![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
1 Medical Research Council Laboratory for Molecular Cell Biology, Cell Biology Unit, and the Biology Department, University College London, London WC1E 6BT, United Kingdom; 2 Centre for Brain Repair, University of Cambridge, Cambridge CB2 2PY, United Kingdom
We showed previously that purified rat oligodendrocyte precursor cells (OPCs) can be induced by extracellular signals to convert to multipotent neural stem-like cells (NSLCs), which can then generate both neurons and glial cells. Because the conversion of precursor cells to stem-like cells is of both intellectual and practical interest, it is important to understand its molecular basis. We show here that the conversion of OPCs to NSLCs depends on the reactivation of the sox2 gene, which in turn depends on the recruitment of the tumor suppressor protein Brca1 and the chromatin-remodeling protein Brahma (Brm) to an enhancer in the sox2 promoter. Moreover, we show that the conversion is associated with the modification of Lys 4 and Lys 9 of histone H3 at the same enhancer. Our findings suggest that the conversion of OPCs to NSLCs depends on progressive chromatin remodeling, mediated in part by Brca1 and Brm.
[Keywords: Oligodendrocyte precursor cells; neural stem cells; Sox2; Brca1; Brm; histone modification]
Received May 17, 2004; revised version accepted September 21, 2004.
3 Corresponding author.
E-MAIL tk294{at}cam.ac.uk; FAX 44-1223-334121.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Fong, K. A. Hohenstein, and P. J. Donovan Regulation of Self-Renewal and Pluripotency by Sox2 in Human Embryonic Stem Cells Stem Cells, August 1, 2008; 26(8): 1931 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, L. Shi, L. Zhang, R. Li, J. Liang, W. Yu, L. Sun, X. Yang, Y. Wang, Y. Zhang, et al. The Molecular Mechanism Governing the Oncogenic Potential of SOX2 in Breast Cancer J. Biol. Chem., June 27, 2008; 283(26): 17969 - 17978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, C. A. Ghiani, J. Y. Kim, A. Liu, J. Sandoval, J. DeVellis, and P. Casaccia-Bonnefil Inhibition of p53 Transcriptional Activity: A Potential Target for Future Development of Therapeutic Strategies for Primary Demyelination J. Neurosci., June 11, 2008; 28(24): 6118 - 6127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. V. Lunyak and M. G. Rosenfeld Epigenetic regulation of stem cell fate Hum. Mol. Genet., April 15, 2008; 17(R1): R28 - R36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cavallaro, J. Mariani, C. Lancini, E. Latorre, R. Caccia, F. Gullo, M. Valotta, S. DeBiasi, L. Spinardi, A. Ronchi, et al. Impaired generation of mature neurons by neural stem cells from hypomorphic Sox2 mutants Development, February 1, 2008; 135(3): 541 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Das, J. James, S. Bhattacharya, A. N. Imbalzano, M. L. Antony, G. Hegde, X. Zhao, K. Mallya, F. Ahmad, E. Knudsen, et al. SWI/SNF Chromatin Remodeling ATPase Brm Regulates the Differentiation of Early Retinal Stem Cells/Progenitors by Influencing Brn3b Expression and Notch Signaling J. Biol. Chem., November 30, 2007; 282(48): 35187 - 35201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Lyssiotis, J. Walker, C. Wu, T. Kondo, P. G. Schultz, and X. Wu Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells PNAS, September 18, 2007; 104(38): 14982 - 14987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liu, Y. R. Han, J. Li, D. Sun, M. Ouyang, M. R. Plummer, and P. Casaccia-Bonnefil The Glial or Neuronal Fate Choice of Oligodendrocyte Progenitors Is Modulated by Their Ability to Acquire an Epigenetic Memory J. Neurosci., July 4, 2007; 27(27): 7339 - 7343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kulbatski, A. J. Mothe, A. Keating, Y. Hakamata, E. Kobayashi, and C. H. Tator Oligodendrocytes and Radial Glia Derived From Adult Rat Spinal Cord Progenitors: Morphological and Immunocytochemical Characterization J. Histochem. Cytochem., March 1, 2007; 55(3): 209 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pellegatta, P. L. Poliani, D. Corno, F. Menghi, F. Ghielmetti, B. Suarez-Merino, V. Caldera, S. Nava, M. Ravanini, F. Facchetti, et al. Neurospheres Enriched in Cancer Stem-Like Cells Are Highly Effective in Eliciting a Dendritic Cell-Mediated Immune Response against Malignant Gliomas Cancer Res., November 1, 2006; 66(21): 10247 - 10252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Y. Sun and A. J. D'Ercole Insulin-Like Growth Factor-I Stimulates Histone H3 and H4 Acetylation in the Brain in Vivo Endocrinology, November 1, 2006; 147(11): 5480 - 5490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Diks, R. J. Bink, S. van de Water, J. Joore, C. van Rooijen, F. J. Verbeek, J. den Hertog, M. P. Peppelenbosch, and D. Zivkovic The novel gene asb11: a regulator of the size of the neural progenitor compartment J. Cell Biol., August 14, 2006; 174(4): 581 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miyagi, M. Nishimoto, T. Saito, M. Ninomiya, K. Sawamoto, H. Okano, M. Muramatsu, H. Oguro, A. Iwama, and A. Okuda The Sox2 Regulatory Region 2 Functions as a Neural Stem Cell-specific Enhancer in the Telencephalon J. Biol. Chem., May 12, 2006; 281(19): 13374 - 13381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Taranova, S. T. Magness, B. M. Fagan, Y. Wu, N. Surzenko, S. R. Hutton, and L. H. Pevny SOX2 is a dose-dependent regulator of retinal neural progenitor competence. Genes & Dev., May 1, 2006; 20(9): 1187 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Puente, D. J. Borris, J.-F. Carriere, J. F. Kelly, and L. A. Megeney Identification of Candidate Regulators of Embryonic Stem Cell Differentiation by Comparative Phosphoprotein Affinity Profiling Mol. Cell. Proteomics, January 1, 2006; 5(1): 57 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. W. Rowe, D. M. Jeftinija, K. Jeftinija, and S. Jeftinija Development of Functional Neurons from Postnatal Stem Cells In Vitro Stem Cells, September 1, 2005; 23(8): 1044 - 1049. [Abstract] [Full Text] [PDF]
|
|
