![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Howard Hughes Medical Institute, Department of Cardiology, Children's Hospital, Boston, Massachusetts.
Abstract
alpha-Tropomyosin exons 2 and 3 are spliced in a mutually exclusive manner. Exon 3 is included as the default exon in the mRNA of most cell types, whereas exon 2 is only included in the mRNA of smooth muscle cells. The primary determinant for the default selection of exon 3 is the branchpoint/polypyrimidine tract. This element upstream of exon 3 clearly and effectively outcompetes the corresponding element upstream of exon 2. To identify trans-acting factors that bind to this important cis element, we used UV cross-linking to identify a 57-kD protein whose binding characteristics directly correlate with 3'-splice-site selection in cis-competition splicing assays. This protein appears to be identical to polypyrimidine tract-binding protein. In this report we have used oligonucleotides derived from peptide sequences to isolate and sequence cDNA clones encoding this 57.2-kD protein. The primary sequence reveals a novel protein with significant homology to other RNA-binding proteins. Expression of the mRNA is detected in all tissues and cells examined, although its levels exhibit tissue-specific and developmental regulation. Using a biochemical complementation assay, we have found that this protein, along with a 100-kD protein, exists as part of a large complex that is required to rescue splicing from depleted nuclear extracts.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Wang, J. T. Norton, S. Ghosh, J. Kim, K. Fushimi, J. Y. Wu, M. S. Stack, and S. Huang Polypyrimidine Tract-binding Protein (PTB) Differentially Affects Malignancy in a Cell Line-dependent Manner J. Biol. Chem., July 18, 2008; 283(29): 20277 - 20287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-I G. Chen, R. E. Moore, H. Y. Ge, M. K. Young, T. D. Lee, and S. W. Stevens Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors Nucleic Acids Res., June 9, 2007; 35(12): 3928 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Boutz, G. Chawla, P. Stoilov, and D. L. Black MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development Genes & Dev., January 1, 2007; 21(1): 71 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qi, S. Su, M. E. McGuffin, and W. Mattox Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing Nucleic Acids Res., December 4, 2006; 34(21): 6256 - 6263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Crawford and J. G. Patton Activation of {alpha}-Tropomyosin Exon 2 Is Regulated by the SR Protein 9G8 and Heterogeneous Nuclear Ribonucleoproteins H and F Mol. Cell. Biol., December 1, 2006; 26(23): 8791 - 8802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Harris, Z. Zhang, B. Chaubey, and V. N. Pandey Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome Mol. Cell. Proteomics, June 1, 2006; 5(6): 1006 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. SONG, E. TZIMA, K. OCHS, G. BASSILI, H. TRUSHEIM, M. LINDER, K. T. PREISSNER, and M. NIEPMANN Evidence for an RNA chaperone function of polypyrimidine tract-binding protein in picornavirus translation RNA, December 1, 2005; 11(12): 1809 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, Z. Dong, B. Han, Y. Yang, Y. Liu, and J.-T. Zhang Regulation of expression by promoters versus internal ribosome entry site in the 5'-untranslated sequence of the human cyclin-dependent kinase inhibitor p27kip1 Nucleic Acids Res., July 8, 2005; 33(12): 3763 - 3771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cho, J. H. Kim, S. H. Back, and S. K. Jang Polypyrimidine Tract-Binding Protein Enhances the Internal Ribosomal Entry Site-Dependent Translation of p27Kip1 mRNA and Modulates Transition from G1 to S Phase Mol. Cell. Biol., February 15, 2005; 25(4): 1283 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Castelo-Branco, A. Furger, M. Wollerton, C. Smith, A. Moreira, and N. Proudfoot Polypyrimidine Tract Binding Protein Modulates Efficiency of