.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
Departament de Genètica Molecular, Institut de Biologia Molecular de Barcelona, CID-CSIC, 08034 Barcelona, Spain
Jasmonates (JA) are important regulators of plant defense responses that activate expression of many wound-induced genes including the tomato proteinase inhibitor II (pin2) and leucine aminopeptidase (LAP) genes. Elements required for JA induction of the LAP gene are all present in the -317 to -78 proximal promoter region. Using yeast one-hybrid screening, we have identified the bHLH-leu zipper JAMYC2 and JAMYC10 proteins, specifically recognizing a T/G-box AACGTG motif in this promoter fragment. Mutation of the G-box element decreases JA-responsive LAP promoter expression. Expression of JAMYC2 and JAMYC10 is induced by JA, with a kinetics that precedes that of the LAP or pin2 transcripts. JAMYC overexpression enhanced JA-induced expression of these defense genes in potato, but did not result in constitutive transcript accumulation. Using footprinting assays, an additional protected element was identified, located directly adjacent to the T/G-box motif. Mutation of this element abolishes JA response, showing that recognition of this duplicated element is also required for gene expression. Knockout mutants in the AtMYC2 homolog gene of Arabidopsis are insensitive to JA and exhibit a decreased activation of the JA-responsive genes AtVSP and JR1. Activation of the PDF1.2 and b-CHI, ethylene/JA-responsive genes, is, however, increased in these mutants. These results show that the JAMYC/AtMYC2 transcription factors function as members of a MYC-based regulatory system conserved in dicotyledonous plants with a key role in JA-induced defense gene activation.
[Keywords: pin2; LAP; wounding; herbivore attack; JA cross-talk]
Received January 22, 2004; revised version accepted May 5, 2004.
1 Corresponding author. E-MAIL sprat{at}cnb.uam.es; FAX 34-91-5854506.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X.-X. Shangguan, B. Xu, Z.-X. Yu, L.-J. Wang, and X.-Y. Chen Promoter of a cotton fibre MYB gene functional in trichomes of Arabidopsis and glandular trichomes of tobacco J. Exp. Bot., August 18, 2008; (2008) ern204v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Nabity, J. A. Zavala, and E. H. Delucia Indirect Suppression of Photosynthesis on Individual Leaves by Arthropod Herbivory Ann. Bot., July 26, 2008; (2008) mcn127v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Katsir, A. L. Schilmiller, P. E. Staswick, S. Y. He, and G. A. Howe From the Cover: COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine PNAS, May 13, 2008; 105(19): 7100 - 7105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, Y.-M. Woo, S.-I. Ryu, Y.-D. Shin, W. T. Kim, K. Y. Park, I.-J. Lee, and G. An Further Characterization of a Rice AGL12 Group MADS-Box Gene, OsMADS26 Plant Physiology, May 1, 2008; 147(1): 156 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kazan and J. M. Manners Jasmonate Signaling: Toward an Integrated View Plant Physiology, April 1, 2008; 146(4): 1459 - 1468. [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. van den Burg, D. I. Tsitsigiannis, O. Rowland, J. Lo, G. Rallapalli, D. MacLean, F. L.W. Takken, and J. D.G. Jones The F-Box Protein ACRE189/ACIF1 Regulates Cell Death and Defense Responses Activated during Pathogen Recognition in Tobacco and Tomato PLANT CELL, March 1, 2008; 20(3): 697 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-i. Arimura, S. Kopke, M. Kunert, V. Volpe, A. David, P. Brand, P. Dabrowska, M. E. Maffei, and W. Boland Effects of Feeding Spodoptera littoralis on Lima Bean Leaves: IV. Diurnal and Nocturnal Damage Differentially Initiate Plant Volatile Emission Plant Physiology, March 1, 2008; 146(3): 965 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Palmieri, S. Sell, X. Huang, M. Scherf, T. Werner, J. Durner, and C. Lindermayr Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: a bioinformatics approach J. Exp. Bot., February 13, 2008; (2008) erm345v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Bouchez, C. Huard, S. Lorrain, D. Roby, and C. Balague Ethylene Is One of the Key Elements for Cell Death and Defense Response Control in the Arabidopsis Lesion Mimic Mutant vad1 Plant Physiology, October 1, 2007; 145(2): 465 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhai, C.