QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Falb, D Articles by Maniatis, T Search for Related Content PubMed PubMed Citation Articles by Falb, D Articles by Maniatis, T Social Bookmarking                   What's this?

Research Papers

A conserved regulatory unit implicated in tissue-specific gene expression in Drosophila and man.

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Abstract

The Drosophila melanogaster alcohol dehydrogenase (Adh) gene is expressed in a specific set of tissues during larval development and in adults. Expression in the adult fat body is controlled by the Adh adult enhancer (AAE). Previous studies identified a negative regulatory element in the AAE and a protein that binds specifically to this sequence [adult enhancer factor-1 (AEF-1)]. Here, we show that the AEF-1-binding site in the AAE and in two other Drosophila fat body enhancers overlaps a sequence recognized by the mammalian transcription factor CCAAT/enhancer-binding protein (C/EBP). Remarkably, these two proteins also bind specifically to overlapping sites in a liver-specific regulatory element of the human Adh gene. Cotransfection experiments in mammalian cells reveal that C/EBP stimulates the activity of the AAE by 50-fold, and this activity can be suppressed by AEF-1. In addition, AEF-1 prevents C/EBP binding in vitro, and displaces prebound C/EBP. Thus, a tissue-specific regulatory unit consisting of one positive and one negative regulatory element has been conserved between Drosophila and man.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Schrem, J. Klempnauer, and J. Borlak Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation Pharmacol. Rev., June 1, 2004; 56(2): 291 - 330. [Abstract] [Full Text] [PDF]

				M. Papaceit, D. Orengo, and E. Juan Sequences Upstream of the Homologous cis-elements of the Adh Adult Enhancer of Drosophila Are Required for Maximal Levels of Adh Gene Transcription in Adults of Scaptodrosophila lebanonensis Genetics, May 1, 2004; 167(1): 289 - 299. [Abstract] [Full Text] [PDF]

				J. E. Oppentocht, W. van Delden, and L. van de Zande Isolation and Characterization of the Genomic Region from Drosophila kuntzei Containing the Adh and Adhr Genes Mol. Biol. Evol., July 1, 2002; 19(7): 1026 - 1040. [Abstract] [Full Text] [PDF]

				V. Brodu, B. Mugat, P. Fichelson, J.-A. Lepesant, and C. Antoniewski A UAS site substitution approach to the in vivo dissection of promoters: interplay between the GATAb activator and the AEF-1 repressor at a Drosophila ecdysone response unit Development, July 1, 2001; 128(13): 2593 - 2602. [Abstract] [Full Text] [PDF]

				M. Z. Kapitskaya, N. T. Dittmer, K. W. Deitsch, W.-L. Cho, D. G. Taylor, T. Leff, and A. S. Raikhel Three Isoforms of a Hepatocyte Nuclear Factor-4 Transcription Factor with Tissue- and Stage-specific Expression in the Adult Mosquito J. Biol. Chem., November 6, 1998; 273(45): 29801 - 29810. [Abstract] [Full Text] [PDF]

				W. Luo and D. G. Skalnik CCAAT Displacement Protein Competes with Multiple Transcriptional Activators for Binding to Four Sites in the Proximal gp91phox Promoter J. Biol. Chem., July 26, 1996; 271(30): 18203 - 18210. [Abstract] [Full Text] [PDF]

				W An and P C Wensink Integrating sex- and tissue-specific regulation within a single Drosophila enhancer. Genes & Dev., January 15, 1995; 9(2): 256 - 266. [Abstract] [PDF]

				S Gonzalez-Crespo and M Levine Related target enhancers for dorsal and NF-kappa B signaling pathways Science, April 8, 1994; 264(5156): 255 - 258. [Abstract] [PDF]

				T Abel, A. Michelson, and T Maniatis A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body Development, January 11, 1993; 119(3): 623 - 633. [Abstract] [PDF]

				K T Coschigano and P C Wensink Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila. Genes & Dev., January 1, 1993; 7(1): 42 - 54. [Abstract] [PDF]

				P Rorth and D J Montell Drosophila C/EBP: a tissue-specific DNA-binding protein required for embryonic development. Genes & Dev., December 1, 1992; 6(12a): 2299 - 2311. [Abstract] [PDF]