![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Using mouse immunoglobulin mu (IgM) pre-mRNA as the model substrate for in vitro splicing, we have explored the role of exon sequences in splicing. We have found that deletion of the 5' portion of exon M2 of the IgM gene abolishes the splicing of its immediately upstream intron. Splicing was restored when a purine-rich sequence found within the deleted region was reinserted into the deletion construct. This M2 exon sequence was able to stimulate the splicing of a heterologous intron of the Drosophila doublesex pre-mRNA that contains a suboptimal 3' splice site sequence. These results show that the IgM M2 exon sequence functions as a splicing enhancer. We found that the assembly of the early splicing complex is stimulated by the M2 exon sequence. In vitro competition experiments show that this stimulatory effect is mediated by the interaction of some trans-acting factors. Our results suggest that the U1 snRNP is one such factor. We propose that recognition of an enhancer exon sequence by the components of splicing machinery plays a vital role in the selection of splice sites, not only for the IgM pre-mRNA but for other pre-mRNAs. We designate such a sequence as exon recognition sequence (ERS).
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Shi, Z. Hu, K. Pabon, and K. W. Scotto Caffeine Regulates Alternative Splicing in a Subset of Cancer-Associated Genes: a Role for SC35 Mol. Cell. Biol., January 15, 2008; 28(2): 883 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Vela, J. M. Hilari, X. Roca, A. M. Munoz-Marmol, A. Ariza, and M. Isamat Multisite and bidirectional exonic splicing enhancer in CD44 alternative exon v3 RNA, December 1, 2007; 13(12): 2312 - 2323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Smith, C. Zhang, J. Wang, S. L. Chew, M. Q. Zhang, and A. R. Krainer An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers Hum. Mol. Genet., August 15, 2006; 15(16): 2490 - 2508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Florea Bioinformatics of alternative splicing and its regulation Brief Bioinform, March 1, 2006; 7(1): 55 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Varon, V. Dutrannoy, G. Weikert, C. Tanzarella, A. Antoccia, L. Stockl, E. Spadoni, L.-A. Kruger, A. d. Masi, K. Sperling, et al. Mild Nijmegen breakage syndrome phenotype due to alternative splicing Hum. Mol. Genet., March 1, 2006; 15(5): 679 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-Q. Xu and W. Mattox Identification of a splicing enhancer in MLH1 using COMPARE, a new assay for determination of relative RNA splicing efficiencies Hum. Mol. Genet., January 15, 2006; 15(2): 329 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Millhouse and J. L. Manley The C-Terminal Domain of RNA Polymerase II Functions as a Phosphorylation-Dependent Splicing Activator in a Heterologous Protein Mol. Cell. Biol., January 15, 2005; 25(2): 533 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Webb, C. M. Romfo, W. J. van Heeckeren, and J. A. Wise Exonic splicing enhancers in fission yeast: functional conservation demonstrates an early evolutionary origin Genes & Dev., January 15, 2005; 19(2): 242 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Arrisi-Mercado, M. Romano, A. F. Muro, and F. E. Baralle An Exonic Splicing Enhancer Offsets the Atypical GU-rich 3' Splice Site of Human Apolipoprotein A-II Exon 3 J. Biol. Chem., September 17, 2004; 279(38): 39331 - 39339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Pacheco, A. Q. Gomes, N. L. Barbosa-Morais, V. Benes, W. Ansorge, M. Wollerton, C. W. Smith, J. Valcarcel, and M. Carmo-Fonseca Diversity of Vertebrate Splicing Factor U2AF35: IDENTIFICATION OF ALTERNATIVELY SPLICED U2AF1 mRNAs J. Biol. Chem., June 25, 2004; 279(26): 27039 - 27049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Caputi, M. Freund, S. Kammler, C. Asang, and H. Schaal A Bidirectional SF2/ASF- and SRp40-Dependent Splicing Enhancer Regulates Human Immunodeficiency Virus Type 1 rev, env, vpu, and nef Gene Expression J. Virol., June 15, 2004; 78(12): 6517 - 6526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. SHEN, J. L.C. KAN, C. GHIGNA, G. BIAMONTI, and M. R. GREEN