.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biology, University of California, San Diego, La Jolla 92093-0347.
Abstract
Purified, reconstituted chromatin templates containing regular, physiological nucleosome spacing were transcribed in vitro by RNA polymerase II along with the Gal4-VP16 activator. When Gal4-VP16 was prebound to DNA before reconstitution of either H1-deficient or H1-containing chromatin, the resulting templates were transcribed with a similar efficiency. Under such conditions, we observed long-range (1000 bp) activation of transcription in vitro with H1-containing chromatin, but not naked DNA templates. When Gal4-VP16 was added to preassembled chromatin, the H1-deficient chromatin was transcriptionally active, whereas the H1-containing chromatin, which possessed properties similar to native chromatin, was transcriptionally inert. We then mimicked DNA replication and chromatin assembly at a replication fork and found that Gal4-VP16 could potentiate transcription during, but not after, replication and assembly of histone H1-containing chromatin. These experiments provide biochemical data that support a DNA replication-dependent mechanism for reconfiguration of chromatin structure and activation of transcription by Gal4-VP16.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. A. Wacker, D. D. Ruhl, E. H. Balagamwala, K. M. Hope, T. Zhang, and W. L. Kraus The DNA Binding and Catalytic Domains of Poly(ADP-Ribose) Polymerase 1 Cooperate in the Regulation of Chromatin Structure and Transcription Mol. Cell. Biol., November 1, 2007; 27(21): 7475 - 7485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Ahn, J. Kim, S. J. Yoo, S. Y. Yoo, H. Roh, J.-H. Choi, M. S. Choi, K. S. Chung, E. J. Han, S. M. Hong, et al. Isolation of 151 Mutants that Have Developmental Defects from T-DNA Tagging Plant Cell Physiol., January 1, 2007; 48(1): 169 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Hertel, G. Langst, W. Horz, and P. Korber Nucleosome Stability at the Yeast PHO5 and PHO8 Promoters Correlates with Differential Cofactor Requirements for Chromatin Opening Mol. Cell. Biol., December 15, 2005; 25(24): 10755 - 10767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W. Lewis, E. L. Beall, T. C. Fleischer, D. Georlette, A. J. Link, and M. R. Botchan Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex Genes & Dev., December 1, 2004; 18(23): 2929 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Sims III, R. Belotserkovskaya, and D. Reinberg Elongation by RNA polymerase II: the short and long of it Genes & Dev., October 15, 2004; 18(20): 2437 - 2468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Korber and W. Horz In Vitro Assembly of the Characteristic Chromatin Organization at the Yeast PHO5 Promoter by a Replication-independent Extract System J. Biol. Chem., August 13, 2004; 279(33): 35113 - 35120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Barbie, B. A. Kudlow, R. Frock, J. Zhao, B. R. Johnson, N. Dyson, E. Harlow, and B. K. Kennedy Nuclear Reorganization of Mammalian DNA Synthesis Prior to Cell Cycle Exit Mol. Cell. Biol., January 15, 2004; 24(2): 595 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Fyodorov, M. D. Blower, G. H. Karpen, and J. T. Kadonaga Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo Genes & Dev., January 15, 2004; 18(2): 170 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Hu, Y. Chen, M. Farooqui, M. C. Thomas, C.-M. Chiang, and L.