![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115.
Abstract
A model pre-mRNA substrate was used to carry out a detailed investigation of the functional organization of sequences at the 3' end of mammalian introns. This analysis revealed a difference in the sequence requirements for the first and second steps of the splicing reaction (lariat formation and exon ligation, respectively). Maximal efficiencies of lariat formation require a pyrimidine stretch directly adjacent to the branch site. In addition, efficient lariat formation can be specified in at least two distinct ways, one that requires the AG dinucleotide at the 3' splice junction, and the other that does not: If the pyrimidine stretch is short (14 nucleotides), an adjacent AG is essential; in contrast, the AG is not required in the presence of a long pyrimidine stretch (26 nucleotides). In a pre-mRNA containing a long pyrimidine stretch, efficient lariat formation is observed when the branch site is located greater than 100 nucleotides upstream from the AG or when the AG is preceded by a purine stretch. Although splicing usually takes place at the first AG downstream from the branch site, both distance and sequence play roles in the efficiency of this reaction.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Izquierdo Fas Splicing Regulation during Early Apoptosis Is Linked to Caspase-mediated Cleavage of U2AF65 Mol. Biol. Cell, August 1, 2008; 19(8): 3299 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Pacheco, M. B. Coelho, J. M. P. Desterro, I. Mollet, and M. Carmo-Fonseca In Vivo Requirement of the Small Subunit of U2AF for Recognition of a Weak 3' Splice Site Mol. Cell. Biol., November 1, 2006; 26(21): 8183 - 8190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Pacheco, L. F. Moita, A. Q. Gomes, N. Hacohen, and M. Carmo-Fonseca RNA Interference Knockdown of hU2AF35 Impairs Cell Cycle Progression and Modulates Alternative Splicing of Cdc25 Transcripts Mol. Biol. Cell, October 1, 2006; 17(10): 4187 - 4199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Vorechovsky Aberrant 3' splice sites in human disease genes: mutation pattern, nucleotide structure and comparison of computational tools that predict their utilization Nucleic Acids Res., September 15, 2006; (2006) gkl535v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-B. Wang and V. Brendel Molecular Characterization and Phylogeny of U2AF35 Homologs in Plants Plant Physiology, February 1, 2006; 140(2): 624 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lei, I. N. M. Day, and I. Vorechovsky Exonization of AluYa5 in the human ACE gene requires mutations in both 3' and 5' splice sites and is facilitated by a conserved splicing enhancer Nucleic Acids Res., July 14, 2005; 33(12): 3897 - 3906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lutzelberger, E. Backstrom, and G. Akusjarvi Substrate-dependent Differences in U2AF Requirement for Splicing in Adenovirus-infected Cell Extracts J. Biol. Chem., July 8, 2005; 280(27): 25478 - 25484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. HOLLINS, D. A.R. ZORIO, M. MACMORRIS, and T. BLUMENTHAL U2AF binding selects for the high conservation of the C. elegans 3' splice site RNA, March 1, 2005; 11(3): 248 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Webb, S. Lakhe-Reddy, C. M. Romfo, and J. A. Wise Analysis of Mutant Phenotypes and Splicing Defects Demonstrates Functional Collaboration between the Large and Small Subunits of the Essential Splicing Factor U2AF In Vivo Mol. Biol. Cell, February 1, 2005; 16(2): 584 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Webb, C. M. Romfo, W. J. van Heeckeren, and J. A. Wise Exonic splicing enhancers in fission yeast: functional conservation demonstrates an early evolutionary origin Genes & Dev., January 15, 2005; 19(2): 242 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Arrisi-Mercado, M. Romano, A. F. Muro, and F. E. Baralle An Exonic Splicing Enhancer Offsets the Atypical GU-rich 3' Splice Site of Human Apolipoprotein A-II Exon 3 J. Biol. Chem., September 17, 2004; 279(38): 39331 - 39339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nogues, M. J. Munoz, and A. R. Kornblihtt Influence of Polymerase II Processivity on Alternative Splicing Depends on Splice Site Strength J. Biol. Chem., December 26, 2003; 278(52): 52166 - 52171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Kent, A. Reayi, L. Foong, K. A. Chilibeck, and A. M. MacMillan Structuring of the 3' Splice Site by U2AF65 J. Biol. Chem., December 12, 2003; 278(50): 50572 - 50577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Domsic, Y. Wang, A. Mayeda, A. R. Krainer, and C. M. Stoltzfus Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine Tracts Mol. Cell. Biol., December 1, 2003; 23(23): 8762 - 8772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-h. Liang, A. Haritan, S. Uliel, and S. Michaeli trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation Eukaryot. Cell, October 1, 2003; 2(5): 830 - 840. