Genes and Development

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GENES & DEVELOPMENT 22:1549-1559, 2008
©2008 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
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Trans-regulation of the expression of the transcription factor MtHAP2-1 by a uORF controls root nodule development

Jean Philippe Combier1,3, Françoise de Billy, Pascal Gamas, Andreas Niebel, and Susana Rivas2

Laboratoire des Interactions Plantes Micro-organismes (LIPM), Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique (CNRS-INRA) UMR 2594/441, F-31320 Castanet Tolosan, France

MtHAP2-1 is a CCAAT-binding transcription factor from the model legume Medicago truncatula. We previously showed that MtHAP2-1 expression is regulated both spatially and temporally by microRNA169. Here we present a novel regulatory mechanism controlling MtHAP2-1 expression. Alternative splicing of an intron in the MtHAP2-1 5'leader sequence (LS) becomes predominant during the development of root nodules, leading to the production of a small peptide, uORF1p. Our results indicate that binding of uORF1p to MtHAP2-1 5'LS mRNA leads to reduced accumulation of the MtHAP2-1 transcript and may contribute to spatial restriction of MtHAP2-1 expression within the nodule. We propose that miR169 and uORF1p play essential, sequential, and nonredundant roles in regulating MtHAP2-1 expression. Importantly, in contrast to previously described cis-acting uORFs, uORF1p is able to act in trans to down-regulate gene expression. Our work thus contributes to a better understanding of the action of upstream ORFs (uORFs) in the regulation of gene expression.

[Keywords: HAP2; Medicago truncatula; alternative splicing; symbiosis; transcription factor; upstream ORF]

Received October 29, 2007; revised version accepted April 8, 2008.

1 Present address: Institut National de la Santé et de la Recherche Médicale (INSERM) U563, Department Lipoprotéines et Médiateurs Lipidiques, Hôpital Purpan, 31024 Toulouse, France

2 Corresponding authors.

E-MAIL susana.rivas{at}toulouse.inra.fr; FAX 33-5-61285061.

3 E-MAIL jpcombier{at}yahoo.fr; FAX 33-5-61779412.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.461808.


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