![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RESEARCH PAPER
Department of Genetics, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
Previously, we identified multiple in vivo mRNA targets of the maxi-KH/STAR domain protein GLD-1 by their ability to interact with GLD-1 in cytoplasmic extracts and, for all targets tested thus far, GLD-1 functions as a translational repressor. However, here we show that GLD-1 stabilizes the mRNAs of two targets, gna-2 (T23G11.2) and Y75B12B.1. gna-2 mRNA has two upstream open reading frames (uORF), resulting in two premature stop codons. We found that gna-2 mRNA is a naturally occurring mRNA target of nonsense-mediated mRNA decay (NMD) and that the binding of GLD-1 protects gna-2 mRNA from NMD, likely by repressing translation of the uORFs. Therefore, gna-2 mRNA comes under two posttranscriptional controls: (1) translation regulation by a specific translational repressor, GLD-1; and (2) uORF elicited regulation, mainly through NMD. As a result, these two posttranscriptional controls together provide precise temporal and spatial control of gene expression. Consistent with this novel mode of regulation, when GLD-1 mRNA targets acquire premature stop codon mutations, GLD-1 protects them from NMD. Analysis of several mRNA targets containing premature stop codons suggests that in translation repression, GLD-1 either represses ribosome assembly on the target mRNA, or subsequent ribosome elongation to the premature stop codon.
[Keywords: Germ line; translation repression; upstream open reading frame; nonsense-mediated mRNA decay; gld-1; gna-2]
Received January 22, 2004; revised version accepted March 23, 2004.
Supplemental material is available at http://www.genesdev.org.
1 Corresponding author.
E-MAIL ts{at}genetics.wustl.edu; FAX (314) 362-7875.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Ohno, M. Hagiwara, and H. Kuroyanagi STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo Genes & Dev., February 1, 2008; 22(3): 360 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Lee, M. Ohmachi, S. Arur, S. Nayak, R. Francis, D. Church, E. Lambie, and T. Schedl Multiple Functions and Dynamic Activation of MPK-1 Extracellular Signal-Regulated Kinase Signaling in Caenorhabditis elegans Germline Development Genetics, December 1, 2007; 177(4): 2039 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fire, R. Alcazar, and F. Tan Unusual DNA Structures Associated With Germline Genetic Activity in Caenorhabditis elegans Genetics, July 1, 2006; 173(3): 1259 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Olson, J. R. Bishop, J. R. Yates, K. Oegema, and J. D. Esko Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2 J. Cell Biol., June 19, 2006; 173(6): 985 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rosenstiel, K. Huse, A. Till, J. Hampe, S. Hellmig, C. Sina, S. Billmann, O. von Kampen, G. H. Waetzig, M. Platzer, et al. A short isoform of NOD2/CARD15, NOD2-S, is an endogenous inhibitor of NOD2/receptor-interacting protein kinase 2-induced signaling pathways PNAS, February 28, 2006; 103(9): 3280 - 3285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Vought, M. Ohmachi, M.-H. Lee, and E. M. Maine EGO-1, a Putative RNA-Directed RNA Polymerase, Promotes Germline Proliferation in Parallel With GLP-1/Notch Signaling and Regulates the Spatial Organization of Nuclear Pore Complexes and Germline P Granules in Caenorhabditis elegans Genetics, July 1, 2005; 170(3): 1121 - 1132. [Abstract] [Full Text] [PDF]
|
|
