.gif?ad=16248&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Immunology Graduate Program, Tufts University School of Medicine, Boston, Massachusetts 02111.
Abstract
Lymphoid cells transformed by Abelson murine leukemia virus have provided one of the classic models for study of early B-cell development and immunoglobulin rearrangement. Most of these cells have rearranged their heavy-chain locus but not their light chain genes, suggesting that an active v-abl protein interferes with this differentiation step. To test this hypothesis, light-chain gene structure was examined in pre-B cells transformed by temperature-sensitive mutants of the Abelson virus and in derivatives that survive at the nonpermissive temperature because they express a human BCL-2 gene. Our studies reveal that inactivation of the v-abl protein tyrosine kinase triggers high-frequency rearrangement of kappa and lambda light-chain genes. These events are accompanied by marked increases in the expression of RAG-1 and RAG-2 RNAs. These increases occur in the absence of protein synthesis but are dependent on inactivation of the v-abl protein tyrosine kinase. As documented in the accompanying paper (Klug et al., this issue), an active v-abl protein also suppresses the activity of NF-kappa B/rel and expression controlled by the kappa intron enhancer. Together these data demonstrate that the v-abl protein specifically interferes with light-chain gene rearrangement by suppressing at least two pathways essential for this stage of B-cell differentiation and suggest that tyrosine phosphorylation is important in regulating RAG gene expression.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. J. Davis, J. M. Havener, and D. A. Ramsden End-bridging is required for pol {micro} to efficiently promote repair of noncomplementary ends by nonhomologous end joining Nucleic Acids Res., May 1, 2008; 36(9): 3085 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Raval, A. N. Kriatchko, S. Kumar, and P. C. Swanson Evidence for Ku70/Ku80 association with full-length RAG1 Nucleic Acids Res., April 1, 2008; 36(6): 2060 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Davila, F. Liu, L. G. Cowell, A. E. Lieberman, E. Heikamp, A. Patel, and G. Kelsoe Multiple, conserved cryptic recombination signals in VH gene segments: detection of cleavage products only in pro B cells J. Exp. Med., December 24, 2007; 204(13): 3195 - 3208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Fitzsimmons, R. M. Bernstein, E. E. Max, J. A. Skok, and M. A. Shapiro Dynamic Changes in Accessibility, Nuclear Positioning, Recombination, and Transcription at the Ig{kappa} Locus J. Immunol., October 15, 2007; 179(8): 5264 - 5273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ueda, D. Liao, K. Yang, A. Patel, and G. Kelsoe T-Independent Activation-Induced Cytidine Deaminase Expression, Class-Switch Recombination, and Antibody Production by Immature/Transitional 1 B Cells J. Immunol., March 15, 2007; 178(6): 3593 - 3601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. L. K. Lam, C. K. C. Lo, B.-J. Zheng, K.-H. Ko, D. G. Osmond, G. E. Wu, R. Rottapel, and L. Lu Impaired V(D)J recombination and increased apoptosis among B cell precursors in the bone marrow of c-Abl-deficient mice Int. Immunol., March 1, 2007; 19(3): 267 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. V.R. Reddy, E. J. Perkins, and D. A. Ramsden Genomic instability due to V(D)J recombination-associated transposition. Genes & Dev., June 15, 2006; 20(12): 1575 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Jacobsen, O. Ananieva, M. L. Brown, and Y. Chang Growth, Differentiation, and Malignant Transformation of Pre-B Cells Mediated by Inducible Activation of v-Abl Oncogene. J. Immunol., June 1, 2006; 176(11): 6831 - 6838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Klein, N. Feldhahn, J. L. Mooster, M. Sprangers, W.