.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
Gene amplification at the chorion loci in Drosophila ovarian follicle cells is a model for the developmental regulation of DNA replication. Previously, we showed that the Drosophila homolog of the Myb oncoprotein family (DmMyb) is tightly associated with four additional proteins and that DmMyb is required for this replication-mediated amplification. Here we used targeted mutagenesis to generate a mutant in the largest subunit of the DmMyb complex, the Aly and Lin-9 family member, Myb-interacting protein 130 (Mip130). We found that mip130 mutant females are sterile and display inappropriate bromodeoxyuridine (BrdU) incorporation throughout the follicle cell nuclei at stages undergoing gene amplification. Whereas mutations in Dm-myb are lethal, mutations in mip130 are viable. Surprisingly, Dm-myb mip130 double mutants are also viable and display the same phenotypes as mip130 mutants alone. This suggests that Mip130 activity without DmMyb counteraction may be responsible for the Dm-myb mutant lethality. RNA interference (RNAi) to selectively remove each DmMyb complex member revealed that DmMyb protein levels are dependent upon the presence of several of the complex members. Together, these data support a model in which DmMyb activates a repressive complex containing Mip130 into a complex competent to support replication at specific loci in a temporally and developmentally proscribed manner.
[Keywords: Drosophila; DmMyb; replication; chorion amplification]
Received March 25, 2004; revised version accepted May 21, 2004.
Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1206604.
1 Corresponding author.
E-MAIL mbotchan{at}uclink.berkeley.edu; FAX (510) 643-1729.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Xie and T. L. Orr-Weaver Isolation of a Drosophila amplification origin developmentally activated by transcription PNAS, July 15, 2008; 105(28): 9651 - 9656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wen, L. Andrejka, J. Ashton, R. Karess, and J. S. Lipsick Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex Genes & Dev., March 1, 2008; 22(5): 601 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Georlette, S. Ahn, D. M. MacAlpine, E. Cheung, P. W. Lewis, E. L. Beall, S. P. Bell, T. Speed, J. R. Manak, and M. R. Botchan Genomic profiling and expression studies reveal both positive and negative activities for the Drosophila Myb MuvB/dREAM complex in proliferating cells Genes & Dev., November 15, 2007; 21(22): 2880 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Calvi, B. A. Byrnes, and A. J. Kolpakas Conservation of Epigenetic Regulation, ORC Binding and Developmental Timing of DNA Replication Origins in the Genus Drosophila Genetics, November 1, 2007; 177(3): 1291 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Park, D. M. MacAlpine, and T. L. Orr-Weaver Inaugural Article: Drosophila follicle cell amplicons as models for metazoan DNA replication: A cyclinE mutant exhibits increased replication fork elongation PNAS, October 23, 2007; 104(43): 16739 - 16746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Beall, P. W. Lewis, M. Bell, M. Rocha, D. L. Jones, and M. R. Botchan Discovery of tMAC: a Drosophila testis-specific meiotic arrest complex paralogous to Myb-Muv B Genes & Dev., April 15, 2007; 21(8): 904 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jiang, E. Benson, N. Bausek, K. Doggett, and H. White-Cooper Tombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with Always early Development, April 15, 2007; 134(8): 1549 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pilkinton, R. Sandoval, J. Song, S. A. Ness, and O. R. Colamonici Mip/LIN-9 Regulates the Expression of B-Myb and the Induction of Cyclin A, Cyclin B, and CDK1 J. Biol. Chem., January 5, 2007; 282(1): 168 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Harrison, C. J. Ceol, X. Lu, and H. R. Horvitz Some C. elegans class B synthetic multivulva proteins encode a conserved LIN-35 Rb-containing complex distinct from a NuRD-like complex PNAS, November 7, 2006; 103(45): 16782 - 16787. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Garcia and J. Frampton The transcription factor B-Myb is essential for S-phase progression and genomic stability in diploid and polyploid megakaryocytes J. Cell Sci., April 15, 2006; 119(8): 1483 - 1493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Morrison, J. S. Yakisich, D. Cassidy-Hanley, and G. M. Kapler TIF1 Represses rDNA Replication Initiation, but Promotes Normal S Phase Progression and Chromosome Transmission in Tetrahymena Mol. Biol. Cell, June 1, 2005; 16(6): 2624 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Lipsick synMuv verite--Myb comes into focus Genes & Dev., December 1, 2004; 18(23): 2837 - 2844. [Full Text] [PDF]
|
|
|
|
|
|
P. W. Lewis, E. L. Beall, T. C. Fleischer, D. Georlette, A. J. Link, and M. R. Botchan Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex Genes & Dev., December 1, 2004; 18(23): 2929 - 2940. [Abstract] [Full Text] [PDF]
|
|
