.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Biology, University of Konstanz, Germany.
Abstract
The rpoS-encoded sigma(S) subunit of RNA polymerase in Escherichia coli is a global regulatory factor involved in several stress responses. Mainly because of increased rpoS translation and stabilization of sigma(S), which in nonstressed cells is a highly unstable protein, the cellular sigma(S) content increases during entry into stationary phase and in response to hyperosmolarity. Here, we identify the hfq-encoded RNA-binding protein HF-I, which has been known previously only as a host factor for the replication of phage Qbeta RNA, as an essential factor for rpoS translation. An hfq null mutant exhibits strongly reduced sigma(S) levels under all conditions tested and is deficient for growth phase-related and osmotic induction of sigma(S). Using a combination of gene fusion analysis and pulse-chase experiments, we demonstrate that the hfq mutant is specifically impaired in rpoS translation. We also present evidence that the H-NS protein, which has been shown to affect rpoS translation, acts in the same regulatory pathway as HF-I at a position upstream of HF-I or in conjunction with HF-I. In addition, we show that expression and heat induction of the heat shock sigma factor sigma(32) (encoded by rpoH) is not dependent on HF-I, although rpoH and rpoS are both subject to translational regulation probably mediated by changes in mRNA secondary structure. HF-I is the first factor known to be specifically involved in rpoS translation, and this role is the first cellular function to be identified for this abundant ribosome-associated RNA-binding protein in E. coli.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. J. Soper and S. A. Woodson The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA RNA, September 1, 2008; 14(9): 1907 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Kulesus, K. Diaz-Perez, E. S. Slechta, D. S. Eto, and M. A. Mulvey Impact of the RNA Chaperone Hfq on the Fitness and Virulence Potential of Uropathogenic Escherichia coli Infect. Immun., July 1, 2008; 76(7): 3019 - 3026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Vecerek, L. Rajkowitsch, E. Sonnleitner, R. Schroeder, and U. Blasi The C-terminal domain of Escherichia coli Hfq is required for regulation Nucleic Acids Res., January 17, 2008; 36(1): 133 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Nielsen, A. Boggild, C. B.F. Andersen, G. Nielsen, A. Boysen, D. E. Brodersen, and P. Valentin-Hansen An Hfq-like protein in archaea: Crystal structure and functional characterization of the Sm protein from Methanococcus jannaschii RNA, December 1, 2007; 13(12): 2213 - 2223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Suzuki, T. Aono, K.-B. Lee, T. Suzuki, C.-T. Liu, H. Miwa, S. Wakao, T. Iki, and H. Oyaizu Rhizobial Factors Required for Stem Nodule Maturation and Maintenance in Sesbania rostrata-Azorhizobium caulinodans ORS571 Symbiosis Appl. Envir. Microbiol., October 15, 2007; 73(20): 6650 - 6659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Heidrich, I. Moll, and S. Brantl In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA Nucleic Acids Res., July 26, 2007; 35(13): 4331 - 4346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Jones, A. Goodwill, and T. Elliott Limited Role for the DsrA and RprA Regulatory RNAs in rpoS Regulation in Salmonella enterica. J. Bacteriol., July 1, 2006; 188(14): 5077 - 5088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ziolkowska, P. Derreumaux, M. Folichon, O. Pellegrini, P. Regnier, I. V. Boni, and E. Hajnsdorf Hfq variant with altered RNA binding functions Nucleic Acids Res., January 30, 2006; 34(2): 709 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Will and L. S. Frost Characterization of the Opposing Roles of H-NS and TraJ in Transcriptional Regulation of the F-Plasmid tra Operon J. Bacteriol., January 15, 2006; 188(2): 507 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Will and L. S. Frost Hfq Is a Regulator of F-Plasmid TraJ and TraM Synthesis in Escherichia coli J. Bacteriol., January 1, 2006; 188(1): 124 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Takada, K. Nagai, and M. Wachi A decreased level of FtsZ is responsible for inviability of RNase E-deficient cells Genes Cells, July 1, 2005; 10(7): 733 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. VECEREK, I. MOLL, and