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Research Papers
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Abstract
Tup1 and Ssn6 transcriptionally repress a wide variety of genes in yeast but do not appear to bind DNA. We provide genetic and biochemical evidence that the DNA-binding protein alpha 2, a regulator of cell-type-specific genes, recruits the Tup1/Ssn6 repressor by directly interacting with Tup1. This interaction is mediated by a region of Tup1 containing seven copies of the WD repeat, a 40 amino acid motif of unknown function found in many other proteins. We have found that a single WD repeat will interact with alpha 2, indicating that the WD repeat is a protein-protein interaction domain. Furthermore, a fragment of Tup1 containing primarily WD repeats provides at least partial repression in the absence of Ssn6, suggesting that the repeats also mediate interaction between Tup1 and other components of the repression machinery.
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|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
 |
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|
|
|
|
|
|
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|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 A Kohler, M. Schmidt-Zachmann, and W. Franke AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats J. Cell Sci., January 5, 1997; 110(9): 1051 - 1062. [Abstract] [PDF]
|
|
|
|
|
|
A Pflugrad, J. Meir, T. Barnes, and D. Miller The Groucho-like transcription factor UNC-37 functions with the neural specificity gene unc-4 to govern motor neuron identity in C. elegans Development, January 5, 1997; 124(9): 1699 - 1709. [Abstract] [PDF]
|
|
|
|
|
|
M. Pinto and C. G. Lobe Products of the grg (Groucho-related Gene) Family Can Dimerize through the Amino-terminal Q Domain J. Biol. Chem., December 20, 1996; 271(51): 33026 - 33031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-M. Yang, C. Inouye, Y. Zeng, D. Bearss, and E. Seto Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3 PNAS, November 12, 1996; 93(23): 12845 - 12850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D G Edmondson, M M Smith, and S Y Roth Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4. Genes & Dev., May 15, 1996; 10(10): 1247 - 1259. [Abstract] [PDF]
|
|
|
|
|
|
D B Starr, W Matsui, J R Thomas, and K R Yamamoto Intracellular receptors use a common mechanism to interpret signaling information at response elements. Genes & Dev., May 15, 1996; 10(10): 1271 - 1283. [Abstract] [PDF]
|
|
|
|
|
|
R L Smith, M J Redd, and A D Johnson The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2. Genes & Dev., December 1, 1995; 9(23): 2903 - 2910. [Abstract] [PDF]
|
|
|
|
|
|
D Tzamarias and K Struhl Distinct TPR motifs of Cyc8 are involved in recruiting the Cyc8-Tup1 corepressor complex to differentially regulated promoters. Genes & Dev., April 1, 1995; 9(7): 821 - 831. [Abstract] [PDF]
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|
|
|
|
|
E Sinn, Z Wang, R Kovelman, and R G Roeder Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors. Genes & Dev., March 15, 1995; 9(6): 675 - 685. [Abstract] [PDF]
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|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
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