-N. Wei Suppressive Effect of Receptor-interacting Protein 140 on Coregulator Binding to Retinoic Acid Receptor Complexes, Histone-modifying Enzyme Activity, and Gene Activation J. Biol. Chem., January 2, 2004; 279(1): 319 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mahmoudi, L. M. P. Zuijderduijn, A. Mohd-Sarip, and C. P. Verrijzer GAGA facilitates binding of Pleiohomeotic to a chromatinized Polycomb response element Nucleic Acids Res., July 15, 2003; 31(14): 4147 - 4156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Cheung, A. S. Zarifyan, and W. L. Kraus Histone H1 Represses Estrogen Receptor {alpha} Transcriptional Activity by Selectively Inhibiting Receptor-Mediated Transcription Initiation Mol. Cell. Biol., April 15, 2002; 22(8): 2463 - 2471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Taatjes, A. M. Naar, F. Andel III, E. Nogales, and R. Tjian Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator Science, February 8, 2002; 295(5557): 1058 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Kim, W. S. Lane, and D. Reinberg Human Elongator facilitates RNA polymerase II transcription through chromatin PNAS, January 24, 2002; (2002) 251672198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Martinez, V. B. Palhan, A. Tjernberg, E. S. Lymar, A. M. Gamper, T. K. Kundu, B. T. Chait, and R. G. Roeder Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo Mol. Cell. Biol., October 15, 2001; 21(20): 6782 - 6795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Tyler, K. A. Collins, J. Prasad-Sinha, E. Amiott, M. Bulger, P. J. Harte, R. Kobayashi, and J. T. Kadonaga Interaction between the Drosophila CAF-1 and ASF1 Chromatin Assembly Factors Mol. Cell. Biol., October 1, 2001; 21(19): 6574 - 6584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Manning, T. Ikehara, T. Ito, J. T. Kadonaga, and W. L. Kraus p300 Forms a Stable, Template-Committed Complex with Chromatin: Role for the Bromodomain Mol. Cell. Biol., June 15, 2001; 21(12): 3876 - 3887. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Gelbart, T. Rechsteiner, T. J. Richmond, and T. Tsukiyama Interactions of Isw2 Chromatin Remodeling Complex with Nucleosomal Arrays: Analyses Using Recombinant Yeast Histones and Immobilized Templates Mol. Cell. Biol., March 15, 2001; 21(6): 2098 - 2106. [Abstract] [Full Text]
|
|
|
|
|
|
C. B. Venturi, A. M. Erkine, and D. S. Gross Cell Cycle-Dependent Binding of Yeast Heat Shock Factor to Nucleosomes Mol. Cell. Biol., September 1, 2000; 20(17): 6435 - 6448. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Zhang, N. Vo, and R. H. Goodman Histone Binding Protein RbAp48 Interacts with a Complex of CREB Binding Protein and Phosphorylated CREB Mol. Cell. Biol., July 15, 2000; 20(14): 4970 - 4978. [Abstract] [Full Text]
|
|
|
|
|
|
R. Bagga, S. Michalowski, R. Sabnis, J. D. Griffith, and B. M. Emerson HMG I/Y regulates long-range enhancer-dependent transcription on DNA and chromatin by changes in DNA topology Nucleic Acids Res., July 1, 2000; 28(13): 2541 - 2550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Verreault De novo nucleosome assembly: new pieces in an old puzzle Genes & Dev., June 15, 2000; 14(12): 1430 - 1438. [Full Text]
|
|
|
|
|
|
A. J. Crowe, J. L. Piechan, L. Sang, and M. C. Barton S-Phase Progression Mediates Activation of a Silenced Gene in Synthetic Nuclei Mol. Cell. Biol., June 1, 2000; 20(11): 4169 - 4180. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Kal, T. Mahmoudi, N. B. Zak, and C. P. Verrijzer The Drosophila Brahma complex is an essential coactivator for the trithorax group protein Zeste Genes & Dev., May 1, 2000; 14(9): 1058 - 1071. [Abstract] [Full Text]
|
|
|
|
|
|
W. L. Kraus, E. T. Manning, and J. T. Kadonaga Biochemical Analysis of Distinct Activation Functions in p300 That Enhance Transcription Initiation with Chromatin Templates Mol. Cell. Biol., December 1, 1999; 19(12): 8123 - 8135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Kingston and G. J. Narlikar ATP-dependent remodeling and acetylation as regulators of chromatin fluidity Genes & Dev., September 15, 1999; 13(18): 2339 - 2352. [Full Text]
|
|
|
|
|
|