Polyadenylation Mol. Cell. Biol., May 15, 2004; 24(10): 4174 - 4183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Kress, Y. J. Yoon, and K. L. Mowry Nuclear RNP complex assembly initiates cytoplasmic RNA localization J. Cell Biol., April 26, 2004; 165(2): 203 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. BANERJEE, A. RAHN, B. GAWANDE, S. GUTH, J. VALCARCEL, and R. SINGH The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF65) is essential in vivo but dispensable for activity in vitro RNA, February 1, 2004; 10(2): 240 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Thakur, T. Nakamura, G. Calin, A. Russo, J. F. Tamburrino, M. Shimizu, G. Baldassarre, S. Battista, A. Fusco, R. P. Wassell, et al. Regulation of BRCA1 Transcription by Specific Single-Stranded DNA Binding Factors Mol. Cell. Biol., June 1, 2003; 23(11): 3774 - 3787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gooding, P. Kemp, and C. W. J. Smith A Novel Polypyrimidine Tract-binding Protein Paralog Expressed in Smooth Muscle Cells J. Biol. Chem., April 18, 2003; 278(17): 15201 - 15207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Hamilton, A. Genin, R. Q. Cron, and W. F. C. Rigby Delineation of a Novel Pathway That Regulates CD154 (CD40 Ligand) Expression Mol. Cell. Biol., January 15, 2003; 23(2): 510 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Kim, K. Y. Paek, K. Choi, T.-D. Kim, B. Hahm, K.-T. Kim, and S. K. Jang Heterogeneous Nuclear Ribonucleoprotein C Modulates Translation of c-myc mRNA in a Cell Cycle Phase-Dependent Manner Mol. Cell. Biol., January 15, 2003; 23(2): 708 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Kosinski, J. Laughlin, K. Singh, and L. R. Covey A Complex Containing Polypyrimidine Tract-Binding Protein Is Involved in Regulating the Stability of CD40 Ligand (CD154) mRNA J. Immunol., January 15, 2003; 170(2): 979 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bjork, G. Bauren, S. Jin, Y.-G. Tong, T. R. Burglin, U. Hellman, and L. Wieslander A Novel Conserved RNA-binding Domain Protein, RBD-1, Is Essential For Ribosome Biogenesis Mol. Biol. Cell, October 1, 2002; 13(10): 3683 - 3695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Aissouni, C. Perez, B. Calmels, and P. D. Benech The Cleavage/Polyadenylation Activity Triggered by a U-rich Motif Sequence Is Differently Required Depending on the Poly(A) Site Location at Either the First or Last 3'-Terminal Exon of the 2'-5' Oligo(A) Synthetase Gene J. Biol. Chem., September 20, 2002; 277(39): 35808 - 35814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Back, S. Shin, and S. K. Jang Polypyrimidine Tract-binding Proteins Are Cleaved by Caspase-3 during Apoptosis J. Biol. Chem., July 19, 2002; 277(30): 27200 - 27209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Huang, B. V. Zemelman, and I. R. Lehman Endonuclease G, a Candidate Human Enzyme for the Initiation of Genomic Inversion in Herpes Simplex Type 1 Virus J. Biol. Chem., May 31, 2002; 277(23): 21071 - 21079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Back, Y. K. Kim, W. J. Kim, S. Cho, H. R. Oh, J.-E. Kim, and S. K. Jang Translation of Polioviral mRNA Is Inhibited by Cleavage of Polypyrimidine Tract-Binding Proteins Executed by Polioviral 3Cpro J. Virol., March 1, 2002; 76(5): 2529 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hayakawa, E. Sakashita, E. Ueno, S.-i. Tominaga, T. Hamamoto, Y. Kagawa, and H. Endo Muscle-specific Exonic Splicing Silencer for Exon Exclusion in Human ATP Synthase gamma -Subunit Pre-mRNA J. Biol. Chem., February 22, 2002; 277(9): 6974 - 6984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Kamath, D. J. Leary, and S. Huang Nucleocytoplasmic Shuttling of Polypyrimidine Tract-binding Protein Is Uncoupled from RNA Export Mol. Biol. Cell, December 1, 2001; 12(12): 3808 - 3820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Le Guiner, A. Plet, D. Galiana, M.-C. Gesnel, F. Del Gatto-Konczak, and R. Breathnach Polypyrimidine Tract-binding Protein Represses Splicing of a Fibroblast Growth Factor Receptor-2 Gene Alternative Exon through Exon Sequences J. Biol. Chem., November 16, 2001; 276(47): 43677 - 43687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Wagner and M. A. Garcia-Blanco Polypyrimidine Tract Binding Protein Antagonizes Exon Definition Mol. Cell. Biol., May 15, 2001; 21(10): 3281 - 3288. [Full Text]
