QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hogg, J. R. Articles by Collins, K. Search for Related Content PubMed PubMed Citation Articles by Hogg, J. R. Articles by Collins, K. Social Bookmarking                   What's this?

RESEARCH COMMUNICATION

Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control

Department of Molecular and Cell Biology, University of California at Berkely, Berkeley, California 94720 USA

Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5–5S RNPs establish 5S RNA as a target of quality control.

[Keywords: RNA Affinity in Tandem (RAT); noncoding RNA; quality control surveillance]]

Received August 13, 2007; revised version accepted October 11, 2007.

² Corresponding authors.

E-MAIL kcollins{at}berkeley.edu; FAX (510) 643-6334.

³ E-MAIL jh2721{at}columbia.edu; FAX .

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1603907

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?