.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA.
Abstract
Genomic silencing is a fundamental mechanism of transcriptional regulation, yet little is known about conserved mechanisms of silencing. We report here the discovery of four Saccharomyces cerevisiae homologs of the SIR2 silencing gene (HSTs), as well as conservation of this gene family from bacteria to mammals. At least three HST genes can function in silencing; HST1 overexpression restores transcriptional silencing to a sir2 mutant and hst3 hst4 double mutants are defective in telomeric silencing. In addition, HST3 and HST4 together contribute to proper cell cycle progression, radiation resistance, and genomic stability, establishing new connections between silencing and these fundamental cellular processes.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Celic, A. Verreault, and J. D. Boeke Histone H3 K56 Hyperacetylation Perturbs Replisomes and Causes DNA Damage Genetics, August 1, 2008; 179(4): 1769 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Raisner and H. D. Madhani Genomewide Screen for Negative Regulators of Sirtuin Activity in Saccharomyces cerevisiae Reveals 40 Loci and Links to Metabolism Genetics, August 1, 2008; 179(4): 1933 - 1944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Griswold, K. T. Chang, A. P. Runko, M. A. Knight, and K.-T. Min Sir2 mediates apoptosis through JNK-dependent pathways in Drosophila PNAS, June 24, 2008; 105(25): 8673 - 8678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Haldar and R. T. Kamakaka Schizosaccharomyces pombe Hst4 Functions in DNA Damage Response by Regulating Histone H3 K56 Acetylation Eukaryot. Cell, May 1, 2008; 7(5): 800 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Thaminy, B. Newcomb, J. Kim, T. Gatbonton, E. Foss, J. Simon, and A. Bedalov Hst3 Is Regulated by Mec1-dependent Proteolysis and Controls the S Phase Checkpoint and Sister Chromatid Cohesion by Deacetylating Histone H3 at Lysine 56 J. Biol. Chem., December 28, 2007; 282(52): 37805 - 37814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. O. Morton, A. Hayes, M. Wilson, B. M. Rash, S. G. Oliver, and P. Coote Global Phenotype Screening and Transcript Analysis Outlines the Inhibitory Mode(s) of Action of Two Amphibian-Derived, {alpha}-Helical, Cationic Peptides on Saccharomyces cerevisiae Antimicrob. Agents Chemother., November 1, 2007; 51(11): 3948 - 3959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Silvestre, A. Cordeiro-Da-Silva, N. Santarem, B. Vergnes, D. Sereno, and A. Ouaissi SIR2-Deficient Leishmania infantum Induces a Defined IFN-{gamma}/IL-10 Pattern That Correlates with Protection J. Immunol., September 1, 2007; 179(5): 3161 - 3170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sedding and J. Haendeler Do We Age on Sirt1 Expression? Circ. Res., May 25, 2007; 100(10): 1396 - 1398. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mead, R. McCord, L. Youngster, M. Sharma, M. R. Gartenberg, and A. K. Vershon Swapping the Gene-Specific and Regional Silencing Specificities of the Hst1 and Sir2 Histone Deacetylases Mol. Cell. Biol., April 1, 2007; 27(7): 2466 - 2475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Chiani, F. D. Felice, and G. Camilloni SIR2 modifies histone H4-K16 acetylation and affects superhelicity in the ARS region of plasmid chromatin in Saccharomyces cerevisiae Nucleic Acids Res., November 14, 2006; 34(19): 5426 - 5437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fukuda, N. Sano, S. Muto, and M. Horikoshi Simple histone acetylation plays a complex role in the regulation of gene expression. Brief Funct Genomic Proteomic, September 1, 2006; 5(3): 190 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Matecic, K. Martins-Taylor, M. Hickman, J. Tanny, D. Moazed, and S. G. Holmes New Alleles of SIR2 Define Cell-Cycle-Specific Silencing Functions Genetics, August 1, 2006; 173(4): 1939 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Bates, M. Victor, A. K. Jones, Y. Shi, and A. C. Hart Differential contributions of Caenorhabditis elegans histone deacetylases to huntingtin polyglutamine toxicity. J. Neurosci., March 8, 2006; 26(10): 2830 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Lamming, M. Latorre-Esteves, O. Medvedik, S. N. Wong, F. A. Tsang, C. Wang, S.