|
|
|
|
 |
|
 D. M. Standiford, W. T. Sun, M. B. Davis, and C. P. Emerson , Jr. Positive and Negative Intronic Regulatory Elements Control Muscle-Specific Alternative Exon Splicing of Drosophila Myosin Heavy Chain Transcripts Genetics, January 1, 2001; 157(1): 259 - 271. [Abstract] [Full Text]
|
|
|
|
|
|
V. Markovtsov, J. M. Nikolic, J. A. Goldman, C. W. Turck, M.-Y. Chou, and D. L. Black Cooperative Assembly of an hnRNP Complex Induced by a Tissue-Specific Homolog of Polypyrimidine Tract Binding Protein Mol. Cell. Biol., October 15, 2000; 20(20): 7463 - 7479. [Abstract] [Full Text]
|
|
|
|
|
|
R. P. Carstens, E. J. Wagner, and M. A. Garcia-Blanco An Intronic Splicing Silencer Causes Skipping of the IIIb Exon of Fibroblast Growth Factor Receptor 2 through Involvement of Polypyrimidine Tract Binding Protein Mol. Cell. Biol., October 1, 2000; 20(19): 7388 - 7400. [Abstract] [Full Text]
|
|
|
|
|
|
W. G. Fairbrother and L. A. Chasin Human Genomic Sequences That Inhibit Splicing Mol. Cell. Biol., September 15, 2000; 20(18): 6816 - 6825. [Abstract] [Full Text]
|
|
|
|
|
|
H. K. Bayele, R. S. Eisenthal, and P. Towner Complementation of a Glucose Transporter Mutant of Schizosaccharomyces pombe by a Novel Trypanosoma brucei Gene J. Biol. Chem., May 5, 2000; 275(19): 14217 - 14222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Barnard and J. G. Patton Identification and Characterization of a Novel Serine-Arginine-Rich Splicing Regulatory Protein Mol. Cell. Biol., May 1, 2000; 20(9): 3049 - 3057. [Abstract] [Full Text]
|
|
|
|
|
|
R. Gosert, K. H. Chang, R. Rijnbrand, M. Yi, D. V. Sangar, and S. M. Lemon Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo Mol. Cell. Biol., March 1, 2000; 20(5): 1583 - 1595. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. K. Kim and S. K. Jang La protein is required for efficient translation driven by encephalomyocarditis virus internal ribosomal entry site J. Gen. Virol., December 1, 1999; 80(12): 3159 - 3166. [Abstract] [Full Text]
|
|
|
|
|
|
L.-S. Ai and L.-Y. Chau Post-transcriptional Regulation of H-ferritin mRNA. IDENTIFICATION OF A PYRIMIDINE-RICH SEQUENCE IN THE 3'-UNTRANSLATED REGION ASSOCIATED WITH MESSAGE STABILITY IN HUMAN MONOCYTIC THP-1 CELLS J. Biol. Chem., October 15, 1999; 274(42): 30209 - 30214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Chen, F.-M. Boisvert, D. P. Bazett-Jones, and S. Richard A Role for the GSG Domain in Localizing Sam68 to Novel Nuclear Structures in Cancer Cell Lines Mol. Biol. Cell, September 1, 1999; 10(9): 3015 - 3033. [Abstract] [Full Text]
|
|
|
|
|
|
H. Yamamoto, K. Tsukahara, Y. Kanaoka, S. Jinno, and H. Okayama Isolation of a Mammalian Homologue of a Fission Yeast Differentiation Regulator Mol. Cell. Biol., May 1, 1999; 19(5): 3829 - 3841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Shaw-Jackson and T. Michiels Absence of Internal Ribosome Entry Site-Mediated Tissue Specificity in the Translation of a Bicistronic Transgene J. Virol., April 1, 1999; 73(4): 2729 - 2738. [Abstract] [Full Text]
|
|
|
|
|
|
J. Southby, C. Gooding, and C. W. J. Smith Polypyrimidine Tract Binding Protein Functions as a Repressor To Regulate Alternative Splicing of alpha -Actinin Mutally Exclusive Exons Mol. Cell. Biol., April 1, 1999; 19(4): 2699 - 2711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Hunt, J. J. Hsuan, N. Totty, and R. J. Jackson unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA Genes & Dev., February 15, 1999; 13(4): 437 - 448. [Abstract] [Full Text]
|
|
|
|
|
|