-K. Hofmann, P. Wernet, M. Wartenberg, and M. Muschen Tracing the Pre-B to Immature B Cell Transition in Human Leukemia Cells Reveals a Coordinated Sequence of Primary and Secondary IGK Gene Rearrangement, IGK Deletion, and IGL Gene Rearrangement J. Immunol., January 1, 2005; 174(1): 367 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Perkins, B. L. Kee, and D. A. Ramsden Histone 3 lysine 4 methylation during the pre-B to immature B-cell transition Nucleic Acids Res., March 29, 2004; 32(6): 1942 - 1947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gong, I. Unnikrishnan, A. Raghavan, K. Parmar, and N. Rosenberg Active Akt and Functional p53 Modulate Apoptosis in Abelson Virus-Transformed Pre-B Cells J. Virol., February 15, 2004; 78(4): 1636 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Unnikrishnan and N. Rosenberg Absence of p53 Complements Defects in Abelson Murine Leukemia Virus Signaling J. Virol., June 1, 2003; 77(11): 6208 - 6215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Warren, D. S. Griffin, C. Mainville, and N. Rosenberg The Extreme Carboxyl Terminus of v-Abl Is Required for Lymphoid Cell Transformation by Abelson Virus J. Virol., April 15, 2003; 77(8): 4617 - 4625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Cowell, M. Davila, K. Yang, T. B. Kepler, and G. Kelsoe Prospective Estimation of Recombination Signal Efficiency and Identification of Functional Cryptic Signals in the Genome by Statistical Modeling J. Exp. Med., January 20, 2003; 197(2): 207 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Dillon and M. S. Schlissel Partial restoration of B cell development in Jak-3-/- mice achieved by co-expression of IgH and E{micro}-myc transgenes Int. Immunol., August 1, 2002; 14(8): 893 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Perkins, A. Nair, D. O. Cowley, T. Van Dyke, Y. Chang, and D. A. Ramsden Sensing of intermediates in V(D)J recombination by ATM Genes & Dev., January 15, 2002; 16(2): 159 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Bendall, M. L. Sikes, and E. M. Oltz Transcription Factor NF-{{kappa}}B Regulates Ig{{lambda}} Light Chain Gene Rearrangement J. Immunol., July 1, 2001; 167(1): 264 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Mainville, K. Parmar, I. Unnikrishnan, L. Gong, G. D. Raffel, and N. Rosenberg Temperature-Sensitive Transformation by an Abelson Virus Mutant Encoding an Altered SH2 Domain J. Virol., February 15, 2001; 75(4): 1816 - 1823. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Jenab-Wolcott, D. Rodriguez-Correa, A. H. Reitmair, T. Mak, and N. Rosenberg The Absence of Msh2 Alters Abelson Virus Pre-B-Cell Transformation by Influencing p53 Mutation Mol. Cell. Biol., November 15, 2000; 20(22): 8373 - 8381. [Abstract] [Full Text]
|
|
|
|
|
|
S. Lew, D. Franco, and Y. Chang Activation of V(D)J Recombination Induces the Formation of Interlocus Joints and Hybrid Joints in scid Pre-B-Cell Lines Mol. Cell. Biol., October 1, 2000; 20(19): 7170 - 7177. [Abstract] [Full Text]
|
|
|
|
|
|
S. Aoufouchi, E. Flatter, A. Dahan, A. Faili, B. Bertocci, S. Storck, F. Delbos, L. Cocea, N. Gupta, J.-C. Weill, et al. Two novel human and mouse DNA polymerases of the polX family Nucleic Acids Res., September 15, 2000; 28(18): 3684 - 3693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Fields, B. J. Seufzer, E. M. Oltz, and S. Miyamoto A Switch in Distinct I{kappa}B{alpha} Degradation Mechanisms Mediates Constitutive NF-{kappa}B Activation in Mature B Cells J. Immunol., May 1, 2000; 164(9): 4762 - 4767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Brown and Y. Chang Metabolism of Recombination Coding Ends in scid Cells J. Immunol., April 15, 2000; 164(8): 4135 - 4142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Dillon, M. Mancini, A. Rosen, and M. S. Schlissel Annexin V Binds to Viable B Cells and Colocalizes with a Marker of Lipid Rafts upon B Cell Receptor Activation J. Immunol., February 1, 2000; 164(3): 1322 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cocea, A. De Smet, M. Saghatchian, S. Fillatreau, L. Ferradini, S. Schurmans, J.-C. Weill, and C.