![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Abstract
We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely unrelated to the consensus TATA element (TATAAA) stimulate transcription with equal or increased efficiency. Transcription mediated by these synthetic elements requires GAL4 and is initiated from normal his3 initiation sites, suggesting that it occurs by a mechanism indistinguishable from that involving wild-type TATA elements. Many, but not all, of these elements act as substrates for yeast TFIID in reconstituted transcription reactions in vitro. These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Bjornsdottir and L. C. Myers Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box Nucleic Acids Res., May 1, 2008; 36(9): 2906 - 2916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Skovorodkin, A. Pimenov, I. Raykhel, B. Schimanski, D. Ammermann, and A. Gunzl {alpha}-Tubulin Minichromosome Promoters in the Stichotrichous Ciliate Stylonychia lemnae Eukaryot. Cell, January 1, 2007; 6(1): 28 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Stewart, J. A. Fischbeck, X. Chen, and L. A. Stargell Non-optimal TATA Elements Exhibit Diverse Mechanistic Consequences J. Biol. Chem., August 11, 2006; 281(32): 22665 - 22673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. Tolic-Norrelykke, M. B. Rasmussen, F. S. Pavone, K. Berg-Sorensen, and L. B. Oddershede Stepwise Bending of DNA by a Single TATA-Box Binding Protein Biophys. J., May 15, 2006; 90(10): 3694 - 3703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Johnston, A. L. Wood, D. J. Bolland, and A. E. Corcoran Complete Sequence Assembly and Characterization of the C57BL/6 Mouse Ig Heavy Chain V Region J. Immunol., April 1, 2006; 176(7): 4221 - 4234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Lee, K. Howcroft, A. Kotekar, H. H. Yang, K. H. Buetow, and D. S. Singer ATG deserts define a novel core promoter subclass Genome Res., September 1, 2005; 15(9): 1189 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. O'Flanagan, G. Paillard, R. Lavery, and A. M. Sengupta Non-additivity in protein-DNA binding Bioinformatics, May 15, 2005; 21(10): 2254 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Grace, M. B. Chandrasekharan, T. C. Hall, and A. J. Crowe Sequence and Spacing of TATA Box Elements Are Critical for Accurate Initiation from the {beta}-Phaseolin Promoter J. Biol. Chem., February 27, 2004; 279(9): 8102 - 8110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Cliften, P. Sudarsanam, A. Desikan, L. Fulton, B. Fulton, J. Majors, R. Waterston, B. A. Cohen, and M. Johnston Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting Science, July 4, 2003; 301(5629): 71 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Stewart, J. van Bergen, and J. Trowsdale Different and Divergent Regulation of the KIR2DL4 and KIR3DL1 Promoters J. Immunol., June 15, 2003; 170(12): 6073 - 6081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Howcroft, A. Raval, J. D. Weissman, A. Gegonne, and D. S. Singer Distinct Transcriptional Pathways Regulate Basal and Activated Major Histocompatibility Complex Class I Expression Mol. Cell. Biol., May 15, 2003; 23(10): 3377 - 3391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chen and D. M. McKearin A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell Development, March 15, 2003; 130(6): 1159 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E.F. Butler and J. T. Kadonaga The RNA polymerase II core promoter: a key component in the regulation of gene expression Genes & Dev., October 15, 2002; 16(20): 2583 - 2592. [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-J. Duan, X. Fang, A. Rohde, H. Han, G. Stamatoyannopoulos, and Q. Li Developmental specificity of recruitment of TBP to the TATA box of the human gamma -globin gene PNAS, April 16, 2002; 99(8): 5509 - 5514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lee, S. Chatterjee, and K. Struhl Genetic Analysis of the Role of Pol II Holoenzyme Components in Repression by the Cyc8-Tup1 Corepressor in Yeast Genetics, August 1, 2000; 155(4): 1535 - 1542. [Abstract] [Full Text]
|
|
|
|
|
|
A. K. Kutach and J. T. Kadonaga The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters Mol. Cell. Biol., July 1, 2000; 20(13): 4754 - 4764. [Abstract] [Full Text]
|
|
|
|
|
|
