![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500.
Abstract
Biochemical and genetic analysis of the embryonic to adult beta-like globin gene switch in chickens has led to the hypothesis that competition between the promoters of the cis-linked epsilon- and beta-globin genes for interaction with a shared enhancer mediates the developmental changes in expression of beta-globin protein isotypes. To test specific predictions of this promoter competition model, a sensitive RNA/polymerase chain reaction assay has been used to investigate the effects of individual beta-globin promoter mutations on expression of the two linked genes in transiently transfected erythroid cells. Mutations that attenuated adult beta-globin transcription resulted concomitantly in a proportional increase in expression of the embryonic epsilon-globin gene. Consistent with the model, mutations disrupting the binding sites for either of two adult stage-specific transcription factors (NF-E4 and beta CTF) indicate that these sites are essential both for induction of beta-globin gene expression and for indirect suppression (through promoter competition) of epsilon-globin transcription in definitive (adult) erythroid cells. These results provide direct evidence that stage-specific transcription factors affect the equilibrium existing between multiple interacting globin cis-regulatory elements. We conclude that promoter competition is an important mechanism through which developmental regulation of chicken beta-globin gene switching is achieved and that such competitive interactions may prove to be generally applicable to the regulation of a variety of other temporally or spatially restricted gene expression patterns.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Shimotsuma, H. Matsuzaki, O. Tanabe, A. D. Campbell, J. D. Engel, A. Fukamizu, and K. Tanimoto Linear Distance from the Locus Control Region Determines {varepsilon}-Globin Transcriptional Activity Mol. Cell. Biol., August 15, 2007; 27(16): 5664 - 5672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Hu, S. Eszterhas, N. Pallazzi, E. E. Bouhassira, J. Fields, O. Tanabe, S. A. Gerber, M. Bulger, J. D. Engel, M. Groudine, et al. Transcriptional interference among the murine {beta}-like globin genes Blood, March 1, 2007; 109(5): 2210 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lin, Q. Chen, L. Lin, S. Smith, and J. Zhou Promoter targeting sequence mediates enhancer interference in the Drosophila embryo PNAS, February 27, 2007; 104(9): 3237 - 3242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Heltemes-Harris, X. Liu, and T. Manser An antibody VH gene that promotes marginal zone B cell development and heavy chain allelic inclusion Int. Immunol., November 1, 2005; 17(11): 1447 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, H. Liu, C. M. Lin, M. I. Aladjem, and E. M. Epner Targeted Deletion of the Chicken {beta}-Globin Regulatory Elements Reveals a Cooperative Gene Silencing Activity J. Biol. Chem., June 17, 2005; 280(24): 23340 - 23348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Swamynathan and J. Piatigorsky Orientation-dependent Influence of an Intergenic Enhancer on the Promoter Activity of the Divergently Transcribed Mouse Shsp/alpha B-crystallin and Mkbp/HspB2 Genes J. Biol. Chem., December 13, 2002; 277(51): 49700 - 49706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Calhoun, A. Stathopoulos, and M. Levine Promoter-proximal tethering elements regulate enhancer-promoter specificity in the DrosophilaAntennapedia complex PNAS, July 9, 2002; 99(14): 9243 - 9247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. N. Cai, Z. Zhang, J. R. Adams, and P. Shen Genomic context modulates insulator activity through promoter competition Development, November 1, 2001; 128(21): 4339 - 4347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bulger and M. Groudine Looping versus linking: toward a model for long-distance gene activation Genes & Dev., October 1, 1999; 13(19): 2465 - 2477. [Full Text]
|
|
|
|
|
|