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, J. R. Testaverde, C. N. Davis, W. A. Hayajneh, R. Adair, and A. M. Colberg-Poley Human cytomegalovirus UL37 immediate early target minigene RNAs are accurately spliced and polyadenylated J. Gen. Virol., January 1, 2003; 84(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marani, T. Tenev, D. Hancock, J. Downward, and N. R. Lemoine Identification of Novel Isoforms of the BH3 Domain Protein Bim Which Directly Activate Bax To Trigger Apoptosis Mol. Cell. Biol., June 1, 2002; 22(11): 3577 - 3589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. X. Arredondo-Vega, I. Santisteban, E. Richard, P. Bali, M. Koleilat, M. Loubser, A. Al-Ghonaium, M. Al-Helali, and M. S. Hershfield Adenosine deaminase deficiency with mosaicism for a "second-site suppressor" of a splicing mutation: decline in revertant T lymphocytes during enzyme replacement therapy Blood, February 1, 2002; 99(3): 1005 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shepard, M. Reick, S. Olson, and B. R. Graveley Characterization of U2AF6, a Splicing Factor Related to U2AF35 Mol. Cell. Biol., January 1, 2002; 22(1): 221 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guth, T. O. Tange, E. Kellenberger, and J. Valcarcel Dual Function for U2AF35 in AG-Dependent Pre-mRNA Splicing Mol. Cell. Biol., November 15, 2001; 21(22): 7673 - 7681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Thanaraj and F. Clark Human GC-AG alternative intron isoforms with weak donor sites show enhanced consensus at acceptor exon positions Nucleic Acids Res., June 15, 2001; 29(12): 2581 - 2593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ast, T. Pavelitz, and A. M. Weiner Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction Nucleic Acids Res., April 15, 2001; 29(8): 1741 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Dietrich, M. J. Peris, A. S. Seyboldt, and R. A. Padgett Role of the 3' Splice Site in U12-Dependent Intron Splicing Mol. Cell. Biol., March 15, 2001; 21(6): 1942 - 1952. [Abstract] [Full Text]
|
|
|
|
|
|
L. O. F. Penalva, M. J. Lallena, and J. Valcárcel Switch in 3' Splice Site Recognition between Exon Definition and Splicing Catalysis Is Important for Sex-lethal Autoregulation Mol. Cell. Biol., March 15, 2001; 21(6): 1986 - 1996. [Abstract] [Full Text]
|
|
|
|
|
|
S. Jacquenet, D. Ropers, P. S. Bilodeau, L. Damier, A. Mougin, C. M. Stoltzfus, and C. Branlant Conserved stem-loop structures in the HIV-1 RNA region containing the A3 3' splice site and its cis-regulatory element: possible involvement in RNA splicing Nucleic Acids Res., January 15, 2001; 29(2): 464 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu and A. R. Krainer Pre-mRNA splicing in the absence of an SR protein RS domain Genes & Dev., December 15, 2000; 14(24): 3166 - 3178. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z.-M. Zheng, J. Quintero, E. S. Reid, C. Gocke, and C. C. Baker Optimization of a Weak 3' Splice Site Counteracts the Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor In Vitro and In Vivo J. Virol., July 1, 2000; 74(13): 5902 - 5910. [Abstract] [Full Text]
|
|
|
|
|
|
D. S. McPheeters, B. Schwer, and P. Muhlenkamp Interaction of the yeast DExH-box RNA helicase Prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing Nucleic Acids Res., March 15, 2000; 28(6): 1313 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Gaur, L. Beigelman, P. Haeberli, and T. Maniatis Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing PNAS, January 4, 2000; 97(1): 115 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Alvira, W. F. Goins, J. B. Cohen, and J. C. Glorioso Genetic Studies Exposing the Splicing Events Involved in Herpes Simplex Virus Type 1 Latency-Associated Transcript Production during Lytic and Latent Infection J. Virol., May 1, 1999; 73(5): 3866 - 3876. [Abstract] [Full Text]
|
|
|
|
|
|
J. Southby, C. Gooding, and C. W. J. Smith Polypyrimidine Tract Binding Protein Functions as a Repressor To Regulate Alternative Splicing of alpha -Actinin Mutally Exclusive Exons Mol. Cell. Biol., April 1, 1999; 19(4): 2699 - 2711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chua and R. Reed Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing Genes & Dev., April 1, 1999; 13(7): 841 - 850. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Hodges, R. M. Cripps, M. E. O'Connor, and S. I. Bernstein The Role of Evolutionarily Conserved Sequences in Alternative Splicing at the 3' End of Drosophila melanogaster Myosin Heavy Chain RNA Genetics, January 1, 1999; 151(1): 263 - 276. [Abstract] [Full Text]
|
|
|
|
|
|
O. Gozani, J. Potashkin, and R. Reed A Potential Role for U2AF-SAP 155 Interactions in Recruiting U2 snRNP to the Branch Site Mol. Cell. Biol., August 1, 1998; 18(8): 4752 - 4760. [Abstract] [Full Text]
|
|
|
|
|
|
D. Z. Rudner, K. S. Breger, R. Kanaar, M. D. Adams, and D. C. Rio RNA Binding Activity of Heterodimeric Splicing Factor U2AF: at Least One RS Domain Is Required for High-Affinity Binding Mol. Cell. Biol., July 1, 1998; 18(7): 4004 - 4011. [Abstract] [Full Text]
|
|
|
|
|
|
D. Z. Rudner, R. Kanaar, K. S. Breger, and D. C. Rio Interaction between Subunits of Heterodimeric Splicing Factor U2AF Is Essential In Vivo Mol. Cell. Biol., April 1, 1998; 18(4): 1765 - 1773. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Berglund, N. Abovich, and M. Rosbash A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition Genes & Dev., March 15, 1998; 12(6): 858 - 867. [Abstract] [Full Text]
|
|
|
|
|
|
An Altered Intron Inhibits Synthesis of the Acetylcholine Receptor {alpha}-Subunit in the Paralyzed Zebrafish Mutant nic1 Genetics, January 1, 1998; 148(1): 361 - 372.
