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RESEARCH COMMUNICATION

siRNAs targeting an intronic transposon in the regulation of natural flowering behavior in Arabidopsis

¹ Waksman Institute, Rutgers University, Piscataway, New Jersey 08854, USA; ² Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA

Allelic variation in FLOWERING LOCUS C (FLC), a central repressor of flowering, contributes to natural differences in flowering behavior among Arabidopsis accessions. The weak nature of the FLC allele in the Ler accession is due to low levels of FLC RNA resulting, through an unknown mechanism, from a transposable element inserted in an intron of FLC. Here we show that the transposable element renders FLC-Ler subject to repressive chromatin modifications mediated by short interfering RNAs generated from homologous transposable elements in the genome. Our studies have general implications for the role of transposable elements in eukaryotic gene expression and evolution.

[Keywords: Transposon; siRNA; intron; FLC; HEN1; H3-K9 methylation]

Received May 3, 2004; revised version accepted September 27, 2004.

Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1217304.

³ Present address: School of Life Science, Shanghai University, Shangda Road 99, Shanghai 200436, P.R. China.

⁴ Corresponding author.
E-MAIL xuemei{at}waksman.rutgers.edu; FAX (732) 445-5735.

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