H. Lou, D. M. Helfman, R. F. Gagel, and S. M. Berget Polypyrimidine Tract-Binding Protein Positively Regulates Inclusion of an Alternative 3'-Terminal Exon Mol. Cell. Biol., January 1, 1999; 19(1): 78 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Li, P. Huang, S. Park, and M. M. C. Lai Polypyrimidine Tract-Binding Protein Binds to the Leader RNA of Mouse Hepatitis Virus and Serves as a Regulator of Viral Transcription J. Virol., January 1, 1999; 73(1): 772 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. M. Zheng, M. Huynen, and C. C. Baker A pyrimidine-rich exonic splicing suppressor binds multiple RNA splicing factors and inhibits spliceosome assembly PNAS, November 24, 1998; 95(24): 14088 - 14093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Huez, L. Créancier, S. Audigier, M.-C. Gensac, A.-C. Prats, and H. Prats Two Independent Internal Ribosome Entry Sites Are Involved in Translation Initiation of Vascular Endothelial Growth Factor mRNA Mol. Cell. Biol., November 1, 1998; 18(11): 6178 - 6190. [Abstract] [Full Text]
|
|
|
|
|
|
A. Moreira, Y. Takagaki, S. Brackenridge, M. Wollerton, J. L. Manley, and N. J. Proudfoot The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3' end formation by two distinct mechanisms Genes & Dev., August 15, 1998; 12(16): 2522 - 2534. [Abstract] [Full Text]
|
|
|
|
|
|
D. Z. Rudner, K. S. Breger, R. Kanaar, M. D. Adams, and D. C. Rio RNA Binding Activity of Heterodimeric Splicing Factor U2AF: at Least One RS Domain Is Required for High-Affinity Binding Mol. Cell. Biol., July 1, 1998; 18(7): 4004 - 4011. [Abstract] [Full Text]
|
|
|
|
|
|
C. Nanbru, I. Lafon, S. Audigier, M.-C. Gensac, S. Vagner, G. Huez, and A.-C. Prats Alternative Translation of the Proto-oncogene c-myc by an Internal Ribosome Entry Site J. Biol. Chem., December 19, 1997; 272(51): 32061 - 32066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, Z. Dong, and L. R. Bell Sex-lethal Interactions with Protein and RNA. ROLES OF GLYCINE-RICH AND RNA BINDING DOMAINS J. Biol. Chem., August 29, 1997; 272(35): 22227 - 22235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, T. J. Deerinck, M. H. Ellisman, and D. L. Spector The Dynamic Organization of the Perinucleolar Compartment in the Cell Nucleus J. Cell Biol., June 2, 1997; 137(5): 965 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Schultz, C. C. Hardin, and S. M. Lemon Specific Interaction of Glyceraldehyde 3-Phosphate Dehydrogenase with the 5'-Nontranslated RNA of Hepatitis A Virus J. Biol. Chem., June 14, 1996; 271(24): 14134 - 14142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Lou, R F Gagel, and S M Berget An intron enhancer recognized by splicing factors activates polyadenylation. Genes & Dev., January 15, 1996; 10(2): 208 - 219. [Abstract] [PDF]
|
|
|
|
|
|
H Min, R C Chan, and D L Black The generally expressed hnRNP F is involved in a neural-specific pre-mRNA splicing event. Genes & Dev., November 1, 1995; 9(21): 2659 - 2671. [Abstract] [PDF]
|
|
|
|
|
|
X Liu and J E Mertz HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression. Genes & Dev., July 15, 1995; 9(14): 1766 - 1780. [Abstract] [PDF]
|
|
|
|
 |
|
 R Singh, J Valcarcel, and M. Green Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins Science, May 26, 1995; 268(5214): 1173 - 1176. [Abstract] [PDF]
|
|
|
|
 |
|
 I Solovei, H Macgregor, and E Gaginskaya Single stranded nucleic acid binding structures on chicken lampbrush chromosomes J. Cell Sci., January 4, 1995; 108(4): 1391 - 1396. [Abstract] [PDF]
|
|
|
|
|
|
M Tian and T Maniatis A splicing enhancer exhibits both constitutive and regulated activities. Genes & Dev., July 15, 1994; 8(14): 1703 - 1712. [Abstract] [PDF]
|
|
|
|
|
|
C W Siebel, R Kanaar, and D C Rio Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI. Genes & Dev., July 15, 1994; 8(14): 1713 - 1725. [Abstract] [PDF]
|
|
|
|
|
|
D. Bregman, L Du, Y Li, S Ribisi, and S. Warren Cytostellin distributes to nuclear regions enriched with splicing factors J. Cell Sci., January 3, 1994; 107(3): 387 - 396. [Abstract] [PDF]
|
|
|
|
|
|
P. Norton Alternative pre-mRNA splicing: factors involved in splice site selection J. Cell Sci., January 1, 1994; 107(1): 1 - 7. [PDF]
|
|
|
|
|
|
