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RESEARCH COMMUNICATION

A novel checkpoint mechanism regulating the G1/S transition

¹ Department of Cell Biology, Rikshospitalet-Radiumhospitalet Medical Centre and University of Oslo, Montebello, 0310 Oslo, Norway; ² Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom

Ultraviolet irradiation of fission yeast cells in G1 phase induced a delay in chromatin binding of replication initiation factors and, consistently, a transient delay in S-phase entry. The cell cycle delay was totally dependent on the Gcn2 kinase, a sensor of the nutritional status, and was accompanied by phosphorylation of the translation initiation factor eIF2 and by a general depression of translation. However, the G1-specific synthesis of factors required for DNA replication was not reduced by ultraviolet radiation. The cell cycle delay represents a novel checkpoint with a novel mechanism of action that is not activated by ionizing radiation.

[Keywords: Cell cycle; checkpoint; DNA replication; translation; UV irradiation]

Received December 11, 2006; revised version accepted January 31, 2007.

E-MAIL eboye@rr-research.no; FAX 47-22934580.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.421807

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