![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
Genome Damage and Stability Centre, University of Sussex, Brighton, BN1 9RQ, UK
To gain insight into the function and organization of proteins assembled on the DNA in response to genotoxic insult we investigated the phosphorylation of the Schizosaccharomyces pombe PCNA-like checkpoint protein Rad9. C-terminal T412/S423 phosphorylation of Rad9 by Rad3ATR occurs in S phase without replication stress. Rad3ATR and Tel1ATM phosphorylate these same residues, plus additional ones, in response to DNA damage. In S phase and after damage, only Rad9 phosphorylated on T412/S423, but not unphosphorylated Rad9, associates with a two-BRCT-domain region of the essential Rad4TOPBP1 protein. Rad9–Rad4TOPBP1 interaction is required to activate the Chk1 damage checkpoint but not the Cds1 replication checkpoint. When the Rad9-T412/S423 are phosphorylated, Rad4TOPBP1 coprecipitates with Rad3ATR, suggesting that phosphorylation coordinates formation of an active checkpoint complex.
[Keywords: Checkpoint; DNA replication; fission yeast; DNA damage; ATR]
Received November 7, 2003; revised version accepted March 29, 2004.
1 Present address: Pieris Proteolab AG, Lise-Meitner-Strasse 30, D-85354 Freising, Germany.
E-MAIL a.m.carr{at}sussex.ac.uk; FAX 44-1273-678121.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Puddu, M. Granata, L. Di Nola, A. Balestrini, G. Piergiovanni, F. Lazzaro, M. Giannattasio, P. Plevani, and M. Muzi-Falconi Phosphorylation of the Budding Yeast 9-1-1 Complex Is Required for Dpb11 Function in the Full Activation of the UV-Induced DNA Damage Checkpoint Mol. Cell. Biol., August 1, 2008; 28(15): 4782 - 4793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Burrows and S. J. Elledge How ATR turns on: TopBP1 goes on ATRIP with ATR Genes & Dev., June 1, 2008; 22(11): 1416 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Froget, J. Blaisonneau, S. Lambert, and G. Baldacci Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint Mol. Biol. Cell, February 1, 2008; 19(2): 445 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kanoh and M. Yanagida Tel2: a common partner of PIK-related kinases and a link between DNA checkpoint and nutritional response? Genes Cells, December 1, 2007; 12(12): 1301 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, A. Kumagai, and W. G. Dunphy The Rad9-Hus1-Rad1 Checkpoint Clamp Regulates Interaction of TopBP1 with ATR J. Biol. Chem., September 21, 2007; 282(38): 28036 - 28044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Delacroix, J. M. Wagner, M. Kobayashi, K.-i. Yamamoto, and L. M. Karnitz The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1 Genes & Dev., June 15, 2007; 21(12): 1472 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Ball, M. R. Ehrhardt, D. A. Mordes, G. G. Glick, W. J. Chazin, and D. Cortez Function of a Conserved Checkpoint Recruitment Domain in ATRIP Proteins Mol. Cell. Biol., May 1, 2007; 27(9): 3367 - 3377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zou Single- and double-stranded DNA: building a trigger of ATR-mediated DNA damage response Genes & Dev., April 15, 2007; 21(8): 879 - 885. [Full Text] [PDF]
|
|
|
|
|
|
L. Taricani and T. S. F. Wang Rad4TopBP1 Associates with Srr2, an Spc1 MAPK-regulated Protein, in Response to Environmental Stress J. Biol. Chem., March 23, 2007; 282(12): 8793 - 8800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Olson, C. J. Nievera, V. Klimovich, E. Fanning, and X. Wu RPA2 Is a Direct Downstream Target for ATR to Regulate the S-phase Checkpoint J. Biol. Chem., December 22, 2006; 281(51): 39517 - 39533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Liu, L. R. Barkley, T. Day, X. Bi, D. M. Slater, M. G. Alexandrow, H.-P. Nasheuer, and C. Vaziri The Chk1-mediated S-phase Checkpoint Targets Initiation Factor Cdc45 via a Cdc25A/Cdk2-independent Mechanism J. Biol. Chem., October 13, 2006; 281(41): 30631 - 30644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hashimoto, T. Tsujimura, A. Sugino, and H. Takisawa The phosphorylated C-terminal domain of Xenopus Cut5 directly mediates ATR-dependent activation of Chk1. Genes Cells, September 1, 2006; 11(9): 993 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Taricani and T. S.F. Wang Rad4TopBP1, a Scaffold Protein, Plays Separate Roles in DNA Damage and Replication Checkpoints and DNA Replication Mol. Biol. Cell, August 1, 2006; 17(8): 3456 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ogiwara, A. Ui, F. Onoda, S. Tada, T. Enomoto, and M. Seki Dpb11, the budding yeast homolog of TopBP1, functions with the checkpoint clamp in recombination repair Nucleic Acids Res., July 13, 2006; 34(11): 3389 - 3398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frampton, A. Irmisch, C. M. Green, A. Neiss, M. Trickey, H. D. Ulrich, K. Furuya, F. Z. Watts, A. M. Carr, and A. R. Lehmann Postreplication Repair and PCNA Modification in Schizosaccharomyces pombe Mol. Biol. Cell, July 1, 2006; 17(7): 2976 - 2985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-L. Du, T. M. Nakamura, and P. Russell Histone modification-dependent and -independent pathways for recruitment of checkpoint protein Crb2 to double-strand breaks. Genes & Dev., June 15, 2006; 20(12): 1583 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Lupardus and K. A. Cimprich Phosphorylation of Xenopus Rad1 and Hus1 Defines a Readout for ATR Activation That Is Independent of Claspin and the Rad9 Carboxy Terminus Mol. Biol. Cell, April 1, 2006; 17(4): 1559 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Nakamura, B. A. Moser, L.-L. Du, and P. Russell Cooperative Control of Crb2 by ATM Family and Cdc2 Kinases Is Essential for the DNA Damage Checkpoint in Fission Yeast Mol. Cell. Biol., December 15, 2005; 25(24): 10721 - 10730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Collura, J. Blaisonneau, G. Baldacci, and S. Francesconi The Fission Yeast Crb2/Chk1 Pathway Coordinates the DNA Damage and Spindle Checkpoint in Response to Replication Stress Induced by Topoisomerase I Inhibitor Mol. Cell. Biol., September 1, 2005; 25(17): 7889 - 7899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. O'Connell and K. A. Cimprich G2 damage checkpoints: what is the turn-on? J. Cell Sci., January 1, 2005; 118(1): 1 - 6. [Abstract] [Full Text] [PDF]
|
|