A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2 RNA, May 1, 2004; 10(5): 787 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Zahler, C. K. Damgaard, J. Kjems, and M. Caputi SC35 and Heterogeneous Nuclear Ribonucleoprotein A/B Proteins Bind to a Juxtaposed Exonic Splicing Enhancer/Exonic Splicing Silencer Element to Regulate HIV-1 tat Exon 2 Splicing J. Biol. Chem., March 12, 2004; 279(11): 10077 - 10084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Scamborova, A. Wong, and J. A. Steitz An Intronic Enhancer Regulates Splicing of the Twintron of Drosophila melanogaster prospero Pre-mRNA by Two Different Spliceosomes Mol. Cell. Biol., March 1, 2004; 24(5): 1855 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. BANERJEE, A. RAHN, B. GAWANDE, S. GUTH, J. VALCARCEL, and R. SINGH The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF65) is essential in vivo but dispensable for activity in vitro RNA, February 1, 2004; 10(2): 240 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. H-F. Zhang, K. A. Heller, I. Hefter, C. S. Leslie, and L. A. Chasin Sequence Information for the Splicing of Human Pre-mRNA Identified by Support Vector Machine Classification Genome Res., December 1, 2003; 13(12): 2637 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. BRUCE, R.W. C. DINGLE, and M. L. PETERSON B-cell and plasma-cell splicing differences: A potential role in regulated immunoglobulin RNA processing RNA, October 1, 2003; 9(10): 1264 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guil, R. Gattoni, M. Carrascal, J. Abian, J. Stevenin, and M. Bach-Elias Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation Mol. Cell. Biol., April 15, 2003; 23(8): 2927 - 2941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kim, H. Shi, D.-k. Lee, and J. T. Lis Specific SR protein-dependent splicing substrates identified through genomic SELEX Nucleic Acids Res., April 1, 2003; 31(7): 1955 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dupont, P. Fontana, C. Bachelot-Loza, J.-L. Reny, I. Bieche, F. Desvard, M. Aiach, and P. Gaussem An intronic polymorphism in the PAR-1 gene is associated with platelet receptor density and the response to SFLLRN Blood, March 1, 2003; 101(5): 1833 - 1840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Hong and T. Li Complex Expression Pattern of RPGR Reveals a Role for Purine-Rich Exonic Splicing Enhancers Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3373 - 3382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yoshimura, Y. Yabuta, T. Ishikawa, and S. Shigeoka Identification of a cis Element for Tissue-specific Alternative Splicing of Chloroplast Ascorbate Peroxidase Pre-mRNA in Higher Plants J. Biol. Chem., October 18, 2002; 277(43): 40623 - 40632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Caputi, R. J. Kendzior Jr., and K. L. Beemon A nonsense mutation in the fibrillin-1 gene of a Marfan syndrome patient induces NMD and disrupts an exonic splicing enhancer Genes & Dev., July 15, 2002; 16(14): 1754 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Young, C. J. DiDonato, D. Hu, R. Kothary, E. J. Androphy, and C. L. Lorson SRp30c-dependent stimulation of survival motor neuron (SMN) exon 7 inclusion is facilitated by a direct interaction with hTra2{beta}1 Hum. Mol. Genet., March 1, 2002; 11(5): 577 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hayakawa, E. Sakashita, E. Ueno, S.-i. Tominaga, T. Hamamoto, Y. Kagawa, and H. Endo Muscle-specific Exonic Splicing Silencer for Exon Exclusion in Human ATP Synthase gamma -Subunit Pre-mRNA J. Biol. Chem., February 22, 2002; 277(9): 6974 - 6984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakajima, Y. Takei, Y. Tanaka, T. Nakagawa, T. Nakata, Y. Noda, M. Setou, and N. Hirokawa Molecular Motor KIF1C Is Not Essential for Mouse Survival and Motor-Dependent Retrograde Golgi Apparatus-to-Endoplasmic Reticulum Transport Mol. Cell. Biol., February 1, 2002; 22(3): 866 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Moseley, P. E. Mullis, M. A. Prince, and J. A. Phillips III An Exon Splice Enhancer Mutation Causes Autosomal Dominant GH Deficiency J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 847 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Deguillien, S.