER and J. D. BEGGS Prp8 protein: At the heart of the spliceosome RNA, May 1, 2005; 11(5): 533 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Du, D. F. Tardiff, M. J. Moore, and M. Rosbash Effects of the U1C L13 mutation and temperature regulation of yeast commitment complex formation PNAS, October 12, 2004; 101(41): 14841 - 14846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang and B. C. Rymond Rds3p Is Required for Stable U2 snRNP Recruitment to the Splicing Apparatus Mol. Cell. Biol., October 15, 2003; 23(20): 7339 - 7349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhang, D. R. Dowd, A. Staal, C. Gu, J. B. Lian, A. J. van Wijnen, G. S. Stein, and P. N. MacDonald Nuclear Coactivator-62 kDa/Ski-interacting Protein Is a Nuclear Matrix-associated Coactivator That May Couple Vitamin D Receptor-mediated Transcription and RNA Splicing J. Biol. Chem., September 12, 2003; 278(37): 35325 - 35336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bartels, H. Urlaub, R. Luhrmann, and P. Fabrizio Mutagenesis Suggests Several Roles of Snu114p in Pre-mRNA Splicing J. Biol. Chem., July 18, 2003; 278(30): 28324 - 28334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. McPheeters and P. Muhlenkamp Spatial Organization of Protein-RNA Interactions in the Branch Site-3' Splice Site Region during pre-mRNA Splicing in Yeast Mol. Cell. Biol., June 15, 2003; 23(12): 4174 - 4186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Chawla, A. K. Sapra, U. Surana, and U. Vijayraghavan Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions Nucleic Acids Res., May 1, 2003; 31(9): 2333 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, K. Hobbs, B. Lynn, and B. C. Rymond The Clf1p Splicing Factor Promotes Spliceosome Assembly through N-terminal Tetratricopeptide Repeat Contacts J. Biol. Chem., February 28, 2003; 278(10): 7875 - 7883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, J. R. Testaverde, C. N. Davis, W. A. Hayajneh, R. Adair, and A. M. Colberg-Poley Human cytomegalovirus UL37 immediate early target minigene RNAs are accurately spliced and polyadenylated J. Gen. Virol., January 1, 2003; 84(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. F. Revers, J. M. Cardone, D. Bonatto, J. Saffi, M. Grey, H. Feldmann, M. Brendel, and J. A. P. Henriques Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1 Nucleic Acids Res., November 15, 2002; 30(22): 4993 - 5003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Libri, F. Duconge, L. Levy, and M. Vinauger A Role for the Psi -U Mismatch in the Recognition of the 5' Splice Site of Yeast Introns by the U1 Small Nuclear Ribonucleoprotein Particle J. Biol. Chem., May 10, 2002; 277(20): 18173 - 18181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Ohi, A. J. Link, L. Ren, J. L. Jennings, W. H. McDonald, and K. L. Gould Proteomics Analysis Reveals Stable Multiprotein Complexes in Both Fission and Budding Yeasts Containing Myb-Related Cdc5p/Cef1p, Novel Pre-mRNA Splicing Factors, and snRNAs Mol. Cell. Biol., April 1, 2002; 22(7): 2011 - 2024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. van Nues and J. D. Beggs Functional Contacts With a Range of Splicing Proteins Suggest a Central Role for Brr2p in the Dynamic Control of the Order of Events in Spliceosomes of Saccharomyces cerevisiae Genetics, April 1, 2001; 157(4): 1451 - 1467. [Abstract] [Full Text]
|
|
|
|
|
|
D. Libri, N. Graziani, C. Saguez, and J. Boulay Multiple roles for the yeast SUB2/yUAP56 gene in splicing Genes & Dev., January 1, 2001; 15(1): 36 - 41. [Abstract] [Full Text]
|
|
|
|
|
|
S. Ben-Yehuda, I. Dix, C. S. Russell, M. McGarvey, J. D. Beggs, and M. Kupiec Genetic and Physical Interactions Between Factors Involved in Both Cell Cycle Progression and Pre-mRNA Splicing in Saccharomyces cerevisiae Genetics, December 1, 2000; 156(4): 1503 - 1517. [Abstract] [Full Text]
|
|
|
|
|
|
A. N. Kuhn and D. A. Brow Suppressors of a Cold-Sensitive Mutation in Yeast U4 RNA Define Five Domains in the Splicing Factor Prp8 That Influence Spliceosome Activation Genetics, August 1, 2000; 155(4): 1667 - 1682. [Abstract] [Full Text]
|
|
|
|
|
|
J. Jiang, D. S. Horowitz, and R.-M. Xu Crystal structure of the functional domain of the splicing factor Prp18 PNAS, March 28, 2000; 97(7): 3022 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. McPheeters, B. Schwer, and P. Muhlenkamp Interaction of the yeast DExH-box RNA helicase Prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing Nucleic Acids Res., March 15, 2000; 28(6): 1313 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, K. Anderson, and M. J. Moore Evidence for a linear search in bimolecular 3' splice site AG selection PNAS, January 18, 2000; 97(2): 593 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ben-Yehuda, C. S. Russell, I. Dix, J. D. Beggs, and M. Kupiec Extensive Genetic Interactions Between PRP8 and PRP17/CDC40, Two Yeast Genes Involved in Pre-mRNA Splicing and Cell Cycle Progression Genetics, January 1, 2000; 154(1): 61 - 71. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Collins and C. Guthrie Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome Genes & Dev., August 1, 1999; 13(15): 1970 - 1982. [Abstract] [Full Text]
