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RESEARCH COMMUNICATION

A role for -actin in RNA polymerase III transcription

¹ Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA; ² Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated -actin, and -actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated -actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of -actin. This suggests that -actin associated with pol III is essential for basal pol III transcription.

[Keywords: RNA polymerase III; -actin; human U6 snRNA promoter; CK2]

Received August 27, 2004; revised version accepted October 8, 2004.

Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1250804.

³ Corresponding author.
E-MAIL Hernande{at}cshl.edu; FAX (516) 367-6801.

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