QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Norris, J L Articles by Manley, J L Search for Related Content PubMed PubMed Citation Articles by Norris, J L Articles by Manley, J L Social Bookmarking                   What's this?

Research Papers

Regulation of dorsal in cultured cells by Toll and tube: tube function involves a novel mechanism.

Department of Biological Sciences, Columbia University, New York, New York 10027.

Abstract

We described previously a transient cotransfection assay that allows us to study regulation of the Drosophila Dorsal protein (dl) in cultured cells. For example, we showed that over-expression of the Toll transmembrane receptor was sufficient to cause relocalization of dl from the cytoplasm to the nucleus. Here we present data that the tube protein, shown previously by genetic studies to act downstream of Toll, can function in a novel way to enhance dl activity. In the absence of dl, or when dl is cytoplasmic, tube is also found in the cytoplasm of transfected cells. But when dl is localized to the nucleus, so is tube. tube can then function to enhance reporter gene expression, either by cooperation with dl or as a GAL4-tube fusion protein. tube thus appears capable of acting both as a chaperon or escort for dl as it moves to the nucleus, and then as a transcriptional coactivator. We also show that the intracytoplasmic domain of Toll, and specifically the region sharing homology with the interleukin-1 receptor, is sufficient to induce dl-tube nuclear translocation.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X. Hu, Y. Yagi, T. Tanji, S. Zhou, and Y. T. Ip Multimerization and interaction of Toll and Spatzle in Drosophila PNAS, June 22, 2004; 101(25): 9369 - 9374. [Abstract] [Full Text] [PDF]

				B. Shen and J. L. Manley Pelle kinase is activated by autophosphorylation during Toll signaling in Drosophila Development, March 6, 2003; 129(8): 1925 - 1933. [Abstract] [Full Text] [PDF]

				N. Silverman and T. Maniatis NF-{kappa}B signaling pathways in mammalian and insect innate immunity Genes & Dev., September 15, 2001; 15(18): 2321 - 2342. [Full Text] [PDF]

				B. Shen, H. Liu, E. Y. Skolnik, and J. L. Manley Physical and functional interactions between Drosophila TRAF2 and Pelle kinase contribute to Dorsal activation PNAS, July 5, 2001; (2001) 141235698. [Abstract] [Full Text] [PDF]

				K.-i. Inamori, K. Koori, C. Mishima, T. Muta, and S.-i. Kawabata A horseshoe crab receptor structurally related to Drosophila Toll Innate Immunity, October 1, 2000; 6(5): 397 - 399. [Abstract] [PDF]

				V. Bhaskar, S. A. Valentine, and A. J. Courey A Functional Interaction between Dorsal and Components of the Smt3 Conjugation Machinery J. Biol. Chem., February 11, 2000; 275(6): 4033 - 4040. [Abstract] [Full Text] [PDF]

				L. J. Briggs, D. Stein, J. Goltz, V. C. Corrigan, A. Efthymiadis, S. Hubner, and D. A. Jans The cAMP-dependent Protein Kinase Site (Ser312) Enhances Dorsal Nuclear Import through Facilitating Nuclear Localization Sequence/Importin Interaction J. Biol. Chem., August 28, 1998; 273(35): 22745 - 22752. [Abstract] [Full Text] [PDF]

				F. L. Rock, G. Hardiman, J. C. Timans, R. A. Kastelein, and J. F. Bazan A family of human receptors structurally related to Drosophila Toll PNAS, January 20, 1998; 95(2): 588 - 593. [Abstract] [Full Text] [PDF]

				B Shen and J. Manley Phosphorylation modulates direct interactions between the Toll receptor, Pelle kinase and Tube Development, January 12, 1998; 125(23): 4719 - 4728. [Abstract] [PDF]

				J. Yang and R. Steward A multimeric complex and the nuclear targeting of the Drosophila Rel protein Dorsal PNAS, December 23, 1997; 94(26): 14524 - 14529. [Abstract] [Full Text] [PDF]

				D. Edwards, P Towb, and S. Wasserman An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators Development, January 10, 1997; 124(19): 3855 - 3864. [Abstract] [PDF]

				J L Norris and J L Manley Functional interactions between the pelle kinase, Toll receptor, and tube suggest a mechanism for activation of dorsal. Genes & Dev., April 1, 1996; 10(7): 862 - 872. [Abstract] [PDF]

				T. L. Born, D. E. Smith, K. E. Garka, B. R. Renshaw, J. S. Bertles, and J. E. Sims Identification and Characterization of Two Members of a Novel Class of the Interleukin-1 Receptor (IL-1R) Family. DELINEATION OF A NEW CLASS OF IL-1R-RELATED PROTEINS BASED ON SIGNALING J. Biol. Chem., September 22, 2000; 275(39): 29946 - 29954. [Abstract] [Full Text] [PDF]

				B. Shen, H. Liu, E. Y. Skolnik, and J. L. Manley Physical and functional interactions between Drosophila TRAF2 and Pelle kinase contribute to Dorsal activation PNAS, July 17, 2001; 98(15): 8596 - 8601. [Abstract] [Full Text] [PDF]