-J. Lin, and D. A. Sinclair HST2 Mediates SIR2-Independent Life-Span Extension by Calorie Restriction Science, September 16, 2005; 309(5742): 1861 - 1864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Lynch, H. B. Fraser, E. Sevastopoulos, J. Rine, and L. N. Rusche Sum1p, the Origin Recognition Complex, and the Spreading of a Promoter-Specific Repressor in Saccharomyces cerevisiae Mol. Cell. Biol., July 15, 2005; 25(14): 5920 - 5932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Starai, J. G. Gardner, and J. C. Escalante-Semerena Residue Leu-641 of Acetyl-CoA Synthetase is Critical for the Acetylation of Residue Lys-609 by the Protein Acetyltransferase Enzyme of Salmonella enterica J. Biol. Chem., July 15, 2005; 280(28): 26200 - 26205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Matsushita, Y. Takami, M. Kimura, S. Tachiiri, M. Ishiai, T. Nakayama, and M. Takata Role of NAD-dependent deacetylases SIRT1 and SIRT2 in radiation and cisplatin-induced cell death in vertebrate cells Genes Cells, April 1, 2005; 10(4): 321 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Gardocki, M. Bakewell, D. Kamath, K. Robinson, K. Borovicka, and J. M. Lopes Genomic Analysis of PIS1 Gene Expression Eukaryot. Cell, March 1, 2005; 4(3): 604 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Freeman-Cook, E. B. Gomez, E. J. Spedale, J. Marlett, S. L. Forsburg, L. Pillus, and P. Laurenson Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+ Genetics, March 1, 2005; 169(3): 1243 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Revollo, A. A. Grimm, and S.-i. Imai The NAD Biosynthesis Pathway Mediated by Nicotinamide Phosphoribosyltransferase Regulates Sir2 Activity in Mammalian Cells J. Biol. Chem., December 3, 2004; 279(49): 50754 - 50763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Buck, C. M. Gallo, and J. S. Smith Diversity in the Sir2 family of protein deacetylases J. Leukoc. Biol., June 1, 2004; 75(6): 939 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Pappas Jr., R. Frisch, and M. Weinreich The NAD+-dependent Sir2p histone deacetylase is a negative regulator of chromosomal DNA replication Genes & Dev., April 1, 2004; 18(7): 769 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Gallo, D. L. Smith Jr., and J. S. Smith Nicotinamide Clearance by Pnc1 Directly Regulates Sir2-Mediated Silencing and Longevity Mol. Cell. Biol., February 1, 2004; 24(3): 1301 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Andrulis, D. C. Zappulla, K. Alexieva-Botcheva, C. Evangelista, and R. Sternglanz One-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors Genetics, January 1, 2004; 166(1): 631 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hirao, J. Posakony, M. Nelson, H. Hruby, M. Jung, J. A. Simon, and A. Bedalov Identification of Selective Inhibitors of NAD+-dependent Deacetylases Using Phenotypic Screens in Yeast J. Biol. Chem., December 26, 2003; 278(52): 52773 - 52782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Bitterman, O. Medvedik, and D. A. Sinclair Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin Microbiol. Mol. Biol. Rev., September 1, 2003; 67(3): 376 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hwang, D. Hocking-Murray, A. K. Bahrami, M. Andersson, J. Rine, and A. Sil Identifying Phase-specific Genes in the Fungal Pathogen Histoplasma capsulatum Using a Genomic Shotgun Microarray Mol. Biol. Cell, June 1, 2003; 14(6): 2314 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Dryden, F. A. Nahhas, J. E. Nowak, A.