Genes and Development

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

GENES & DEVELOPMENT 4:1951-1963, 1990
ISSN 0890-9369
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsieh, P
Right arrow Articles by Camerini-Otero, R D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsieh, P
Right arrow Articles by Camerini-Otero, R D
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Research Papers

Pairing of homologous DNA sequences by proteins: evidence for three-stranded DNA.

P Hsieh, C S Camerini-Otero, and R D Camerini-Otero

Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Abstract

We show that recombinases form joint molecules over very short regions of homology. When these molecules are deproteinized the three strands are in a structure that is surprisingly resistant to dissociation by branch migration, even at elevated temperatures. The joint molecules dissociate at temperatures comparable to those required to melt DNA duplexes of the same length and sequence. We also show that nonenzymatically formed structures of the same length and sequence, which have a free third strand ready to branch migrate, dissociate at much lower temperatures. These results provide compelling evidence that the three DNA strands in the region of pairing are hydrogen bonded to each other. Our observations suggest that such a novel three-stranded DNA molecule, or a structure very similar to it, may be the intermediate in general recombination that is used in the recognition of sequence homology. We discuss some of the structural features implicit in this molecule containing any base sequence and compare them with those manifest in true DNA triple helices containing special sequence motifs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
R. Fulconis, M. Dutreix, and J.-L. Viovy
Numerical Investigation of Sequence Dependence in Homologous Recognition: Evidence for Homology Traps
Biophys. J., June 1, 2005; 88(6): 3770 - 3779.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kusic, S. Kojic, A. Divac, and D. Stefanovic
Noncanonical DNA Elements in the Lamin B2 Origin of DNA Replication
J. Biol. Chem., March 18, 2005; 280(11): 9848 - 9854.
[Abstract] [Full Text] [PDF]

Home page
 DNA ResHome page
Y. Shigemori and M. Oishi
Stable Triple-Stranded DNA Formation and its Application to the SNP Detection
DNA Res, January 1, 2005; 12(6): 441 - 449.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Shigemori and M. Oishi
Specific cleavage of DNA molecules at RecA-mediated triple-strand structure
Nucleic Acids Res., January 2, 2004; 32(1): e4 - e4.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. G. Rossler, A. Straka, and H. Stahl
Rearrangement of structured RNA via branch migration structures catalysed by the highly related DEAD-box proteins p68 and p72
Nucleic Acids Res., May 15, 2001; 29(10): 2088 - 2096.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Nishinaka, A. Shinohara, Y. Ito, S. Yokoyama, and T. Shibata
Base pair switching by interconversion of sugar puckers in DNA extended by proteins of RecA-family: A model for homology search in homologous genetic recombination
PNAS, September 15, 1998; 95(19): 11071 - 11076.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Norden, P. Wittung-Stafshede, C. Ellouze, H.-K. Kim, K. Mortensen, and M. Takahashi
Base Orientation of Second DNA in RecA·DNA Filaments. ANALYSIS BY COMBINATION OF LINEAR DICHROISM AND SMALL ANGLE NEUTRON SCATTERING IN FLOW-ORIENTED SOLUTION
J. Biol. Chem., June 19, 1998; 273(25): 15682 - 15686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. C. Wong, S.-K. Chiu, and S. A. Chow
The Role of Negative Superhelicity and Length of Homology in the Formation of Paranemic Joints Promoted by RecA Protein
J. Biol. Chem., May 15, 1998; 273(20): 12120 - 12127.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Nishinaka, Y. Ito, S. Yokoyama, and T. Shibata
An extended DNA structure through deoxyribose-base stacking induced by RecA protein
PNAS, June 24, 1997; 94(13): 6623 - 6628.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R B Tracy and S C Kowalczykowski
In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein.
Genes & Dev., August 1, 1996; 10(15): 1890 - 1903.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
K Adzuma
Stable synapsis of homologous DNA molecules mediated by the Escherichia coli RecA protein involves local exchange of DNA strands.
Genes & Dev., September 1, 1992; 6(9): 1679 - 1694.
[Abstract] [PDF]

Home page
 ScienceHome page
L. Ferrin and R. Camerini-Otero
Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage
Science, December 6, 1991; 254(5037): 1494 - 1497.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genome Res. Learn. Mem.
Protein Science RNA Genes Dev.