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Research Papers
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115.
Abstract
The yeast HIS3 TR and TC TATA elements support basal transcription, but only TR can respond to transcriptional activators. Four genes, NOT1(CDC39), NOT2(CDC36), NOT3, NOT4, act as general negative regulators and preferentially affect TC-dependent transcription. Allele-specific suppression, a two-hybrid interaction, and biochemical confractionation suggest that NOT1 and NOT2 are nuclear proteins associated in a discrete, 500-kD complex. NOT4 interacts with NOT1 and NOT3 in the two-hybrid assay, and overexpression of NOT3 or NOT4 suppresses not1 and not2 mutations. Repression by the NOT proteins is not attributable to inhibition of transcriptional activators, does not involve the CYC8/TUP1 negative regulatory complex, and is distinct from repression by nucleosomes or by the SPT4, 5, 6 proteins that affect chromatin structure. We propose that the NOT protein inhibit the basic RNA polymerase II transcription machinery, possibly by affecting TFIID function.
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
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|
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|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 E. L. Gadbois, D. M. Chao, J. C. Reese, M. R. Green, and R. A. Young Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo PNAS, April 1, 1997; 94(7): 3145 - 3150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 L. Stargell and K Struhl The TBP-TFIIA interaction in the response to acidic activators in vivo Science, July 7, 1995; 269(5220): 75 - 78. [Abstract] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
H. Fujimura Yeast homolog of mammalian mitogen-activated protein kinase, FUS3/DAC2 kinase, is required both for cell fusion and for G1 arrest of the cell cycle and morphological changes by the cdc37 mutation J. Cell Sci., January 9, 1994; 107(9): 2617 - 2622. [Abstract] [PDF]
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|
|
|
|
|
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