Z. Liu, J. Wong, S. Y. Tsai, M.-J. Tsai, and B. W. O'Malley Steroid receptor coactivator-1 (SRC-1) enhances ligand-dependent and receptor-dependent cell-free transcription of chromatin PNAS, August 17, 1999; 96(17): 9485 - 9490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Wu, M. C. Thomas, S. Y. Hou, V. Likhite, and C. M. Chiang Isolation of Mouse TFIID and Functional Characterization of TBP and TFIID in Mediating Estrogen Receptor and Chromatin Transcription J. Biol. Chem., August 13, 1999; 274(33): 23480 - 23490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Balasubramanian and R. H. Morse Binding of Gal4p and Bicoid to Nucleosomal Sites in Yeast in the Absence of Replication Mol. Cell. Biol., April 1, 1999; 19(4): 2977 - 2985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Stünkel and H.-U. Bernard The Chromatin Structure of the Long Control Region of Human Papillomavirus Type 16 Represses Viral Oncoprotein Expression J. Virol., March 1, 1999; 73(3): 1918 - 1930. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ikeda, D. J. Steger, A. Eberharter, and J. L. Workman Activation Domain-Specific and General Transcription Stimulation by Native Histone Acetyltransferase Complexes Mol. Cell. Biol., January 1, 1999; 19(1): 855 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pazin, J. W. Hermann, and J. T. Kadonaga Promoter Structure and Transcriptional Activation with Chromatin Templates Assembled In Vitro. A SINGLE Gal4-VP16 DIMER BINDS TO CHROMATIN OR TO DNA WITH COMPARABLE AFFINITY J. Biol. Chem., December 18, 1998; 273(51): 34653 - 34660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. LeRoy, G. Orphanides, W. S. Lane, and D. Reinberg Requirement of RSF and FACT for Transcription of Chromatin Templates in Vitro Science, December 4, 1998; 282(5395): 1900 - 1904. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Näär, P. A. Beaurang, K. M. Robinson, J. D. Oliner, D. Avizonis, S. Scheek, J. Zwicker, J. T. Kadonaga, and R. Tjian Chromatin, TAFs, and a novel multiprotein coactivator are required for synergistic activation by Sp1 and SREBP-1a in vitro Genes & Dev., October 1, 1998; 12(19): 3020 - 3031. [Abstract] [Full Text]
|
|
|
|
|
|
D. S. Geraghty, H. B. Sucic, J. Chen, and D. S. Pederson Evidence That Partial Unwrapping of DNA from Nucleosomes Facilitates the Binding of Heat Shock Factor following DNA Replication in Yeast J. Biol. Chem., August 7, 1998; 273(32): 20463 - 20472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Blackwood and J. T. Kadonaga Going the Distance: A Current View of Enhancer Action Science, July 3, 1998; 281(5373): 60 - 63. [Abstract] [Full Text]
|
|
|
|
|
|
T. Sera and A. P. Wolffe Role of Histone H1 as an Architectural Determinant of Chromatin Structure and as a Specific Repressor of Transcription on Xenopus Oocyte 5S rRNA Genes Mol. Cell. Biol., July 1, 1998; 18(7): 3668 - 3680. [Abstract] [Full Text]
|
|
|
|
|
|
M. Xu, R. T. Simpson, and M. P. Kladde Gal4p-Mediated Chromatin Remodeling Depends on Binding Site Position in Nucleosomes but Does Not Require DNA Replication Mol. Cell. Biol., March 1, 1998; 18(3): 1201 - 1212. [Abstract] [Full Text]
|
|
|
|
|
|
W. A. Krajewski and P. B. Becker Reconstitution of hyperacetylated, DNase I-sensitive chromatin characterized by high conformational flexibility of nucleosomal DNA PNAS, February 17, 1998; 95(4): 1540 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. L. Kraus and J. T. Kadonaga p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation Genes & Dev., February 1, 1998; 12(3): 331 - 342. [Abstract] [Full Text]
|
|
|
|
|
|
P. L. Sheridan, T. P. Mayall, E. Verdin, and K. A. Jones Histone acetyltransferases regulate HIV-1 enhancer activity in vitro Genes & Dev., December 15, 1997; 11(24): 3327 - 3340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wu Chromatin Remodeling and the Control of Gene Expression J. Biol. Chem., November 7, 1997; 272(45): 28171 - 28174. [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pazin, P. Bhargava, E. P. Geiduschek, and J. T. Kadonaga Nucleosome Mobility and the Maintenance of Nucleosome Positioning Science, May 2, 1997; 276(5313): 809 - 812. [Abstract] [Full Text]
|
|
|
|
|
|
T P Mayall, P L Sheridan, M R Montminy, and K A Jones Distinct roles for P-CREB and LEF-1 in TCR alpha enhancer assembly and activation on chromatin templates in vitro. Genes & Dev., April 1, 1997; 11(7): 887 - 899. [Abstract] [PDF]