-S. Goustin, and M. A. Tainsky Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle Mol. Cell. Biol., May 1, 2003; 23(9): 3173 - 3185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Maggio-Hall and J. C. Escalante-Semerena {alpha}-5,6-Dimethylbenzimidazole adenine dinucleotide ({alpha}-DAD), a putative new intermediate of coenzyme B12 biosynthesis in Salmonella typhimurium Microbiology, April 1, 2003; 149(4): 983 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. McCord, M. Pierce, J. Xie, S. Wonkatal, C. Mickel, and A. K. Vershon Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes Mol. Cell. Biol., March 15, 2003; 23(6): 2009 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. U. Astrom, T. W. Cline, and J. Rine The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation Genetics, March 1, 2003; 163(3): 931 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. H. Parsons, S. N. Garcia, L. Pillus, and J. T. Kadonaga Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex PNAS, February 18, 2003; 100(4): 1609 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Starai, H. Takahashi, J. D. Boeke, and J. C. Escalante-Semerena Short-Chain Fatty Acid Activation by Acyl-Coenzyme A Synthetases Requires SIR2 Protein Function in Salmonella enterica and Saccharomyces cerevisiae Genetics, February 1, 2003; 163(2): 545 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. McBurney, X. Yang, K. Jardine, M. Hixon, K. Boekelheide, J. R. Webb, P. M. Lansdorp, and M. Lemieux The Mammalian SIR2{alpha} Protein Has a Role in Embryogenesis and Gametogenesis Mol. Cell. Biol., January 1, 2003; 23(1): 38 - 54. [Abstract] [Full Text]
|
|
|
|
|
|
V. J. Starai, I. Celic, R. N. Cole, J. D. Boeke, and J. C. Escalante-Semerena Sir2-Dependent Activation of Acetyl-CoA Synthetase by Deacetylation of Active Lysine Science, December 20, 2002; 298(5602): 2390 - 2392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pandey, A. Muller, C. A. Napoli, D. A. Selinger, C. S. Pikaard, E. J. Richards, J. Bender, D. W. Mount, and R. A. Jorgensen Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes Nucleic Acids Res., December 1, 2002; 30(23): 5036 - 5055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Newman, J. R. Lundblad, Y. Chen, and S. M. Smolik A Drosophila Homologue of Sir2 Modifies Position-Effect Variegation but Does Not Affect Life Span Genetics, December 1, 2002; 162(4): 1675 - 1685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Rafty, M. T. Schmidt, A.-L. Perraud, A. M. Scharenberg, and J. M. Denu Analysis of O-Acetyl-ADP-ribose as a Target for Nudix ADP-ribose Hydrolases J. Biol. Chem., November 27, 2002; 277(49): 47114 - 47122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Bitterman, R. M. Anderson, H. Y. Cohen, M. Latorre-Esteves, and D. A. Sinclair Inhibition of Silencing and Accelerated Aging by Nicotinamide, a Putative Negative Regulator of Yeast Sir2 and Human SIRT1 J. Biol. Chem., November 15, 2002; 277(47): 45099 - 45107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Onyango, I. Celic, J. M. McCaffery, J. D. Boeke, and A. P. Feinberg SIRT3, a human SIR2 homologue, is an NAD- dependent deacetylase localized to mitochondria PNAS, October 15, 2002; 99(21): 13653 - 13658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cuperus and D. Shore Restoration of Silencing in Saccharomyces cerevisiae by Tethering of a Novel Sir2-Interacting Protein, Esc8 Genetics, October 1, 2002; 162(2): 633 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Garcia and L. Pillus A Unique Class of Conditional sir2 Mutants Displays Distinct Silencing Defects in Saccharomyces cerevisiae Genetics, October 1, 2002; 162(2): 721 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pak and