-A. Reynaud A Targeted Deletion of a Region Upstream from the Jkappa Cluster Impairs kappa Chain Rearrangement In Cis in Mice and in the 103/bcl2 Cell Line J. Exp. Med., May 3, 1999; 189(9): 1443 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lauring and M. S. Schlissel Distinct Factors Regulate the Murine RAG-2 Promoter in B- and T-Cell Lines Mol. Cell. Biol., April 1, 1999; 19(4): 2601 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chang and M. L. Brown Formation of coding joints in V(D)J recombination-inducible severe combined immune deficient pre-B cell lines PNAS, January 5, 1999; 96(1): 191 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Banerjee and P. Rothman IL-7 Reconstitutes Multiple Aspects of v-Abl-Mediated Signaling J. Immunol., November 1, 1998; 161(9): 4611 - 4617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Radfar, I. Unnikrishnan, H.-W. Lee, R. A. DePinho, and N. Rosenberg p19Arf induces p53-dependent apoptosis during Abelson virus-mediated pre-B cell transformation PNAS, October 27, 1998; 95(22): 13194 - 13199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Whitcomb and P. H. Brodeur Rearrangement and Selection in the Developing V{kappa} Repertoire of the Mouse: An Analysis of the Usage of Two V{kappa} Gene Segments J. Immunol., May 15, 1998; 160(10): 4904 - 4913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Schlissel Structure of Nonhairpin Coding-End DNA Breaks in Cells Undergoing V(D)J Recombination Mol. Cell. Biol., April 1, 1998; 18(4): 2029 - 2037. [Abstract] [Full Text]
|
|
|
|
|
|
J. Y. Reuther, G. W. Reuther, D. Cortez, A. M. Pendergast, and A. S. Baldwin Jr. A requirement for NF-kappa B activation in Bcr-Abl-mediated transformation Genes & Dev., April 1, 1998; 12(7): 968 - 981. [Abstract] [Full Text]
|
|
|
|
|
|
W. Wang, S. Rath, J. M. Durdik, and R. Sen Pentoxifylline Inhibits Ig {kappa} Gene Transcription and Rearrangements in Pre-B Cells J. Immunol., February 15, 1998; 160(4): 1789 - 1795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. Bendall, D. C. Scherer, C. R. Edson, D. W. Ballard, and E. M. Oltz Transcription Factor NF-kappa B Regulates Inducible Oct-2 Gene Expression in Precursor B Lymphocytes J. Biol. Chem., November 14, 1997; 272(46): 28826 - 28828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Han, S. R. Dillon, B. Zheng, M. Shimoda, M. S. Schlissel, and G. Kelsoe V(D)J Recombinase Activity in a Subset of Germinal Center B Lymphocytes Science, October 10, 1997; 278(5336): 301 - 305. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Lin, K. Wong, and K. Calame Repression of c-myc Transcription by Blimp-1, an Inducer of Terminal B Cell Differentiation Science, April 25, 1997; 276(5312): 596 - 599. [Abstract] [Full Text]
|
|
|
|
|
|
Z.-M. Yuan, Y. Huang, T. Ishiko, S. Kharbanda, R. Weichselbaum, and D. Kufe Regulation of DNA damage-induced apoptosis by the c-Abl tyrosine kinase PNAS, February 18, 1997; 94(4): 1437 - 1440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Constantinescu and M. S. Schlissel Changes in Locus-specific V(D)J Recombinase Activity Induced by Immunoglobulin Gene Products during B Cell Development J. Exp. Med., February 17, 1997; 185(4): 609 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dorsch and S. P. Goff Increased sensitivity to apoptotic stimuli in c-abl-deficient progenitor B-cell lines PNAS, November 12, 1996; 93(23): 13131 - 13136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Shi, K Alin, and S P Goff Abl-interactor-1, a novel SH3 protein binding to the carboxy-terminal portion of the Abl protein, suppresses v-abl transforming activity. Genes & Dev., November 1, 1995; 9(21): 2583 - 2597. [Abstract] [PDF]
|
|
|
|
|
|
D A Ramsden and M Gellert Formation and resolution of double-strand break intermediates in V(D)J rearrangement. Genes & Dev., October 1, 1995; 9(19): 2409 - 2420. [Abstract] [PDF]
|
|
|
|
|
|
N. Danial, A Pernis, and P. Rothman Jak-STAT signaling induced by the v-abl oncogene Science, September 29, 1995; 269(5232): 1875 - 1877. [Abstract] [PDF]
|
|
|
|
|
|
C F Eisenbeis, H Singh, and U Storb Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator. Genes & Dev., June 1, 1995; 9(11): 1377 - 1387. [Abstract] [PDF]
|
|
|
|
|
|
L. K. Verkoczy, B.-a. Guinn, and N. L. Berinstein Characterization of the Human B Cell RAG-associated Gene, hBRAG, as a B Cell Receptor Signal-enhancing Glycoprotein Dimer That Associates with Phosphorylated Proteins in Resting B Cells J. Biol. Chem., July 7, 2000; 275(28): 20967 - 20979. [Abstract] [Full Text] [PDF]
|
|