U. BLASI Translational autocontrol of the Escherichia coli hfq RNA chaperone gene RNA, June 1, 2005; 11(6): 976 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wang and D. E. Crowley Global Gene Expression Responses to Cadmium Toxicity in Escherichia coli J. Bacteriol., May 1, 2005; 187(9): 3259 - 3266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sevo, E. Buratti, and V. Venturi Ribosomal Protein S1 Specifically Binds to the 5' Untranslated Region of the Pseudomonas aeruginosa Stationary-Phase Sigma Factor rpoS mRNA in the Logarithmic Phase of Growth J. Bacteriol., August 1, 2004; 186(15): 4903 - 4909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dole, Y. Klingen, V. Nagarajavel, and K. Schnetz The Protease Lon and the RNA-Binding Protein Hfq Reduce Silencing of the Escherichia coli bgl Operon by H-NS J. Bacteriol., May 1, 2004; 186(9): 2708 - 2716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Karring, S. G. J. Mathu, J. van Duin, B. F. C. Clark, B. Kraal, and C. R. Knudsen Q{beta}-Phage Resistance by Deletion of the Coiled-coil Motif in Elongation Factor Ts J. Biol. Chem., January 16, 2004; 279(3): 1878 - 1884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Folichon, V. Arluison, O. Pellegrini, E. Huntzinger, P. Regnier, and E. Hajnsdorf The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation Nucleic Acids Res., December 15, 2003; 31(24): 7302 - 7310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ruiz and T. J. Silhavy Constitutive Activation of the Escherichia coli Pho Regulon Upregulates rpoS Translation in an Hfq-Dependent Fashion J. Bacteriol., October 15, 2003; 185(20): 5984 - 5992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sauter, J. Basquin, and D. Suck Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli Nucleic Acids Res., July 15, 2003; 31(14): 4091 - 4098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Worhunsky, K. Godek, S. Litsch, and P. J. Schlax Interactions of the Non-coding RNA DsrA and RpoS mRNA with the 30 S Ribosomal Subunit J. Biol. Chem., April 25, 2003; 278(18): 15815 - 15824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ko and G. A. Splitter Molecular Host-Pathogen Interaction in Brucellosis: Current Understanding and Future Approaches to Vaccine Development for Mice and Humans Clin. Microbiol. Rev., January 1, 2003; 16(1): 65 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Madrid, J. M. Nieto, S. Paytubi, M. Falconi, C. O. Gualerzi, and A. Juarez Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin J. Bacteriol., September 15, 2002; 184(18): 5058 - 5066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hirsch and T. Elliott Role of ppGpp in rpoS Stationary-Phase Regulation in Escherichia coli J. Bacteriol., September 15, 2002; 184(18): 5077 - 5087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hengge-Aronis Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase Microbiol. Mol. Biol. Rev., September 1, 2002; 66(3): 373 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Sun, I. Zhulin, and R. M. Wartell Predicted structure and phyletic distribution of the RNA-binding protein Hfq Nucleic Acids Res., September 1, 2002; 30(17): 3662 - 3671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Brown, D. Gentry, T. Elliott, and M. Cashel DksA Affects ppGpp Induction of RpoS at a Translational Level J. Bacteriol., August 15, 2002; 184(16): 4455 - 4465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Rajkumari and J. Gowrishankar An N-Terminally Truncated RpoS ({sigma}S) Protein in Escherichia coli Is Active In Vivo and Exhibits Normal Environmental Regulation Even in the Absence of rpoS Transcriptional and Translational Control Signals J. Bacteriol., June 15, 2002; 184(12): 3167 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sonnleitner, I. Moll, and U. Blasi Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa Microbiology, March 1, 2002; 148(3): 883 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Nieto, C. Madrid, E. Miquelay, J. L. Parra, S. Rodriguez, and A. Juarez Evidence for Direct Protein-Protein Interaction between Members of the Enterobacterial Hha/YmoA and H-NS Families of Proteins J. Bacteriol., February 1, 2002; 184(3): 629 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Rajkumari and J. Gowrishankar In Vivo Expression from the RpoS-Dependent P1 Promoter of the Osmotically Regulated proU Operon in Escherichia