QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Krinke, L Articles by Wulff, D L Search for Related Content PubMed PubMed Citation Articles by Krinke, L Articles by Wulff, D L Social Bookmarking                   What's this?

Research Papers

RNase III-dependent hydrolysis of lambda cII-O gene mRNA mediated by lambda OOP antisense RNA.

Department of Biological Sciences, State University of New York, Albany 12222.

Abstract

The 77-nucleotide OOP antisense RNA of bacteriophage lambda complements lambda cII-O mRNA in a region that includes 55 nucleotides at the 3' end of the cII gene and 22 nucleotides in the intercistronic region between the cII and O genes. OOP RNA, produced from multicopy plasmids, inhibits lambda cII gene expression by approximately 100-fold through an RNase III-dependent mechanism. Using primer extension analysis of cellular RNA isolated from an induced lambda lysogen that contains an OOP DNA plasmid, we have identified a cleavage site in cII-O mRNA within the region of complementarity with OOP RNA, at 13 nucleotides from the 3' end of that region. Ribonuclease protection experiments demonstrate that almost all cII-O mRNA in this overlap region is cleaved when OOP RNA is overproduced in RNase III+ cells but not in RNase III- cells. RNA fragments are detected that extend into the O gene from the cleavage sites, while the sister fragments that extend into the cII gene cannot be detected and must be eliminated by additional hydrolytic events. Differences in levels of uncleaved mRNA between RNase III+ and RNase III- cells are much less at several hundred nucleotides to either side of the target region. An alternate OOP RNA-dependent hydrolytic process occurs in RNase III- cells that results in cleavages in one of two regions, one close to the cleavage site observed in RNase III+ cells, and the second several nucleotides beyond the end of the complementary region between OOP RNA and cII-O mRNA. In this latter case, the fragments that extend into the cII gene are stable, while the sister O gene fragments are destroyed, in direct contrast to the RNase III-dependent process.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Shokeen, S. Patel, T. J. Greenfield, C. Brinkman, and K. E. Weaver Translational Regulation by an Intramolecular Stem-Loop Is Required for Intermolecular RNA Regulation of the par Addiction Module J. Bacteriol., September 15, 2008; 190(18): 6076 - 6083. [Abstract] [Full Text] [PDF]

				B. Lung, A. Zemann, M. J. Madej, M. Schuelke, S. Techritz, S. Ruf, R. Bock, and A. Huttenhofer Identification of small non-coding RNAs from mitochondria and chloroplasts Nucleic Acids Res., September 1, 2006; 34(14): 3842 - 3852. [Abstract] [Full Text] [PDF]

				K. E. Weaver, E. A. Ehli, J. S. Nelson, and S. Patel Antisense RNA Regulation by Stable Complex Formation in the Enterococcus faecalis Plasmid pAD1 par Addiction System J. Bacteriol., October 1, 2004; 186(19): 6400 - 6408. [Abstract] [Full Text] [PDF]

				A. J. Carpousis Degradation of targeted mRNAs in Escherichia coli: regulation by a small antisense RNA Genes & Dev., October 1, 2003; 17(19): 2351 - 2355. [Full Text] [PDF]

				O. Kobiler, S. Koby, D. Teff, D. Court, and A. B. Oppenheim The phage lambda CII transcriptional activator carries a C-terminal domain signaling for rapid proteolysis PNAS, November 12, 2002; 99(23): 14964 - 14969. [Abstract] [Full Text] [PDF]

				H. Wu, A. R. MacLeod, W. F. Lima, and S. T. Crooke Identification and Partial Purification of Human Double Strand RNase Activity. A NOVEL TERMINATING MECHANISM FOR OLIGORIBONUCLEOTIDE ANTISENSE DRUGS J. Biol. Chem., January 30, 1998; 273(5): 2532 - 2542. [Abstract] [Full Text] [PDF]

				H. McAdams and L Shapiro Circuit simulation of genetic networks Science, August 4, 1995; 269(5224): 650 - 656. [Abstract] [PDF]

				K Ranade and A R Poteete A switch in translation mediated by an antisense RNA. Genes & Dev., August 1, 1993; 7(8): 1498 - 1507. [Abstract] [PDF]