QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Homa, F L Articles by Levine, M Search for Related Content PubMed PubMed Citation Articles by Homa, F L Articles by Levine, M Social Bookmarking                   What's this?

Research Papers

A specific 15-bp TATA box promoter element is required for expression of a herpes simplex virus type 1 late gene.

Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.

Abstract

The herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) gene is a true late or gamma 2 gene in that its expression shows a strict requirement for viral DNA replication. Elements required for regulated expression of this gene were previously shown to consist of the gC TATA box, transcription start site and a large portion of the leader sequence of the gC gene. In this paper we show that transcription of the gC gene requires a 15-bp sequence, GGGTATAAATTCCGG, which contains the gC TATA box. This sequence contains specific promoter elements because replacement of this sequence with either the TATA box of the HSV-1 early thymidine kinase (tk) gene or two random TATA-like elements results in a transcriptionally inactive gC gene. In addition, we show that temporal expression of HSV beta and gamma genes at early and late times during infection are controlled by separate and distinct regulatory elements; regulatory signals distal to the TATA box are needed for early expression, whereas a gC-like TATA box is needed for late expression. These signals were identified by construction of a chimeric HSV gene that contained the distal control signals of the beta tk gene fused upstream of the TATA sequence of the gamma 2 gC gene. When RNA was isolated at various times postinfection from cells infected with a virus whose genome contained this chimeric tk-gC gene, synthesis of gC mRNA showed both early and late kinetics.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Isomura, M. F. Stinski, A. Kudoh, T. Murata, S. Nakayama, Y. Sato, S. Iwahori, and T. Tsurumi Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts J. Virol., February 15, 2008; 82(4): 1638 - 1646. [Abstract] [Full Text] [PDF]

				S. Tang, K. Yamanegi, and Z.-M. Zheng Requirement of a 12-Base-Pair TATT-Containing Sequence and Viral Lytic DNA Replication in Activation of the Kaposi's Sarcoma-Associated Herpesvirus K8.1 Late Promoter J. Virol., March 1, 2004; 78(5): 2609 - 2614. [Abstract] [Full Text] [PDF]

				J. W. Bruce and K. W. Wilcox Identification of a Motif in the C Terminus of Herpes Simplex Virus Regulatory Protein ICP4 That Contributes to Activation of Transcription J. Virol., January 1, 2002; 76(1): 195 - 207. [Abstract] [Full Text] [PDF]

				J. Chang and D. Ganem On the control of late gene expression in Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) J. Gen. Virol., August 1, 2000; 81(8): 2039 - 2047. [Abstract] [Full Text]

				T. R. Serio, N. Cahill, M. E. Prout, and G. Miller A Functionally Distinct TATA Box Required for Late Progression through the Epstein-Barr Virus Life Cycle J. Virol., October 1, 1998; 72(10): 8338 - 8343. [Abstract] [Full Text] [PDF]

				D. Martin and P. Weber Role of the DR2 repeat array in the regulation of the ICP34.5 gene promoter of herpes simplex virus type 1 during productive infection J. Gen. Virol., March 1, 1998; 79(3): 517 - 523. [Abstract]

				S. Huang and L. A. Hanson Temporal Gene Regulation of the Channel Catfish Virus (Ictalurid Herpesvirus 1) J. Virol., March 1, 1998; 72(3): 1910 - 1917. [Abstract] [Full Text] [PDF]

				E. Gonzalez-Couto, N. Klages, and M. Strubin Synergistic and promoter-selective activation of transcription by recruitment of transcription factors TFIID and TFIIB PNAS, July 22, 1997; 94(15): 8036 - 8041. [Abstract] [Full Text] [PDF]

				M A Collart and K Struhl NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Genes & Dev., March 1, 1994; 8(5): 525 - 537. [Abstract] [PDF]

				S Oliviero, G S Robinson, K Struhl, and B M Spiegelman Yeast GCN4 as a probe for oncogenesis by AP-1 transcription factors: transcriptional activation through AP-1 sites is not sufficient for cellular transformation. Genes & Dev., September 1, 1992; 6(9): 1799 - 1809. [Abstract] [PDF]

				A McCormick, H Brady, J Fukushima, and M Karin The pituitary-specific regulatory gene GHF1 contains a minimal cell type-specific promoter centered around its TATA box. Genes & Dev., August 1, 1991; 5(8): 1490 - 1503. [Abstract] [PDF]