-C. Huang, M. Moriniere, N. Dreumont, E. J. Benz Jr, and F. Baklouti Multiple cis elements regulate an alternative splicing event at 4.1R pre-mRNA during erythroid differentiation Blood, December 15, 2001; 98(13): 3809 - 3816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guth, T. O. Tange, E. Kellenberger, and J. Valcarcel Dual Function for U2AF35 in AG-Dependent Pre-mRNA Splicing Mol. Cell. Biol., November 15, 2001; 21(22): 7673 - 7681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. DiDonato, C. L. Lorson, Y. De Repentigny, L. Simard, C. Chartrand, E. J. Androphy, and R. Kothary Regulation of murine survival motor neuron (Smn) protein levels by modifying Smn exon 7 splicing Hum. Mol. Genet., November 1, 2001; 10(23): 2727 - 2736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Binder, E. Keller, M. Mix, G. G. Massa, W. H. Stokvis-Brantsma, J. M. Wit, and M. B. Ranke Isolated GH Deficiency with Dominant Inheritance: New Mutations, New Insights J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3877 - 3881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Chandler, M. E. McGuffin, and W. Mattox Functionally antagonistic sequences are required for normal autoregulation of Drosophila tra-2 pre-mRNA splicing Nucleic Acids Res., July 15, 2001; 29(14): 3012 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Caudevilla, C. Codony, D. Serra, G. Plasencia, R. Roman, A. Graessmann, G. Asins, M. Bach-Elias, and F. G. Hegardt Localization of an exonic splicing enhancer responsible for mammalian natural trans-splicing Nucleic Acids Res., July 15, 2001; 29(14): 3108 - 3115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ishibashi, T. Mizukami, S. Kanegasaki, L. Motoda, R. Kakinuma, F. Endo, and H. Nunoi Improved superoxide-generating ability by interferon {gamma} due to splicing pattern change of transcripts in neutrophils from patients with a splice site mutation in CYBB gene Blood, July 15, 2001; 98(2): 436 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C.T. van Deutekom, M. Bremmer-Bout, A. A.M. Janson, I. B. Ginjaar, F. Baas, J. T. den Dunnen, and G.-J. B. van Ommen Antisense-induced exon skipping restores dystrophin expression in DMD patient derived muscle cells Hum. Mol. Genet., July 1, 2001; 10(15): 1547 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Bruce and M. L. Peterson Multiple features contribute to efficient constitutive splicing of an unusually large exon Nucleic Acids Res., June 1, 2001; 29(11): 2292 - 2302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lou and R. F. Gagel Alternative Ribonucleic Acid Processing in Endocrine Systems Endocr. Rev., April 1, 2001; 22(2): 205 - 225. [Abstract] [Full Text]
|
|
|
|
|
|
A. Fedorov, S. Saxonov, L. Fedorova, and I. Daizadeh Comparison of intron-containing and intron-lacking human genes elucidates putative exonic splicing enhancers Nucleic Acids Res., April 1, 2001; 29(7): 1464 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Di Blasi, Y. He, L. Morandi, F. Cornelio, P. Guicheney, and M. Mora Mild muscular dystrophy due to a nonsense mutation in the LAMA2 gene resulting in exon skipping Brain, April 1, 2001; 124(4): 698 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. O. F. Penalva, M. J. Lallena, and J. Valcárcel Switch in 3' Splice Site Recognition between Exon Definition and Splicing Catalysis Is Important for Sex-lethal Autoregulation Mol. Cell. Biol., March 15, 2001; 21(6): 1986 - 1996. [Abstract] [Full Text]
|
|
|
|
|
|
M. Romano, R. Marcucci, and F. E. Baralle Splicing of constitutive upstream introns is essential for the recognition of intra-exonic suboptimal splice sites in the thrombopoietin gene Nucleic Acids Res., February 15, 2001; 29(4): 886 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Côté, S. Dupuis, Z.-H. Jiang, and J. Y. Wu Caspase-2 pre-mRNA alternative splicing: Identification of an intronic element containing a decoy 3' acceptor site PNAS, January 23, 2001; (2001) 31564098. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. McCullough and S. M. Berget An Intronic Splicing Enhancer Binds U1 snRNPs To Enhance Splicing and Select 5' Splice Sites Mol. Cell. Biol., December 15, 2000; 20(24): 9225 - 9235. [Abstract] [Full Text]
