QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data All Versions of this Article: gad.1583407v1 21/17/2137    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deato, M. D. E. Articles by Tjian, R. Search for Related Content PubMed PubMed Citation Articles by Deato, M. D. E. Articles by Tjian, R. Related Content Related Article Social Bookmarking                   What's this?

Switching of the core transcription machinery during myogenesis

Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA

Transcriptional mechanisms that govern cellular differentiation typically include sequence-specific DNA-binding proteins and chromatin-modifying activities. These regulatory factors are assumed necessary and sufficient to drive both divergent programs of proliferation and terminal differentiation. By contrast, potential contributions of the basal transcriptional apparatus to orchestrate cell-specific gene expression have been poorly explored. In order to probe alternative mechanisms that control differentiation, we have assessed the fate of the core promoter recognition complex, TFIID, during skeletal myogenesis. Here we report that differentiation of myoblast to myotubes involves the disruption of the canonical holo-TFIID and replacement by a novel TRF3/TAF3 (TBP-related factor 3/TATA-binding protein-associated factor 3) complex. This required switching of core promoter complexes provides organisms a simple yet effective means to selectively turn on one transcriptional program while silencing many others. Although this drastic but parsimonious transcriptional switch had previously escaped our attention, it may represent a more general mechanism for regulating cell type-specific terminal differentiation.

[Keywords: TFIID; TAF3; TRF3; differentiation; myogenesis]

Received June 14, 2007; revised version accepted July 13, 2007.

E-MAIL jmlim{at}berkeley.edu; FAX (510) 643-9547.

Supplemental material is available at http://www.genesdev.org.

Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.1583407

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

Transcription strategies in terminally differentiated cells: shaken to the core

Katherine A. Jones
Genes & Dev. 2007 21: 2113-2117. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				J.-Y. Hsu, T. Juven-Gershon, M. T. Marr II, K. J. Wright, R. Tjian, and J. T. Kadonaga TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription Genes & Dev., September 1, 2008; 22(17): 2353 - 2358. [Abstract] [Full Text] [PDF]

				J. H. Reina and N. Hernandez On a roll for new TRF targets Genes & Dev., November 15, 2007; 21(22): 2855 - 2860. [Full Text] [PDF]