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RESEARCH COMMUNICATION

MicroRNAs in the Drosophila bithorax complex

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA

The iab-4 noncoding RNA from the Drosophila bithorax complex is the substrate for a microRNA (miRNA). Gene conversion was used to delete the hairpin precursor of this miRNA; flies homozygous for this deletion are sterile. Surprisingly, this mutation complements with rearrangement breakpoint mutations that disrupt the iab-4 RNA but fails to complement with breaks mapping in the iab-5 through iab-7 regulatory regions. These breaks disrupt the iab-8 RNA, transcribed from the opposite strand. This iab-8 RNA also encodes a miRNA, detected on Northern blots, derived from the hairpin complementary to the iab-4 precursor hairpin. Ultrabithorax is a target of both miRNAs, although its repression is subtle in both cases.

[Keywords: Ultrabithorax; noncoding RNA; abdominal-A; iab-8 RNA]]

Received September 10, 2007; revised version accepted November 9, 2007.

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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1614208

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