|
|
|
|
 |
|
 K. Anderson and M. J. Moore Bimolecular Exon Ligation by the Human Spliceosome Science, June 13, 1997; 276(5319): 1712 - 1716. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Schmauss Enhanced Cleavage of an Atypical Intron of Dopamine D3-Receptor Pre-mRNA in Chronic Schizophrenia J. Neurosci., December 15, 1996; 16(24): 7902 - 7909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J G Umen and C Guthrie A novel role for a U5 snRNP protein in 3' splice site selection. Genes & Dev., April 1, 1995; 9(7): 855 - 868. [Abstract] [PDF]
|
|
|
|
|
|
M Tian and T Maniatis A splicing enhancer exhibits both constitutive and regulated activities. Genes & Dev., July 15, 1994; 8(14): 1703 - 1712. [Abstract] [PDF]
|
|
|
|
|
|
K R Matthews, C Tschudi, and E Ullu A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes. Genes & Dev., February 15, 1994; 8(4): 491 - 501. [Abstract] [PDF]
|
|
|
|
|
|
S Michaud and R Reed A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals. Genes & Dev., June 1, 1993; 7(6): 1008 - 1020. [Abstract] [PDF]
|
|
|
|
|
|
E S Klein, D M Simmons, L W Swanson, and M G Rosenfeld Tissue-specific RNA splicing generates an ankyrin-like domain that affects the dimerization and DNA-binding properties of a bHLH protein. Genes & Dev., January 1, 1993; 7(1): 55 - 71. [Abstract] [PDF]
|
|
|
|
|
|
B E Hoffman and P J Grabowski U1 snRNP targets an essential splicing factor, U2AF65, to the 3' splice site by a network of interactions spanning the exon. Genes & Dev., December 1, 1992; 6(12b): 2554 - 2568. [Abstract] [PDF]
|
|
|
|
|
|
D Frank and C Guthrie An essential splicing factor, SLU7, mediates 3' splice site choice in yeast. Genes & Dev., November 1, 1992; 6(11): 2112 - 2124. [Abstract] [PDF]
|
|
|
|
|
|
J. Steitz Splicing takes a holliday Science, August 14, 1992; 257(5072): 888 - 889. [PDF]
|
|
|
|
|
|
M Tian and T Maniatis Positive control of pre-mRNA splicing in vitro Science, April 10, 1992; 256(5054): 237 - 240. [Abstract] [PDF]
|
|
|
|
|
|
V Goguel, X L Liao, B C Rymond, and M Rosbash U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection. Genes & Dev., August 1, 1991; 5(8): 1430 - 1438. [Abstract] [PDF]
|
|
|
|
|
|
C Guthrie Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein Science, July 12, 1991; 253(5016): 157 - 163. [Abstract] [PDF]
|
|
|
|
|
|
A Gil, P A Sharp, S F Jamison, and M A Garcia-Blanco Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes & Dev., July 1, 1991; 5(7): 1224 - 1236. [Abstract] [PDF]
|
|
|
|
|
|
M P Mullen, C W Smith, J G Patton, and B Nadal-Ginard Alpha-tropomyosin mutually exclusive exon selection: competition between branchpoint/polypyrimidine tracts determines default exon choice. Genes & Dev., April 1, 1991; 5(4): 642 - 655. [Abstract] [PDF]
|
|
|
|
|
|
D L Black Does steric interference between splice sites block the splicing of a short c-src neuron-specific exon in non-neuronal cells? Genes & Dev., March 1, 1991; 5(3): 389 - 402. [Abstract] [PDF]
|
|
|
|
|
|
X D Fu, R A Katz, A M Skalka, and T Maniatis The role of branchpoint and 3'-exon sequences in the control of balanced splicing of avian retrovirus RNA. Genes & Dev., February 1, 1991; 5(2): 211 - 220. [Abstract] [PDF]
|
|
|
|
|
|
H. S. Hummel, R. D. Gillespie, and J. Swindle Mutational Analysis of 3' Splice Site Selection during trans-Splicing J. Biol. Chem., November 3, 2000; 275(45): 35522 - 35531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jacquenet, A. Mereau, P. S. Bilodeau, L. Damier, C. M. Stoltzfus, and C. Branlant A Second Exon Splicing Silencer within Human Immunodeficiency Virus Type 1 tat Exon 2 Represses Splicing of Tat mRNA and Binds Protein hnRNP H J. Biol. Chem., October 26, 2001; 276(44): 40464 - 40475. [Abstract] [Full Text] [PDF]
|
|
