QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tasheva, E S Articles by Roufa, D J Search for Related Content PubMed PubMed Citation Articles by Tasheva, E S Articles by Roufa, D J Social Bookmarking                   What's this?

Research Papers

Regulation of human RPS14 transcription by intronic antisense RNAs and ribosomal protein S14.

Division of Biology, Kansas State University, Manhattan 66506-4901.

Abstract

RNase protection studies reveal two stable RNAs (250 and 280 nucleotides) transcribed from the antisense strand of the human ribosomal protein gene RPS14's first intron. These transcripts, designated alpha-250 and alpha-280, map to overlapping segments of the intron's 5' sequence. Neither RNA encodes a polypeptide sequence, and both are expressed in all human cells and tissues examined. Although alpha-280 is detected among both the cells' nuclear and cytoplasmic RNAs, the great majority of alpha-250 is found in the cytoplasmic subcellular compartment. As judged by its resistance to high concentrations of alpha-amanitin, cell-free transcription of alpha-250 and alpha-280 appears to involve RNA polymerase I. Tissue culture transfection and cell-free transcription experiments demonstrate that alpha-250 and alpha-280 stimulate S14 mRNA transcription, whereas free ribosomal protein S14 inhibits it. Electrophoretic mobility shift experiments indicate specific binary molecular interactions between r-protein S14, its message and the antisense RNAs. In light of these data, we propose a model for fine regulation of human RPS14 transcription that involves RPS14 intron 1 antisense RNAs as positive effectors and S14 protein as a negative effector.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J.-Q. Ni, L.-P. Liu, D. Hess, J. Rietdorf, and F.-L. Sun Drosophila ribosomal proteins are associated with linker histone H1 and suppress gene transcription Genes & Dev., July 15, 2006; 20(14): 1959 - 1973. [Abstract] [Full Text] [PDF]

				P. A. SCHRODER and M. J. MOORE Association of ribosomal proteins with nascent transcripts in S. cerevisiae RNA, October 1, 2005; 11(10): 1521 - 1529. [Abstract] [Full Text] [PDF]

				M.-D. Yan, C.-C. Hong, G.-M. Lai, A.-L. Cheng, Y.-W. Lin, and S.-E. Chuang Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas Hum. Mol. Genet., June 1, 2005; 14(11): 1465 - 1474. [Abstract] [Full Text] [PDF]

				V. P. Mauro and G. M. Edelman The ribosome filter hypothesis PNAS, September 17, 2002; 99(19): 12031 - 12036. [Abstract] [Full Text] [PDF]

				Y. M. Chau, S. Pando, and H. S. Taylor HOXA11 Silencing and Endogenous HOXA11 Antisense Ribonucleic Acid in the Uterine Endometrium J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2674 - 2680. [Abstract] [Full Text] [PDF]

				T. Shtatland, S. C. Gill, B. E. Javornik, H. E. Johansson, B. S. Singer, O. C. Uhlenbeck, D. A. Zichi, and L. Gold Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX Nucleic Acids Res., November 1, 2000; 28(21): e93 - e93. [Abstract] [Full Text] [PDF]

				M. Nomura Regulation of Ribosome Biosynthesis in Escherichia coli and Saccharomyces cerevisiae: Diversity and Common Principles J. Bacteriol., November 15, 1999; 181(22): 6857 - 6864. [Full Text]

				S. W. Fewell and J. L. Woolford Jr. Ribosomal Protein S14 of Saccharomyces cerevisiae Regulates Its Expression by Binding to RPS14B Pre-mRNA and to 18S rRNA Mol. Cell. Biol., January 1, 1999; 19(1): 826 - 834. [Abstract] [Full Text] [PDF]

				Y. Trifa, I. Privat, J. Gagnon, L. Baeza, and S. Lerbs-Mache The Nuclear RPL4 Gene Encodes a Chloroplast Protein That Co-purifies with the T7-like Transcription Complex as Well as Plastid Ribosomes J. Biol. Chem., February 13, 1998; 273(7): 3980 - 3985. [Abstract] [Full Text] [PDF]

				J. L. Funderburgh, L. M. Corpuz, M. R. Roth, M. L. Funderburgh, E. S. Tasheva, and G. W. Conrad Mimecan, the 25-kDa Corneal Keratan Sulfate Proteoglycan, Is a Product of the Gene Producing Osteoglycin J. Biol. Chem., October 31, 1997; 272(44): 28089 - 28095. [Abstract] [Full Text] [PDF]

				B. P. Zambrowicz, A. Imamoto, S. Fiering, L. A. Herzenberg, W. G. Kerr, and P. Soriano Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta -galactosidase in mouse embryos and hematopoietic cells PNAS, April 15, 1997; 94(8): 3789 - 3794. [Abstract] [Full Text] [PDF]

				V. P. Mauro and G. M. Edelman rRNA-like sequences occur in diverse primary transcripts: Implications for the control of gene expression PNAS, January 21, 1997; 94(2): 422 - 427. [Abstract] [Full Text] [PDF]

				R. Ansaldi, A. Chaboud, and C. Dumas Multiple S Gene Family Members Including Natural Antisense Transcripts Are Differentially Expressed during Development of Maize Flowers J. Biol. Chem., July 28, 2000; 275(31): 24146 - 24155. [Abstract] [Full Text] [PDF]