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RESEARCH PAPER
Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
In mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.
[Keywords: Gene expression; splicing; translation; polysome; exon junction complex]
Received October 21, 2003; revised version accepted December 10, 2003.
1 Present address: Centre de Génétique Moléculaire, C.N.R.S., Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
2 Corresponding author. E-MAIL mmoore{at}brandeis.edu; FAX (781) 736-2337.
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