|
|
|
|
|
|
M. Siatecka, J. L. Reyes, and M. M. Konarska Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center Genes & Dev., August 1, 1999; 13(15): 1983 - 1993. [Abstract] [Full Text]
|
|
|
|
|
|
V. Segault, C. L. Will, M. Polycarpou-Schwarz, I. W. Mattaj, C. Branlant, and R. Luhrmann Conserved Loop I of U5 Small Nuclear RNA Is Dispensable for Both Catalytic Steps of Pre-mRNA Splicing in HeLa Nuclear Extracts Mol. Cell. Biol., April 1, 1999; 19(4): 2782 - 2790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chua and R. Reed Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing Genes & Dev., April 1, 1999; 13(7): 841 - 850. [Abstract] [Full Text]
|
|
|
|
|
|
S. Lybarger, K. Beickman, V. Brown, N. Dembla-Rajpal, K. Morey, R. Seipelt, and B. C. Rymond Elevated Levels of a U4/U6.U5 snRNP-Associated Protein, Spp381p, Rescue a Mutant Defective in Spliceosome Maturation Mol. Cell. Biol., January 1, 1999; 19(1): 577 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Domon, Z. J. Lorkovic, J. Valcarcel, and W. Filipowicz Multiple Forms of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor U2AF Subunits Expressed in Higher Plants J. Biol. Chem., December 18, 1998; 273(51): 34603 - 34610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Lindsey and M. A. Garcia-Blanco Functional Conservation of the Human Homolog of the Yeast Pre-mRNA Splicing Factor Prp17p J. Biol. Chem., December 4, 1998; 273(49): 32771 - 32775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Achsel, K. Ahrens, H. Brahms, S. Teigelkamp, and R. Lührmann The Human U5-220kD Protein (hPrp8) Forms a Stable RNA-Free Complex with Several U5-Specific Proteins, Including an RNA Unwindase, a Homologue of Ribosomal Elongation Factor EF-2, and a Novel WD-40 Protein Mol. Cell. Biol., November 1, 1998; 18(11): 6756 - 6766. [Abstract] [Full Text]
|
|
|
|
|
|
D. Xu, D. J. Field, S.-J. Tang, A. Moris, B. P. Bobechko, and J. D. Friesen Synthetic Lethality of Yeast slt Mutations with U2 Small Nuclear RNA Mutations Suggests Functional Interactions between U2 and U5 snRNPs That Are Important for Both Steps of Pre-mRNA Splicing Mol. Cell. Biol., April 1, 1998; 18(4): 2055 - 2066. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. L. Raghunathan and C. Guthrie A Spliceosomal Recycling Factor That Reanneals U4 and U6 Small Nuclear Ribonucleoprotein Particles Science, February 6, 1998; 279(5352): 857 - 860. [Abstract] [Full Text]
|
|
|
|
|
|
M. R. McLean and B. C. Rymond Yeast Pre-mRNA Splicing Requires a Pair of U1 snRNP-Associated Tetratricopeptide Repeat Proteins Mol. Cell. Biol., January 1, 1998; 18(1): 353 - 360. [Abstract] [Full Text]
|
|
|
|
|
|
G. Chanfreau, S. A. Elela, M. Ares Jr., and C. Guthrie Alternative 3'-end processing of U5 snRNA by RNase III Genes & Dev., October 15, 1997; 11(20): 2741 - 2751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Anderson and M. J. Moore Bimolecular Exon Ligation by the Human Spliceosome Science, June 13, 1997; 276(5319): 1712 - 1716. [Abstract] [Full Text]
|
|
|
|
|
|
D S Horowitz and A R Krainer A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. Genes & Dev., January 1, 1997; 11(1): 139 - 151. [Abstract] [PDF]
|
|
|
|
|
|
G. Chanfreau, S. M. Noble, and C. Guthrie Essential Yeast Protein with Unexpected Similarity to Subunits of Mammalian Cleavage and Polyadenylation Specificity Factor (CPSF) Science, November 29, 1996; 274(5292): 1511 - 1514. [Abstract] [Full Text]
|
|
|
|
|
|
C. W. Siebel and C. Guthrie The essential yeast RNA binding protein Npl3p is methylated PNAS, November 26, 1996; 93(24): 13641 - 13646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hinz, M. J. Moore, and A. Bindereif Domain Analysis of Human U5 RNA. CAP TRIMETHYLATION, PROTEIN BINDING, AND SPLICEOSOME ASSEMBLY J. Biol. Chem., August 2, 1996; 271(31): 19001 - 19007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H V Colot, F Stutz, and M Rosbash The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex. Genes & Dev., July 1, 1996; 10(13): 1699 - 1708. [Abstract] [PDF]
|
|
|
|
|
|
D J Field and J D Friesen Functionally redundant interactions between U2 and U6 spliceosomal snRNAs. Genes & Dev., February 15, 1996; 10(4): 489 - 501. [Abstract] [PDF]
|
|
|
|
|
|
H. S. Hummel, R. D. Gillespie, and J. Swindle Mutational Analysis of 3' Splice Site Selection during trans-Splicing J. Biol. Chem., November 3, 2000; 275(45): 35522 - 35531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Urlaub, K. Hartmuth, S. Kostka, G. Grelle, and R. Luhrmann A General Approach for Identification of RNA-Protein Cross-linking Sites within Native Human Spliceosomal Small Nuclear Ribonucleoproteins (snRNPs). ANALYSIS OF RNA-PROTEIN CONTACTS IN NATIVE U1 AND U4/U6.U5 snRNPs J. Biol. Chem., December 22, 2000; 275(52): 41458 - 41468. [Abstract] [Full Text] [PDF]
|
|