-B. Li, W. Zheng, X. Wu, J. Zhao, G. Zhou, H. Jiang, J. Sun, Y. Lou, and C. Li Phytochrome Chromophore Deficiency Leads to Overproduction of Jasmonic Acid and Elevated Expression of Jasmonate-Responsive Genes in Arabidopsis Plant Cell Physiol., July 1, 2007; 48(7): 1061 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Dombrecht, G. P. Xue, S. J. Sprague, J. A. Kirkegaard, J. J. Ross, J. B. Reid, G. P. Fitt, N. Sewelam, P. M. Schenk, J. M. Manners, et al. MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis PLANT CELL, July 1, 2007; 19(7): 2225 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vom Endt, M. Soares e Silva, J. W. Kijne, G. Pasquali, and J. Memelink Identification of a Bipartite Jasmonate-Responsive Promoter Element in the Catharanthus roseus ORCA3 Transcription Factor Gene That Interacts Specifically with AT-Hook DNA-Binding Proteins Plant Physiology, July 1, 2007; 144(3): 1680 - 1689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A.T. Adie, J. Perez-Perez, M. M. Perez-Perez, M. Godoy, J.-J. Sanchez-Serrano, E. A. Schmelz, and R. Solano ABA Is an Essential Signal for Plant Resistance to Pathogens Affecting JA Biosynthesis and the Activation of Defenses in Arabidopsis PLANT CELL, May 1, 2007; 19(5): 1665 - 1681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Takahashi, R. Yoshida, K. Ichimura, T. Mizoguchi, S. Seo, M. Yonezawa, K. Maruyama, K. Yamaguchi-Shinozaki, and K. Shinozaki The Mitogen-Activated Protein Kinase Cascade MKK3-MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in Arabidopsis PLANT CELL, March 1, 2007; 19(3): 805 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mallappa, V. Yadav, P. Negi, and S. Chattopadhyay A Basic Leucine Zipper Transcription Factor, G-box-binding Factor 1, Regulates Blue Light-mediated Photomorphogenic Growth in Arabidopsis J. Biol. Chem., August 4, 2006; 281(31): 22190 - 22199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zheng, Q. Zhai, J. Sun, C.-B. Li, L. Zhang, H. Li, X. Zhang, S. Li, Y. Xu, H. Jiang, et al. Bestatin, an Inhibitor of Aminopeptidases, Provides a Chemical Genetics Approach to Dissect Jasmonate Signaling in Arabidopsis Plant Physiology, August 1, 2006; 141(4): 1400 - 1413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, J. Zhao, H. Jiang, X. Wu, J. Sun, C. Zhang, X. Wang, Y. Lou, and C. Li The Wound Response Mutant suppressor of prosystemin-mediated responses6 (spr6) is a Weak Allele of the Tomato Homolog of CORONATINE-INSENSITIVE1 (COI1) Plant Cell Physiol., May 1, 2006; 47(5): 653 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Liechti, A. Gfeller, and E. E. Farmer Jasmonate Signaling Pathway Sci. Signal., February 14, 2006; 2006(322): cm2 - cm2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aksamit, A. Korobczak, J. Skala, M. Lukaszewicz, and J. Szopa The 14-3-3 Gene Expression Specificity in Response to Stress is Promoter-Dependent Plant Cell Physiol., October 1, 2005; 46(10): 1635 - 1645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. O. Steffens, C. Galuschka, M. Schindler, L. Bulow, and R. Hehl AthaMap web tools for database-assisted identification of combinatorial cis-regulatory elements and the display of highly conserved transcription factor binding sites in Arabidopsis thaliana Nucleic Acids Res., July 1, 2005; 33(suppl_2): W397 - W402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Yadav, C. Mallappa, S. N. Gangappa, S. Bhatia, and S. Chattopadhyay A Basic Helix-Loop-Helix Transcription Factor in Arabidopsis, MYC2, Acts as a Repressor of Blue Light-Mediated Photomorphogenic Growth PLANT CELL, July 1, 2005; 17(7): 1953 - 1966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Anderson, E. Badruzsaufari, P. M. Schenk, J. M. Manners, O. J. Desmond, C. Ehlert, D. J. Maclean, P. R. Ebert, and K. Kazan Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis PLANT CELL, December 1, 2004; 16(12): 3460 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gfeller and E. E. Farmer Keeping the Leaves Green Above Us Science, November 26, 2004; 306(5701): 1515 - 1516. [Abstract] [Full Text] [PDF]
|
|
