QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mitchell, J. A. Articles by Fraser, P. Search for Related Content PubMed PubMed Citation Articles by Mitchell, J. A. Articles by Fraser, P. Social Bookmarking                   What's this?

RESEARCH COMMUNICATION

Transcription factories are nuclear subcompartments that remain in the absence of transcription

Laboratory of Chromatin and Gene Expression, The Babraham Institute, Cambridge CB22 3AT, United Kingdom

Nascent transcription occurs at nuclear foci of concentrated, hyperphosphorylated RNA polymerase II (RNAPII). We investigate RNAPII localization, distal gene coassociation, and Hbb locus conformation during inhibition of transcription. Our results show distal active genes remain associated with RNAPII foci and each other in the absence of elongation. When initiation is inhibited, active genes dissociate from RNAPII foci and each other, suggesting initiation is necessary to tether distal active genes to shared foci. In the absence of transcription RNAPII foci remain, indicating they are not simple accumulations of RNAPII on transcribed genes but exist as independent nuclear subcompartments.

[Keywords: RNA polymerase II; nuclear organization; fluorescence in situ hybridization; locus control region; chromosome conformation capture]]

Received August 23, 2007; revised version accepted November 1, 2007.

E-MAIL Peter.Fraser{at}bbsrc.ac.uk; FAX 44-1223-496022.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.454008

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Xu and P. R. Cook Similar active genes cluster in specialized transcription factories J. Cell Biol., May 19, 2008; 181(4): 615 - 623. [Abstract] [Full Text] [PDF]