J. Segall Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae Mol. Cell. Biol., September 15, 2002; 22(18): 6417 - 6429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Grunweller and A. E. Ehrenhofer-Murray A Novel Yeast Silencer: The 2{micro} Origin of Saccharomyces cerevisiae Has HST3-, MIG1- and SIR-Dependent Silencing Activity Genetics, September 1, 2002; 162(1): 59 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Kaiser, Z. A. Zimmerman, H. Charbonneau, and P. K. Jackson Disruption of Centrosome Structure, Chromosome Segregation, and Cytokinesis by Misexpression of Human Cdc14A Phosphatase Mol. Biol. Cell, July 1, 2002; 13(7): 2289 - 2300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hoppe, J. C. Tanny, A. D. Rudner, S. A. Gerber, S. Danaie, S. P. Gygi, and D. Moazed Steps in Assembly of Silent Chromatin in Yeast: Sir3-Independent Binding of a Sir2/Sir4 Complex to Silencers and Role for Sir2-Dependent Deacetylation Mol. Cell. Biol., June 15, 2002; 22(12): 4167 - 4180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Jackson and J. M. Denu Structural Identification of 2'- and 3'-O-Acetyl-ADP-ribose as Novel Metabolites Derived from the Sir2 Family of beta -NAD+-dependent Histone/Protein Deacetylases J. Biol. Chem., May 17, 2002; 277(21): 18535 - 18544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Borra, F. J. O'Neill, M. D. Jackson, B. Marshall, E. Verdin, K. R. Foltz, and J. M. Denu Conserved Enzymatic Production and Biological Effect of O-Acetyl-ADP-ribose by Silent Information Regulator 2-like NAD+-dependent Deacetylases J. Biol. Chem., April 5, 2002; 277(15): 12632 - 12641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Huang Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe Nucleic Acids Res., April 1, 2002; 30(7): 1465 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Sandmeier, I. Celic, J. D. Boeke, and J. S. Smith Telomeric and rDNA Silencing in Saccharomyces cerevisiae Are Dependent on a Nuclear NAD+ Salvage Pathway Genetics, March 1, 2002; 160(3): 877 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bedalov, T. Gatbonton, W. P. Irvine, D. E. Gottschling, and J. A. Simon Identification of a small molecule inhibitor of Sir2p PNAS, December 18, 2001; 98(26): 15113 - 15118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.W.M. P. Pijnappel, D. Schaft, A. Roguev, A. Shevchenko, H. Tekotte, M. Wilm, G. Rigaut, B. Seraphin, R. Aasland, and A. F. Stewart The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program Genes & Dev., November 15, 2001; 15(22): 2991 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sutton, R. C. Heller, J. Landry, J. S. Choy, A. Sirko, and R. Sternglanz A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex Mol. Cell. Biol., May 15, 2001; 21(10): 3514 - 3522. [Abstract] [Full Text]
|
|
|
|
|
|
L. N. R. a. J. Rine Conversion of a gene-specific repressor to a regional silencer Genes & Dev., April 15, 2001; 15(8): 955 - 967. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. San-Segundo and G. S. Roeder Role for the Silencing Protein Dot1 in Meiotic Checkpoint Control Mol. Biol. Cell, October 1, 2000; 11(10): 3601 - 3615. [Abstract] [Full Text]
|
|
|
|
|
|
J. S. Smith, C. B. Brachmann, I. Celic, M. A. Kenna, S. Muhammad, V. J. Starai, J. L. Avalos, J. C. Escalante-Semerena, C. Grubmeyer, C. Wolberger, et al. A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family PNAS, June 6, 2000; 97(12): 6658 - 6663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guarente Sir2 links chromatin silencing, metabolism, and aging Genes & Dev., May 1, 2000; 14(9): 1021 - 1026. [Full Text]
|
|
|
|
|
|
M. M. Cockell, S. Perrod, and S. M. Gasser Analysis of Sir2p Domains Required for rDNA and Telomeric Silencing in Saccharomyces cerevisiae Genetics, March 1, 2000; 154(3): 1069 - 1083. [Abstract] [Full Text]
|
|
|
|
|
|
|