|
|
|
|
|
|
J. Zhu and A. R. Krainer Pre-mRNA splicing in the absence of an SR protein RS domain Genes & Dev., December 15, 2000; 14(24): 3166 - 3178. [Abstract] [Full Text]
|
|
|
|
|
|
Z.-M. Zheng, E. S. Reid, and C. C. Baker Utilization of the Bovine Papillomavirus Type 1 Late-Stage-Specific Nucleotide 3605 3' Splice Site Is Modulated by a Novel Exonic Bipartite Regulator but Not by an Intronic Purine-Rich Element J. Virol., November 15, 2000; 74(22): 10612 - 10622. [Abstract] [Full Text]
|
|
|
|
|
|
R. P. Carstens, E. J. Wagner, and M. A. Garcia-Blanco An Intronic Splicing Silencer Causes Skipping of the IIIb Exon of Fibroblast Growth Factor Receptor 2 through Involvement of Polypyrimidine Tract Binding Protein Mol. Cell. Biol., October 1, 2000; 20(19): 7388 - 7400. [Abstract] [Full Text]
|
|
|
|
|
|
W. G. Fairbrother and L. A. Chasin Human Genomic Sequences That Inhibit Splicing Mol. Cell. Biol., September 15, 2000; 20(18): 6816 - 6825. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sun and L. A. Chasin Multiple Splicing Defects in an Intronic False Exon Mol. Cell. Biol., September 1, 2000; 20(17): 6414 - 6425. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ichida, Y. Hakamata, M. Hayakawa, E. Ueno, U. Ikeda, K. Shimada, T. Hamamoto, Y. Kagawa, and H. Endo Differential Regulation of Exonic Regulatory Elements for Muscle-specific Alternative Splicing during Myogenesis and Cardiogenesis J. Biol. Chem., May 19, 2000; 275(21): 15992 - 16001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Arima, A. Kowalska, M. Hasegawa, M. Mukoyama, R. Watanabe, M. Kawai, K. Takahashi, T. Iwatsubo, T. Tabira, and N. Sunohara Two brothers with frontotemporal dementia and parkinsonism with an N279K mutation of the tau gene Neurology, May 9, 2000; 54(9): 1787 - 1795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-X. Liu, S. L. Chew, L. Cartegni, M. Q. Zhang, and A. R. Krainer Exonic Splicing Enhancer Motif Recognized by Human SC35 under Splicing Conditions Mol. Cell. Biol., February 1, 2000; 20(3): 1063 - 1071. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Goldstein and W.-T. Chen Identification of an Alternatively Spliced Seprase mRNA That Encodes a Novel Intracellular Isoform J. Biol. Chem., January 28, 2000; 275(4): 2554 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Lorson and E. J. Androphy An exonic enhancer is required for inclusion of an essential exon in the SMA-determining gene SMN Hum. Mol. Genet., January 22, 2000; 9(2): 259 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Kelm Jr., P. K. Elder, and M. J. Getz The Single-stranded DNA-binding Proteins, Puralpha , Purbeta , and MSY1 Specifically Interact with an Exon 3-derived Mouse Vascular Smooth Muscle alpha -Actin Messenger RNA Sequence J. Biol. Chem., December 31, 1999; 274(53): 38268 - 38275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guth, C. Martinez, R. K. Gaur, and J. Valcarcel Evidence for Substrate-Specific Requirement of the Splicing Factor U2AF35 and for Its Function after Polypyrimidine Tract Recognition by U2AF65 Mol. Cell. Biol., December 1, 1999; 19(12): 8263 - 8271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Bourgeois, M. Popielarz, G. Hildwein, and J. Stevenin Identification of a Bidirectional Splicing Enhancer: Differential Involvement of SR Proteins in 5' or 3' Splice Site Activation Mol. Cell. Biol., November 1, 1999; 19(11): 7347 - 7356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Seong, S. Park, and K. Kim Enhanced Splicing of the First Intron from the Gonadotropin-Releasing Hormone (GnRH) Primary Transcript Is a Prerequisite for Mature GnRH Messenger RNA: Presence of GnRH Neuron-Specific Splicing Factors Mol. Endocrinol., November 1, 1999; 13(11): 1882 - 1895. [Abstract] [Full Text]
|
|
|
|
|
|
B. A. Kuo and P. A. Norton Accurate selection of a 5' splice site requires sequences within fibronectin alternative exon B Nucleic Acids Res., October 1, 1999; 27(19): 3945 - 3952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Chew, H.-X. Liu, A. Mayeda, and A. R. Krainer Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing PNAS, September 14, 1999; 96(19): 10655 - 10660. [Abstract] [Full Text] [PDF]
|
|