|
|
|
|
|
|
P D Kaufman, R Kobayashi, and B Stillman Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes & Dev., February 1, 1997; 11(3): 345 - 357. [Abstract] [PDF]
|
|
|
|
|
|
T. Ito, J. K. Tyler, M. Bulger, R. Kobayashi, and J. T. Kadonaga ATP-facilitated Chromatin Assembly with a Nucleoplasmin-like Protein from Drosophila melanogaster J. Biol. Chem., October 4, 1996; 271(40): 25041 - 25048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R E Kingston, C A Bunker, and A N Imbalzano Repression and activation by multiprotein complexes that alter chromatin structure. Genes & Dev., April 15, 1996; 10(8): 905 - 920. [Abstract] [PDF]
|
|
|
|
|
|
M J Pazin, P L Sheridan, K Cannon, Z Cao, J G Keck, J T Kadonaga, and K A Jones NF-kappa B-mediated chromatin reconfiguration and transcriptional activation of the HIV-1 enhancer in vitro. Genes & Dev., January 1, 1996; 10(1): 37 - 49. [Abstract] [PDF]
|
|
|
|
|
|
P L Sheridan, C T Sheline, K Cannon, M L Voz, M J Pazin, J T Kadonaga, and K A Jones Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro. Genes & Dev., September 1, 1995; 9(17): 2090 - 2104. [Abstract] [PDF]
|
|
|
|
|
|
S M Paranjape, A Krumm, and J T Kadonaga HMG17 is a chromatin-specific transcriptional coactivator that increases the efficiency of transcription initiation. Genes & Dev., August 15, 1995; 9(16): 1978 - 1991. [Abstract] [PDF]
|
|
|
|
|
|
M. Pazin, R. Kamakaka, and J. Kadonaga ATP-dependent nucleosome reconfiguration and transcriptional activation from preassembled chromatin templates Science, December 23, 1994; 266(5193): 2007 - 2011. [Abstract] [PDF]
|
|
|
|
|
|
M C Barton and B M Emerson Regulated expression of the beta-globin gene locus in synthetic nuclei. Genes & Dev., October 15, 1994; 8(20): 2453 - 2465. [Abstract] [PDF]
|
|
|
|
|
|
C Klein and K Struhl Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo Science, October 14, 1994; 266(5183): 280 - 282. [Abstract] [PDF]
|
|
|
|
|
|
P Bouvet, S Dimitrov, and A P Wolffe Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1. Genes & Dev., May 15, 1994; 8(10): 1147 - 1159. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Worrad, P. Ram, and R. Schultz Regulation of gene expression in the mouse oocyte and early preimplantation embryo: developmental changes in Sp1 and TATA box-binding protein, TBP Development, January 8, 1994; 120(8): 2347 - 2357. [Abstract] [PDF]
|
|
|
|
|
|
J. Kim, G. Min, and B. Kemper Chromatin Assembly Enhances Binding to the CYP2B1 Phenobarbital-responsive Unit (PBRU) of Nuclear Factor-1, Which Binds Simultaneously with Constitutive Androstane Receptor (CAR)/Retinoid X Receptor (RXR) and Enhances CAR/RXR-mediated Activation of the PBRU J. Biol. Chem., March 2, 2001; 276(10): 7559 - 7567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mizuguchi, A. Vassilev, T. Tsukiyama, Y. Nakatani, and C. Wu ATP-dependent Nucleosome Remodeling and Histone Hyperacetylation Synergistically Facilitate Transcription of Chromatin J. Biol. Chem., April 27, 2001; 276(18): 14773 - 14783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Remboutsika, X. Jacq, and L. Tora Chromatin Is Permissive to TATA-binding Protein (TBP)-mediated Transcription Initiation J. Biol. Chem., April 13, 2001; 276(16): 12781 - 12784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Coustry, Q. Hu, B. de Crombrugghe, and S. N. Maity CBF/NF-Y Functions Both in Nucleosomal Disruption and Transcription Activation of the Chromatin-assembled Topoisomerase IIalpha Promoter. TRANSCRIPTION ACTIVATION BY CBF/NF-Y IN CHROMATIN IS DEPENDENT ON THE PROMOTER STRUCTURE J. Biol. Chem., October 26, 2001; 276(44): 40621 - 40630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Kim, W. S. Lane, and D. Reinberg Human Elongator facilitates RNA polymerase II transcription through chromatin PNAS, February 5, 2002; 99(3): 1241 - 1246. [Abstract] [Full Text] [PDF]
|
|