M. Tanabe, N. Sasai, K. Nagata, X.-D. Liu, P. C. C. Liu, D. J. Thiele, and A. Nakai The Mammalian HSF4 Gene Generates Both an Activator and a Repressor of Heat Shock Genes by Alternative Splicing J. Biol. Chem., September 24, 1999; 274(39): 27845 - 27856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. J. Issa, H. Kantarjian, A. Mohan, S. O'Brien, J. Cortes, S. Pierce, and M. Talpaz Methylation of the ABL1 Promoter in Chronic Myelogenous Leukemia: Lack of Prognostic Significance Blood, March 15, 1999; 93(6): 2075 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Zhou, H Ashe, C Burks, and M Levine Characterization of the transvection mediating region of the abdominal-B locus in Drosophila Development, January 6, 1999; 126(14): 3057 - 3065. [Abstract] [PDF]
|
|
|
|
|
|
J. L. Thorvaldsen, K. L. Duran, and M. S. Bartolomei Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2 Genes & Dev., December 1, 1998; 12(23): 3693 - 3702. [Abstract] [Full Text]
|
|
|
|
|
|
S. Ohtsuki and M. Levine GAGA mediates the enhancer blocking activity of the eve promoter in the Drosophila embryo Genes & Dev., November 1, 1998; 12(21): 3325 - 3330. [Abstract] [Full Text]
|
|
|
|
|
|
B. K. Jones, J. M. Levorse, and S. M. Tilghman Igf2 imprinting does not require its own DNA methylation or H19 RNA Genes & Dev., July 15, 1998; 12(14): 2200 - 2207. [Abstract] [Full Text]
|
|
|
|
|
|
S. Ohtsuki, M. Levine, and H. N. Cai Different core promoters possess distinct regulatory activities in the Drosophila embryo Genes & Dev., February 15, 1998; 12(4): 547 - 556. [Abstract] [Full Text]
|
|
|
|
|
|
S.-K. Lim, J. J. Bieker, C.-S. Lin, and F. Costantini A Shortened Life Span of EKLF-/- Adult Erythrocytes, Due to a Deficiency of beta -Globin Chains, Is Ameliorated by Human gamma -Globin Chains Blood, August 1, 1997; 90(3): 1291 - 1299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Barton, N. Madani, and B. M. Emerson Distal enhancer regulation by promoter derepression in topologically constrained DNA in vitro PNAS, July 8, 1997; 94(14): 7257 - 7262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, J. Bungert, and J. D. Engel Mutation of gene-proximal regulatory elements disrupts human varepsilon -, gamma -, and beta -globin expression in yeast artificial chromosome transgenic mice PNAS, January 7, 1997; 94(1): 169 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Mason, J. A. Grasso, O. Gavrilova, and M. Reitman Identification of Functional Elements of the Chicken epsilon -Globin Promoter Involved in Stage-specific Interaction with the beta /epsilon Enhancer J. Biol. Chem., October 11, 1996; 271(41): 25459 - 25467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Bungert, U Dave, K C Lim, K H Lieuw, J A Shavit, Q Liu, and J D Engel Synergistic regulation of human beta-globin gene switching by locus control region elements HS3 and HS4. Genes & Dev., December 15, 1995; 9(24): 3083 - 3096. [Abstract] [PDF]
|
|
|
|
|
|
P A Leighton, J R Saam, R S Ingram, C L Stewart, and S M Tilghman An enhancer deletion affects both H19 and Igf2 expression. Genes & Dev., September 1, 1995; 9(17): 2079 - 2089. [Abstract] [PDF]
|
|
|
|
|
|
J. Winick, T. Abel, M. W. Leonard, A. M. Michelson, I. Chardon-Loriaux, R. A. Holmgren, T. Maniatis, and J. D. Engel A GATA family transcription factor is expressed along the embryonic dorsoventral axis in Drosophila melanogaster Development, December 1, 1993; 119(4): 1055 - 1065. [Abstract] [PDF]
|
|
|
|
|
|
M C Barton, N Madani, and B M Emerson The erythroid protein cGATA-1 functions with a stage-specific factor to activate transcription of chromatin-assembled beta-globin genes. Genes & Dev., September 1, 1993; 7(9): 1796 - 1809. [Abstract] [PDF]
|
|
|
|
|
|
K Briegel, K C Lim, C Plank, H Beug, J D Engel, and M Zenke Ectopic expression of a conditional GATA-2/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner. Genes & Dev., June 1, 1993; 7(6): 1097 - 1109. [Abstract] [PDF]
|
|
|
|
|
|
M. Leonard, K. Lim, and J. Engel Expression of the chicken GATA factor family during early erythroid development and differentiation Development, January 10, 1993; 119(2): 519 - 531. [Abstract] [PDF]
|
|