R. T. Ranallo, K. Struhl, and L. A. Stargell A TATA-Binding Protein Mutant Defective for TFIID Complex Formation In Vivo Mol. Cell. Biol., June 1, 1999; 19(6): 3951 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hoare, J. A. Copland, T. G. Wood, Y.-J. Jeng, M. G. Izban, and M. S. Soloff Identification of a GABP{alpha}/{beta} Binding Site Involved in the Induction of Oxytocin Receptor Gene Expression in Human Breast Cells. Potentiation by c-Fos/c-Jun Endocrinology, May 1, 1999; 140(5): 2268 - 2279. [Abstract] [Full Text]
|
|
|
|
|
|
E. Martinez, H. Ge, Y. Tao, C.-X. Yuan, V. Palhan, and R. G. Roeder Novel Cofactors and TFIIA Mediate Functional Core Promoter Selectivity by the Human TAFII150-Containing TFIID Complex Mol. Cell. Biol., November 1, 1998; 18(11): 6571 - 6583. [Abstract] [Full Text]
|
|
|
|
|
|
N. Li and B. Seetharam A 69-Base Pair Fragment Derived from Human Transcobalamin II Promoter Is Sufficient for High Bidirectional Activity in the Absence of a TATA Box and an Initiator Element in Transfected Cells. ROLE OF AN E BOX IN TRANSCRIPTIONAL ACTIVITY J. Biol. Chem., October 23, 1998; 273(43): 28170 - 28177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hampsey Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 465 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Grishin, M. Rothenberg, M. A. Downs, and K. J. Blumer Mot3, a Zn Finger Transcription Factor That Modulates Gene Expression and Attenuates Mating Pheromone Signaling in Saccharomyces cerevisiae Genetics, June 1, 1998; 149(2): 879 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Meyer, J. Carlstedt-Duke, and D. B. Starr A Weak TATA Box Is a Prerequisite for Glucocorticoid-dependent Repression of the Osteocalcin Gene J. Biol. Chem., December 5, 1997; 272(49): 30709 - 30714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Fickett and A. G. Hatzigeorgiou Eukaryotic Promoter Recognition Genome Res., September 1, 1997; 7(9): 861 - 878. [Full Text] [PDF]
|
|
|
|
|
|
T W Burke and J T Kadonaga Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters. Genes & Dev., March 15, 1996; 10(6): 711 - 724. [Abstract] [PDF]
|
|
|
|
|
|
L. Stargell and K Struhl The TBP-TFIIA interaction in the response to acidic activators in vivo Science, July 7, 1995; 269(5220): 75 - 78. [Abstract] [PDF]
|
|
|
|
|
|
C. Haqq, C. King, E Ukiyama, S Falsafi, T. Haqq, P. Donahoe, and M. Weiss Molecular basis of mammalian sexual determination: activation of Mullerian inhibiting substance gene expression by SRY Science, December 2, 1994; 266(5190): 1494 - 1500. [Abstract] [PDF]
|
|
|
|
|
|
K Struhl Duality of TBP, the universal transcription factor Science, February 25, 1994; 263(5150): 1103 - 1104. [PDF]
|
|
|
|
|
|
D T Auble and S Hahn An ATP-dependent inhibitor of TBP binding to DNA. Genes & Dev., May 1, 1993; 7(5): 844 - 856. [Abstract] [PDF]
|
|
|
|
|
|
J Colgan and J L Manley TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters. Genes & Dev., February 1, 1992; 6(2): 304 - 315. [Abstract] [PDF]
|
|
|
|
|
|
B F Pugh and R Tjian Transcription from a TATA-less promoter requires a multisubunit TFIID complex. Genes & Dev., November 1, 1991; 5(11): 1935 - 1945. [Abstract] [PDF]
|
|
|
|
|
|
I C Taylor, J L Workman, T J Schuetz, and R E Kingston Facilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains. Genes & Dev., July 1, 1991; 5(7): 1285 - 1298. [Abstract] [PDF]
|
|
|
|
|
|
T. Blackwell and H Weintraub Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection Science, November 23, 1990; 250(4984): 1104 - 1110. [Abstract] [PDF]
|
|
|
|
|
|
K. H. Cox, R. Rai, M. Distler, J. R. Daugherty, J. A. Coffman, and T. G. Cooper Saccharomyces cerevisiae GATA Sequences Function as TATA Elements during Nitrogen Catabolite Repression and When Gln3p Is Excluded from the Nucleus by Overproduction of Ure2p J. Biol. Chem., June 2, 2000; 275(23): 17611 - 17618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Boulanger, S. Liu, A. A. Henningsgaard, S. Yu, and T. M. Redmond The Upstream Region of the Rpe65 Gene Confers Retinal Pigment Epithelium-specific Expression in Vivo and in Vitro and Contains Critical Octamer and E-box Binding Sites J. Biol. Chem., September 29, 2000; 275(40): 31274 - 31282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Stewart and L. A. Stargell The Stability of the TFIIA-TBP-DNA Complex Is Dependent on the Sequence of the TATAAA Element J. Biol. Chem., August 3, 2001; 276(32): 30078 - 30084. [Abstract] [Full Text] [PDF]
|
|
