QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Reseach Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grallert, A. Articles by Hagan, I. M. Search for Related Content PubMed PubMed Citation Articles by Grallert, A. Articles by Hagan, I. M. Social Bookmarking                   What's this?

S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner

¹ Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, Manchester M20 4BX, United Kingdom; ² Heinrich-Heine-Universität, Lehrstuhl für funktionelle Genomforschung der Mikroorganismen, 40225 Düsseldorf, Germany; ³ Advanced Imaging Facility, Paterson Institute for Cancer Research, Manchester M20 4BX, United Kingdom

The Schizosaccharomyces pombe CLIP170-associated protein (CLASP) Peg1 was identified in a screen for mutants with spindle formation defects and a screen for molecules that antagonized EB1 function. The conditional peg1.1 mutant enabled us to identify key features of Peg1 function. First, Peg1 was required to form a spindle and astral microtubules, yet destabilized interphase microtubules. Second, Peg1 was required to slow the polymerization rate of interphase microtubules that establish end-on contact with the cortex at cell tips. Third, Peg1 antagonized the action of S. pombe CLIP170 (Tip1) and EB1 (Mal3). Fourth, although Peg1 resembled higher eukaryotic CLASPs by physically associating with both Mal3 and Tip1, neither Tip1 nor Mal3 was required for Peg1 to destabilize interphase microtubules or for it to associate with microtubules. Conversely, neither Mal3 nor Tip1 required Peg1 to associate with microtubules or cell tips. Consistently, while mal3. and tip1. disrupted linear growth, corrupting peg1 ⁺ did not. Fifth, peg1.1 phenotypes resembled those arising from deletion of the single heavy or both light chains of fission yeast dynein. Furthermore, all interphase phenotypes arising from peg1 ⁺ manipulation relied on dynein function. Thus, the impact of S. pombe CLASP on interphase microtubule behavior is more closely aligned to dynein than EB1 or CLIP170.

[Keywords: CLASP; dynein; S. pombe; fission yeast; CLIP170; EB1]

Received January 24, 2006; revised version accepted July 6, 2006.

⁵ Corresponding author. E-MAIL ihagan{at}picr.man.ac.uk; FAX 44-161-446-3109.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.381306.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Chiron, A. Bobkova, H. Zhou, and M. P. Yaffe CLASP regulates mitochondrial distribution in Schizosaccharomyces pombe J. Cell Biol., July 14, 2008; 182(1): 41 - 49. [Abstract] [Full Text] [PDF]

				R. R. Daga and P. Nurse Interphase microtubule bundles use global cell shape to guide spindle alignment in fission yeast J. Cell Sci., June 15, 2008; 121(12): 1973 - 1980. [Abstract] [Full Text] [PDF]

				V. Kirik, U. Herrmann, C. Parupalli, J. C. Sedbrook, D. W. Ehrhardt, and M. Hulskamp CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis J. Cell Sci., December 15, 2007; 120(24): 4416 - 4425. [Abstract] [Full Text] [PDF]

				A. Grallert, R. Martin-Garcia, S. Bagley, and D. P. Mulvihill In vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain J. Cell Sci., December 1, 2007; 120(23): 4093 - 4098. [Abstract] [Full Text] [PDF]

				I. Alvarez-Tabares, A. Grallert, J.-M. Ortiz, and I. M. Hagan Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth J. Cell Sci., October 15, 2007; 120(20): 3589 - 3601. [Abstract] [Full Text] [PDF]

				A. Kerres, V. Jakopec, and U. Fleig The Conserved Spc7 Protein Is Required for Spindle Integrity and Links Kinetochore Complexes in Fission Yeast Mol. Biol. Cell, July 1, 2007; 18(7): 2441 - 2454. [Abstract] [Full Text] [PDF]