![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Abstract
We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which the first step of mRNA splicing is inhibited. The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28). The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family of proteins that are thought to function as ATP-dependent RNA helicases. The cold sensitivity is caused by a glycine-to-glutamic acid change in a conserved sequence motif. Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable. In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5. These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. J. McManus, M. L. Schwartz, S. E. Butcher, and D. A. Brow A dynamic bulge in the U6 RNA internal stem loop functions in spliceosome assembly and activation RNA, December 1, 2007; 13(12): 2252 - 2265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-I G. Chen, R. E. Moore, H. Y. Ge, M. K. Young, T. D. Lee, and S. W. Stevens Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors Nucleic Acids Res., June 9, 2007; 35(12): 3928 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Brenner and C. Guthrie Genetic Analysis Reveals a Role for the C Terminus of the Saccharomyces cerevisiae GTPase Snu114 During Spliceosome Activation Genetics, July 1, 2005; 170(3): 1063 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. GRAINGER and J. D. BEGGS Prp8 protein: At the heart of the spliceosome RNA, May 1, 2005; 11(5): 533 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kikuma, M. Ohtsu, T. Utsugi, S. Koga, K. Okuhara, T. Eki, F. Fujimori, and Y. Murakami Dbp9p, a Member of the DEAD Box Protein Family, Exhibits DNA Helicase Activity J. Biol. Chem., May 14, 2004; 279(20): 20692 - 20698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hwang, D. Hocking-Murray, A. K. Bahrami, M. Andersson, J. Rine, and A. Sil Identifying Phase-specific Genes in the Fungal Pathogen Histoplasma capsulatum Using a Genomic Shotgun Microarray Mol. Biol. Cell, June 1, 2003; 14(6): 2314 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Wehner, J. E. G. Gallagher, and S. J. Baserga Components of an Interdependent Unit within the SSU Processome Regulate and Mediate Its Activity Mol. Cell. Biol., October 15, 2002; 22(20): 7258 - 7267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Kuhn, E. M. Reichl, and D. A. Brow Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation PNAS, July 9, 2002; 99(14): 9145 - 9149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Johnson and J. Abelson Characterization of U4 and U6 interactions with the 5' splice site using a S. cerevisiae in vitro trans-splicing system Genes & Dev., August 1, 2001; 15(15): 1957 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Kuhn and D. A. Brow Suppressors of a Cold-Sensitive Mutation in Yeast U4 RNA Define Five Domains in the Splicing Factor Prp8 That Influence Spliceosome Activation Genetics, August 1, 2000; 155(4): 1667 - 1682. [Abstract] [Full Text]
|
|
|
|
|
|
N. S. Amin, K. M. Tuffo, and C. Holm Dominant Mutations in Three Different Subunits of Replication Factor C Suppress Replication Defects in Yeast PCNA Mutants Genetics, December 1, 1999; 153(4): 1617 - 1628. [Abstract] [Full Text]
|
|
|
|
|
|
D.-H. Kim, G. Edwalds-Gilbert, C. Ren, and R.-J. Lin A Mutation in a Methionine tRNA Gene Suppresses the prp2-1 Ts Mutation and Causes a Pre-mRNA Splicing Defect in Saccharomyces cerevisiae Genetics, November 1, 1999; 153(3): 1105 - 1115. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Vidaver, D. M. Fortner, L. S. Loos-Austin, and D. A. Brow Multiple Functions of Saccharomyces cerevisiae Splicing Protein Prp24 in U6 RNA Structural Rearrangements Genetics, November 1, 1999; 153(3): 1205 - 1218. [Abstract] [Full Text]
|
|
|
|
|
|
M. Siatecka, J. L. Reyes, and M. M. Konarska Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center Genes & Dev., August 1, 1999; 13(15): 1983 - 1993. [Abstract] [Full Text]
|
|
|
|
|
|
T. Achsel, K. Ahrens, H. Brahms, S. Teigelkamp, and R. Lührmann The Human U5-220kD Protein (hPrp8) Forms a Stable RNA-Free Complex with Several U5-Specific Proteins, Including an RNA Unwindase, a Homologue of Ribosomal Elongation Factor EF-2, and a Novel WD-40 Protein Mol. Cell. Biol., November 1, 1998; 18(11): 6756 - 6766. [Abstract] [Full Text]
|
|
|
|
|
|
B. Laggerbauer, T. Achsel, and R. Luhrmann The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro PNAS, April 14, 1998; 95(8): 4188 - 4192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Lee and S. J. Baserga Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10 PNAS, December 9, 1997; 94(25): 13536 - 13541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Molnar, A. Crozat, S.-K. Kraeft, Q. P. Dou, L. B. Chen, and A. B. Pardee Association of the mammalian helicase MAH with the pre-mRNA splicing complex PNAS, July 22, 1997; 94(15): 7831 - 7836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Arai, F. Hosoda, H. Kobayashi, K. Arai, Y. Hayashi, N. Kamada, Y. Kaneko, and M. Ohki The inv(11)(p15q22) Chromosome Translocation of De Novo and Therapy-Related Myeloid Malignancies Results in Fusion of the Nucleoporin Gene, NUP98, With the Putative RNA Helicase Gene, DDX10 Blood, June 1, 1997; 89(11): 3936 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. O'Day, G. Dalbadie-McFarland, and J. Abelson The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNA J. Biol. Chem., December 27, 1996; 271(52): 33261 - 33267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Legrain, C Chapon, and F Galisson Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly. Genes & Dev., July 1, 1993; 7(7b): 1390 - 1399. [Abstract] [PDF]
|
|
|
|
|
|
J R Wyatt, E J Sontheimer, and J A Steitz Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes & Dev., December 1, 1992; 6(12b): 2542 - 2553. [Abstract] [PDF]
|
|
|
|
|
|
K W Shannon and C Guthrie Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs. Genes & Dev., May 1, 1991; 5(5): 773 - 785. [Abstract] [PDF]
|
|
|
|
|
|
S. Awasthi, R. Palmer, M. Castro, C. D. Mobarak, and S. W. Ruby New Roles for the Snp1 and Exo84 Proteins in Yeast Pre-mRNA Splicing J. Biol. Chem., August 10, 2001; 276(33): 31004 - 31015. [Abstract] [Full Text] [PDF]
|
|
