QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data All Versions of this Article: gad.1608807v1 21/22/2936    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Isogai, Y. Articles by Tjian, R. Search for Related Content PubMed PubMed Citation Articles by Isogai, Y. Articles by Tjian, R. Related Content Related Article Social Bookmarking                   What's this?

Transcription of histone gene cluster by differential core-promoter factors

¹ Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA; ² Department of Statistics, Department of Biostatistics, and Department of Medical Informatics, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA; ³ Adolf-Butenandt-Institut, Molekularbiologie, 80336 Munich, Germany; ⁴ Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, California 94720, USA; ⁵ Li Ka-Shing Center for Biomedical and Health Sciences, University of California at Berkeley, Berkeley, California 94720, USA

The 100 copies of tandemly arrayed Drosophila linker (H1) and core (H2A/B and H3/H4) histone gene cluster are coordinately regulated during the cell cycle. However, the molecular mechanisms that must allow differential transcription of linker versus core histones prevalent during development remain elusive. Here, we used fluorescence imaging, biochemistry, and genetics to show that TBP (TATA-box-binding protein)-related factor 2 (TRF2) selectively regulates the TATA-less Histone H1 gene promoter, while TBP/TFIID targets core histone transcription. Importantly, TRF2-depleted polytene chromosomes display severe chromosomal structural defects. This selective usage of TRF2 and TBP provides a novel mechanism to differentially direct transcription within the histone cluster. Moreover, genome-wide chromatin immunoprecipitation (ChIP)-on-chip analyses coupled with RNA interference (RNAi)-mediated functional studies revealed that TRF2 targets several classes of TATA-less promoters of >1000 genes including those driving transcription of essential chromatin organization and protein synthesis genes. Our studies establish that TRF2 promoter recognition complexes play a significantly more central role in governing metazoan transcription than previously appreciated.

[Keywords: TATA-box-binding protein (TBP); TBP-related factor 2 (TRF2); core-promoter recognition; histone gene cluster; ChIP-on-chip; chromatin organization]]

Received August 24, 2007; revised version accepted September 21, 2007.

E-MAIL jmlim{at}berkeley.edu; FAX (510) 643-9547.

Supplemental material is available at http://www.genesdev.org.

Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.1608807

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

On a roll for new TRF targets

Jaime H. Reina and Nouria Hernandez
Genes & Dev. 2007 21: 2855-2860. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				J.-Y. Hsu, T. Juven-Gershon, M. T. Marr II, K. J. Wright, R. Tjian, and J. T. Kadonaga TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription Genes & Dev., September 1, 2008; 22(17): 2353 - 2358. [Abstract] [Full Text] [PDF]

				J. H. Reina and N. Hernandez On a roll for new TRF targets Genes & Dev., November 15, 2007; 21(22): 2855 - 2860. [Full Text] [PDF]