coli and Salmonella enterica Serovar Typhimurium: Activation by rho and hns Mutations and by Cold Stress J. Bacteriol., November 15, 2001; 183(22): 6543 - 6550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Seshadri and J. E. Samuel Characterization of a Stress-Induced Alternate Sigma Factor, RpoS, of Coxiella burnetii and Its Expression during the Development Cycle Infect. Immun., August 1, 2001; 69(8): 4874 - 4883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Wassarman, F. Repoila, C. Rosenow, G. Storz, and S. Gottesman Identification of novel small RNAs using comparative genomics and microarrays Genes & Dev., July 1, 2001; 15(13): 1637 - 1651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Sledjeski, C. Whitman, and A. Zhang Hfq Is Necessary for Regulation by the Untranslated RNA DsrA J. Bacteriol., March 15, 2001; 183(6): 1997 - 2005. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Bidle and D. H. Bartlett RNA Arbitrarily Primed PCR Survey of Genes Regulated by ToxR in the Deep-Sea Bacterium Photobacterium profundum Strain SS9 J. Bacteriol., March 1, 2001; 183(5): 1688 - 1693. [Abstract] [Full Text]
|
|
|
|
|
|
C. Ueguchi, N. Misonou, and T. Mizuno Negative Control of rpoS Expression by Phosphoenolpyruvate:Carbohydrate Phosphotransferase System in Escherichia coli J. Bacteriol., January 15, 2001; 183(2): 520 - 527. [Abstract] [Full Text]
|
|
|
|
 |
|
 X. Nou and R. J. Kadner Adenosylcobalamin inhibits ribosome binding to btuB RNA PNAS, June 13, 2000; (2000) 130013897. [Abstract] [Full Text]
|
|
|
|
|
|
O. Vytvytska, I. Moll, V. R. Kaberdin, A. von Gabain, and U. Bläsi Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding Genes & Dev., May 1, 2000; 14(9): 1109 - 1118. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. Azam and A. Ishihama Twelve Species of the Nucleoid-associated Protein from Escherichia coli. SEQUENCE RECOGNITION SPECIFICITY AND DNA BINDING AFFINITY J. Biol. Chem., November 12, 1999; 274(46): 33105 - 33113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ali Azam, A. Iwata, A. Nishimura, S. Ueda, and A. Ishihama Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid J. Bacteriol., October 15, 1999; 181(20): 6361 - 6370. [Abstract] [Full Text]
|
|
|
|
|
|
G. Becker, E. Klauck, and R. Hengge-Aronis Regulation of RpoS proteolysis in Escherichia coli: The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS PNAS, May 25, 1999; 96(11): 6439 - 6444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Paesold and M. Krause Analysis of rpoS mRNA in Salmonella dublin: Identification of Multiple Transcripts with Growth-Phase-Dependent Variation in Transcript Stability J. Bacteriol., February 15, 1999; 181(4): 1264 - 1268. [Abstract] [Full Text]
|
|
|
|
|
|
X. Nou and R. J. Kadner Coupled Changes in Translation and Transcription during Cobalamin-Dependent Regulation of btuB Expression in Escherichia coli J. Bacteriol., December 15, 1998; 180(24): 6719 - 6728. [Abstract] [Full Text]
|
|
|
|
|
|
O. Vytvytska, J. S. Jakobsen, G. Balcunaite, J. S. Andersen, M. Baccarini, and A. von Gabain Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability PNAS, November 24, 1998; 95(24): 14118 - 14123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Lease, M. E. Cusick, and M. Belfort Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci PNAS, October 13, 1998; 95(21): 12456 - 12461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Majdalani, C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription PNAS, October 13, 1998; 95(21): 12462 - 12467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cunning, L. Brown, and T. Elliott Promoter Substitution and Deletion Analysis of Upstream Region Required for rpoS Translational Regulation J. Bacteriol., September 1, 1998; 180(17): 4564 - 4570. [Abstract] [Full Text]
|
|
|
|
|
|
D. Schuppli, G. Miranda, H.-C. T. Tsui, M. E. Winkler, J. M. Sogo, and H. Weber Altered 3'-terminal RNA structure in phage Qbeta adapted to host factor-less Escherichia coli PNAS, September 16, 1997; 94(19): 10239 - 10242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hajnsdorf and P. Regnier Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I PNAS, February 15, 2000; 97(4): 1501 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Nou and R. J. Kadner Adenosylcobalamin inhibits ribosome binding to btuB RNA PNAS, June 20, 2000; 97(13): 7190 - 7195. [Abstract] [Full Text] [PDF]
|
|
