QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vasyutina, E. Articles by Birchmeier, C. Search for Related Content PubMed PubMed Citation Articles by Vasyutina, E. Articles by Birchmeier, C. Social Bookmarking                   What's this?

RESEARCH PAPER

CXCR4 and Gab1 cooperate to control the development of migrating muscle progenitor cells

¹ Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany; ² Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany; ³ Institute of Anatomy and Cell Biology, University of Freiburg, 79104 Freiburg, Germany; ⁴ Department of Pharmacology and Toxicology, Otto-von-Guericke University, 39120 Magdeburg, Germany.

Long-range migrating progenitor cells generate hypaxial muscle, for instance the muscle of the limbs, hypoglossal cord, and diaphragm. We show here that migrating muscle progenitors express the chemokine receptor CXCR4. The corresponding ligand, SDF1, is expressed in limb and branchial arch mesenchyme; i.e., along the routes and at the targets of the migratory cells. Ectopic application of SDF1 in the chick limb attracts muscle progenitor cells. In CXCR4 mutant mice, the number of muscle progenitors that colonize the anlage of the tongue and the dorsal limb was reduced. Changes in the distribution of the muscle progenitor cells were accompanied by increased apoptosis, indicating that CXCR4 signals provide not only attractive cues but also control survival. Gab1 encodes an adaptor protein that transduces signals elicited by tyrosine kinase receptors, for instance the c-Met receptor, and plays a role in the migration of muscle progenitor cells. We found that CXCR4 and Gab1 interact genetically. For instance, muscle progenitors do not reach the anlage of the tongue in CXCR4;Gab1 double mutants; this target is colonized in either of the single mutants. Our analysis reveals a role of SDF1/CXCR4 signaling in the development of migrating muscle progenitors and shows that a threshold number of progenitor cells is required to generate muscle of appropriate size.

[Keywords: CXCR4; Gab1; hypaxial muscle; migration]

Received April 7, 2005; revised version accepted July 13, 2005.

Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.346205.

⁵ Corresponding author.
E-MAIL cbirch{at}mdc-berlin.de; FAX 49-30-9406-3765.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Yahiaoui, D. Gvozdic, G. Danialou, M. Mack, and B. J. Petrof CC family chemokines directly regulate myoblast responses to skeletal muscle injury J. Physiol., August 15, 2008; 586(16): 3991 - 4004. [Abstract] [Full Text] [PDF]

				G.-U. Bae, U. Gaio, Y.-J. Yang, H.-J. Lee, J.-S. Kang, and R. S. Krauss Regulation of Myoblast Motility and Fusion by the CXCR4-associated Sialomucin, CD164 J. Biol. Chem., March 28, 2008; 283(13): 8301 - 8309. [Abstract] [Full Text] [PDF]

				G. Junion, L. Bataille, T. Jagla, J. P. Da Ponte, R. Tapin, and K. Jagla Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors Genes & Dev., December 1, 2007; 21(23): 3163 - 3180. [Abstract] [Full Text] [PDF]

				V. Odemis, K. Boosmann, M. T. Dieterlen, and J. Engele The chemokine SDF1 controls multiple steps of myogenesis through atypical PKC{zeta} J. Cell Sci., November 15, 2007; 120(22): 4050 - 4059. [Abstract] [Full Text] [PDF]

				U. Schaeper, R. Vogel, J. Chmielowiec, J. Huelsken, M. Rosario, and W. Birchmeier Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo PNAS, September 25, 2007; 104(39): 15376 - 15381. [Abstract] [Full Text] [PDF]

				S. Tozer, M.-A. Bonnin, F. Relaix, S. Di Savino, P. Garcia-Villalba, P. Coumailleau, and D. Duprez Involvement of vessels and PDGFB in muscle splitting during chick limb development Development, July 15, 2007; 134(14): 2579 - 2591. [Abstract] [Full Text] [PDF]

				M. A. Sieber, R. Storm, M. Martinez-de-la-Torre, T. Muller, H. Wende, K. Reuter, E. Vasyutina, and C. Birchmeier Lbx1 Acts as a Selector Gene in the Fate Determination of Somatosensory and Viscerosensory Relay Neurons in the Hindbrain J. Neurosci., May 2, 2007; 27(18): 4902 - 4909. [Abstract] [Full Text] [PDF]

				E. Vasyutina, D. C. Lenhard, H. Wende, B. Erdmann, J. A. Epstein, and C. Birchmeier RBP-J (Rbpsuh) is essential to maintain muscle progenitor cells and to generate satellite cells PNAS, March 13, 2007; 104(11): 4443 - 4448. [Abstract] [Full Text] [PDF]

				P. Giacobini, A. Messina, S. Wray, C. Giampietro, T. Crepaldi, P. Carmeliet, and A. Fasolo Hepatocyte Growth Factor Acts as a Motogen and Guidance Signal for Gonadotropin Hormone-Releasing Hormone-1 Neuronal Migration J. Neurosci., January 10, 2007; 27(2): 431 - 445. [Abstract] [Full Text] [PDF]