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REVIEW
1 Howard Hughes Medical Institute and 2 Division of Nucleic Acids Enzymology, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elongation factors that modulate the activity of RNAP II, and the more recently identified factors that facilitate elongation on chromatin templates. Additionally, we discuss the factors that associate with RNAP II, but do not modulate its catalytic activity. Elongation is highlighted as a central process that coordinates multiple stages in mRNA biogenesis and maturation.
[Keywords: Elongation; transcription; RNA polymerase II; chromatin; CTD]
3 Corresponding author. E-MAIL reinbedf{at}UMDNJ.EDU; FAX (732) 235-5294.
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 Q. Zhou and J. H. N. Yik The Yin and Yang of P-TEFb Regulation: Implications for Human Immunodeficiency Virus Gene Expression and Global Control of Cell Growth and Differentiation Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 646 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
 |
|
 J. Ausio Histone variants--the structure behind the function Brief Funct Genomic Proteomic, September 1, 2006; 5(3): 228 - 243. [Abstract] [Full Text] [PDF]
|
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|
|
|
|
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|
|
|
|
|
|
D. Reinberg and R. J. Sims III de FACTo Nucleosome Dynamics J. Biol. Chem., August 18, 2006; 281(33): 23297 - 23301. [Abstract] [Full Text] [PDF]
|
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|
|
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