![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Progesterone stimulation of Xenopus oocyte maturation requires the cytoplasmic polyadenylation-induced translation of mos and cyclin B mRNAs. One cis element that drives polyadenylation is the CPE, which is bound by the protein CPEB. Polyadenylation is stimulated by Aurora A (Eg2)-catalyzed CPEB serine 174 phosphorylation, which occurs soon after oocytes are exposed to progesterone. Here, we show that insulin also stimulates Aurora A-catalyzed CPEB S174 phosphorylation, cytoplasmic polyadenylation, translation, and oocyte maturation. However, these insulin-induced events are uniquely controlled by PI3 kinase and PKC-
, which act upstream of Aurora A. The intersection of the progesterone and insulin signaling pathways occurs at glycogen synthase kinase 3 (GSK-3), which regulates the activity of Aurora A. GSK-3 and Aurora A interact in vivo, and overexpressed GSK-3 inhibits Aurora A-catalyzed CPEB phosphorylation. In vitro, GSK-3 phosphorylates Aurora A on S290/291, the result of which is an autophosphorylation of serine 349. GSK-3 phosphorylated Aurora A, or Aurora A proteins with S290/291D or S349D mutations, have reduced or no capacity to phosphorylate CPEB. Conversely, Aurora A proteins with S290/291A or S349A mutations are constitutively active. These results suggest that the progesterone and insulin stimulate maturation by inhibiting GSK-3, which allows Aurora A activation and CPEB-mediated translation.
[Keywords: Insulin; GSK-3; Xenopus oocytes; Aurora A; cytoplasmic polyadenylation; CPEB]
Received July 23, 2003; revised version accepted November 19, 2003.
1 Present address: Center for Genomic Regulation, Passeig Maritim 37-49, 08003 Barcelona, Spain.
E-MAIL Joel.Richter{at}umassmed.edu; FAX (508) 856-4289.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Uzbekova, Y. Arlot-Bonnemains, J. Dupont, R. Dalbies-Tran, P. Papillier, S. Pennetier, A. Thelie, C. Perreau, P. Mermillod, C. Prigent, et al. Spatio-Temporal Expression Patterns of Aurora Kinases A, B, and C and Cytoplasmic Polyadenylation-Element-Binding Protein in Bovine Oocytes During Meiotic Maturation Biol Reprod, February 1, 2008; 78(2): 218 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Kim and J. D. Richter RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB Genes & Dev., October 15, 2007; 21(20): 2571 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Barr and F. Gergely Aurora-A: the maker and breaker of spindle poles J. Cell Sci., September 1, 2007; 120(17): 2987 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Keady, P. Kuo, S. E. Martinez, L. Yuan, and L. E. Hake MAPK interacts with XGef and is required for CPEB activation during meiosis in Xenopus oocytes J. Cell Sci., March 15, 2007; 120(6): 1093 - 1103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Racki and J. D. Richter CPEB controls oocyte growth and follicle development in the mouse Development, November 15, 2006; 133(22): 4527 - 4537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Salameh, J. P. Helliwell, G. Han, L. McPhaul, and O. Khorram Expression of endometrial glycogen synthase kinase-3{beta} protein throughout the menstrual cycle and its regulation by progesterone Mol. Hum. Reprod., September 1, 2006; 12(9): 543 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-Y. Jung, L. Lorenz, and J. D. Richter Translational Control by Neuroguidin, a Eukaryotic Initiation Factor 4E and CPEB Binding Protein. Mol. Cell. Biol., June 1, 2006; 26(11): 4277 - 4287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. Gadea and J. V. Ruderman Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin PNAS, March 21, 2006; 103(12): 4493 - 4498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Padmanabhan and J. D. Richter Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation Genes & Dev., January 15, 2006; 20(2): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Berger-Sweeney, N. R. Zearfoss, and J. D. Richter Reduced extinction of hippocampal-dependent memories in CPEB knockout mice Learn. Mem., January 1, 2006; 13(1): 4 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Cao, Y.-S. Huang, M.-C. Kan, and J. D. Richter Amyloid Precursor Proteins Anchor CPEB to Membranes and Promote Polyadenylation-Induced Translation Mol. Cell. Biol., December 15, 2005; 25(24): 10930 - 10939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Barnard, Q. Cao, and J. D. Richter Differential Phosphorylation Controls Maskin Association with Eukaryotic Translation Initiation Factor 4E and Localization on the Mitotic Apparatus Mol. Cell. Biol., September 1, 2005; 25(17): 7605 - 7615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Maton, T. Lorca, J.-A. Girault, R. Ozon, and C. Jessus Differential regulation of Cdc2 and Aurora-A in Xenopus oocytes: a crucial role of phosphatase 2A J. Cell Sci., June 1, 2005; 118(11): 2485 - 2494. [Abstract] [Full Text] [PDF]
|
|
