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RESEARCH COMMUNICATION

MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula

¹ LIPM (Laboratoire des Interactions Plantes-Microorganismes) INRA-CNRS (Institut de National de la Recherche-Centre national de la recherche scientifique), 31326 Castanet-Tolosan, France; ² ISV (Institut des Sciences du Végétal), CNRS, 91198 Gif sur Yvette, France

In the model legume Medicago truncatula, we identified a new transcription factor of the CCAAT-binding family, MtHAP2-1, for which RNA interference (RNAi) and in situ hybridization experiments indicate a key role during nodule development, possibly by controlling nodule meristem function. We could also show that MtHAP2-1 is regulated by microRNA169, whose overexpression leads to the same nodule developmental block as MtHAP2-1 RNAi constructs. The complementary expression pattern of miR169 and MtHAP2-1 and the phenotype of miR169-resistant MtHAP2-1 nodules strongly suggest, in addition, that the miR169-mediated restriction of MtHAP2-1 expression to the nodule meristematic zone is essential for the differentiation of nodule cells.

[Keywords: Nodulation; microRNA; transcription factor; symbiosis; HAP2; Medicago]

Received July 24, 2006; revised version accepted September 25, 2006.

E-MAIL aniebel{at}toulouse.inra.fr; FAX 33-5-61285061.

⁴ E-MAIL martin.crespi{at}isv.cnrs-gif.fr; FAX 33-1-69823695.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.402806

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