![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biological Sciences, Stanford University, California 94305-5020.
Abstract
The genomes of many RNA viruses terminate in a tertiary structure similar to the L-conformation of tRNAs and this structure is recognized by many tRNA-specific enzymes such as aminoacyl-tRNA synthetase. Virtually the entire 3'-untranslated region (UTR) of tobacco mosaic virus (TMV) RNA is involved in an extended tertiary structure containing, in addition to a tRNA-like structure, a pseudoknot domain that lies immediately upstream. Although the functions of these structures are not well understood, they are essential to the virus. We demonstrate that the addition of the 204-base TMV 3'-untranslated region to foreign mRNA constructs can increase gene expression up to 100-fold compared to nonadenylated mRNA. The 3'-UTR of TMV was equal to or greater than a polyadenylated tail in enhancing gene expression in electroporated dicot and monocot protoplasts. The TMV 3'-UTR is functionally similar to a polyadenylated tail in that it increases mRNA stability and translation and must be positioned at the 3' terminus to function efficiently. Similar effects on expression were observed in Chinese hamster ovary cells, demonstrating that the sequence functions in a wide range of eukaryotes. When the extended tertiary structure was dissected, the upstream pseudoknot domain was found to be largely responsible for increasing expression. The inclusion of the tRNA-like structure, however, was important for full regulation.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. S. Kopeina, Z. A. Afonina, K. V. Gromova, V. A. Shirokov, V. D. Vasiliev, and A. S. Spirin Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA Nucleic Acids Res., May 1, 2008; 36(8): 2476 - 2488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li and S.-M. Wong Host-dependent effects of the 3' untranslated region of turnip crinkle virus RNA on accumulation in Hibiscus and Arabidopsis J. Gen. Virol., February 1, 2007; 88(2): 680 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-W. Chiu, R. M. Kinney, and T. W. Dreher Control of Translation by the 5'- and 3'-Terminal Regions of the Dengue Virus Genome J. Virol., July 1, 2005; 79(13): 8303 - 8315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshii, M. Nishikiori, K. Tomita, N. Yoshioka, R. Kozuka, S. Naito, and M. Ishikawa The Arabidopsis Cucumovirus Multiplication 1 and 2 Loci Encode Translation Initiation Factors 4E and 4G J. Virol., June 15, 2004; 78(12): 6102 - 6111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Johanson, T. Hoang, M. Sheth, and L. E. Hyman GRS1, a Yeast tRNA Synthetase with a Role in mRNA 3' End Formation J. Biol. Chem., September 19, 2003; 278(38): 35923 - 35930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Gallie The 5'-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F Nucleic Acids Res., August 1, 2002; 30(15): 3401 - 3411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Zeenko, L. A. Ryabova, A. S. Spirin, H. M. Rothnie, D. Hess, K. S. Browning, and T. Hohn Eukaryotic Elongation Factor 1A Interacts with the Upstream Pseudoknot Domain in the 3' Untranslated Region of Tobacco Mosaic Virus RNA J. Virol., May 3, 2002; 76(11): 5678 - 5691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Neeleman, R. C. L. Olsthoorn, H. J. M. Linthorst, and J. F. Bol Translation of a nonpolyadenylated viral RNA is enhanced by binding of viral coat protein or polyadenylation of the RNA PNAS, November 15, 2001; (2001) 251542798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nozawa, K. Yomogida, N. Kanno, N. Nonomura, T. Miki, A. Okuyama, Y. Nishimune, and M. Nozaki Prostate-specific Transcription Factor hPSE Is Translated Only in Normal Prostate Epithelial Cells Cancer Res., March 1, 2000; 60(5): 1348 - 1352. [Abstract] [Full Text]
|
|
|
|
|
|
G. D. Williams, R.-Y. Chang, and D. A. Brian A Phylogenetically Conserved Hairpin-Type 3' Untranslated Region Pseudoknot Functions in Coronavirus RNA Replication J. Virol., October 1, 1999; 73(10): 8349 - 8355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Magrath and L. E. Hyman A Mutation in GRS1, a Glycyl-tRNA Synthetase, Affects 3'-End Formation in Saccharomyces cerevisiae Genetics, May 1, 1999; 152(1): 129 - 141. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. R. Wells, R. L. Tanguay, H. Le, and D. R. Gallie HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status Genes & Dev., October 15, 1998; 12(20): 3236 - 3251. [Abstract] [Full Text]
|
|
|
|
|
|
S. Gunnery, U. Maivali, and M. B. Mathews Translation of an Uncapped mRNA Involves Scanning J. Biol. Chem., August 22, 1997; 272(34): 21642 - 21646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Tanguay and D. R. Gallie Isolation and Characterization of the 102-Kilodalton RNA-binding Protein That Binds to the 5' and 3' Translational Enhancers of Tobacco Mosaic Virus RNA J. Biol. Chem., June 14, 1996; 271(24): 14316 - 14322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D R Gallie The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency. Genes & Dev., November 1, 1991; 5(11): 2108 - 2116. [Abstract] [PDF]
|
|
|
|
|
|
L. Neeleman, R. C. L. Olsthoorn, H. J. M. Linthorst, and J. F. Bol Translation of a nonpolyadenylated viral RNA is enhanced by binding of viral coat protein or polyadenylation of the RNA PNAS, December 4, 2001; 98(25): 14286 - 14291. [Abstract] [Full Text] [PDF]
|
|
