![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
Abstract
Mice deficient for the RB gene (RB-/-), prior to death at embryonic day 14.5, show increased cell death in all tissues that normally express RB1: the nervous system, liver, lens, and skeletal muscle precursor cells. We have generated transgenic mice (RBlox) that express low levels of pRb, driven by an RB1 minigene. RBlox/RB-/- mutant fetuses die at birth with specific skeletal muscle defects, including increased cell death prior to myoblast fusion, shorter myotubes with fewer myofibrils, reduced muscle fibers, accumulation of elongated nuclei that actively synthesized DNA within the myotubes, and reduction in expression of the late muscle-specific genes MCK and MRF4. Thus, insufficient pRb results in failure of myogenesis in vivo, manifest in two ways. First, the massive apoptosis of myoblasts implicates a role of pRb in cell survival. Second, surviving myotubes failed to develop normally and accumulated large polyploid nuclei, implicating pRb in permanent withdrawal from the cell cycle. These results demonstrate a role for pRb during terminal differentiation of skeletal muscles in vivo and place pRb at a nodal point that controls cell proliferation, differentiation, and death.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. A. Mason-Richie, M. J. Mistry, C. A. Gettler, A. Elayyadi, and K. A. Wikenheiser-Brokamp Retinoblastoma Function Is Essential for Establishing Lung Epithelial Quiescence after Injury Cancer Res., June 1, 2008; 68(11): 4068 - 4076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Weber, M. K. Corbett, L. M. L. Chow, M. B. Valentine, S. J. Baker, and J. Zuo Rapid cell-cycle reentry and cell death after acute inactivation of the retinoblastoma gene product in postnatal cochlear hair cells PNAS, January 15, 2008; 105(2): 781 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Blais, C. J.C. van Oevelen, R. Margueron, D. Acosta-Alvear, and B. D. Dynlacht Retinoblastoma tumor suppressor protein dependent methylation of histone H3 lysine 27 is associated with irreversible cell cycle exit J. Cell Biol., December 31, 2007; 179(7): 1399 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Ho, Q. H. Li, H. Okada, T. W. Mak, and E. Zacksenhaus XIAP Activity Dictates Apaf-1 Dependency for Caspase 9 Activation Mol. Cell. Biol., August 15, 2007; 27(16): 5673 - 5685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Luan, X.-P. Yu, K. Xu, B. Ding, J. Yu, Y. Huang, N. Yang, P. Lengyel, P. E. Di Cesare, and C.-j. Liu The Retinoblastoma Protein Is an Essential Mediator of Osteogenesis That Links the p204 Protein to the Cbfa1 Transcription Factor Thereby Increasing Its Activity J. Biol. Chem., June 8, 2007; 282(23): 16860 - 16870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi and M. E. Ewen Genetic Interaction between Rb and N-ras: Differentiation Control and Metastasis Cancer Res., October 1, 2006; 66(19): 9345 - 9348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Nitta, S. A. Jameson, B. A. Kudlow, L. A. Conlan, and B. K. Kennedy Stabilization of the Retinoblastoma Protein by A-Type Nuclear Lamins Is Required for INK4A-Mediated Cell Cycle Arrest. Mol. Cell. Biol., July 1, 2006; 26(14): 5360 - 5372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Saab, J. L. Bills, A. P. Miceli, C. M. Anderson, J. D. Khoury, D. W. Fry, F. Navid, P. J. Houghton, and S. X. Skapek Pharmacologic inhibition of cyclin-dependent kinase 4/6 activity arrests proliferation in myoblasts and rhabdomyosarcoma-derived cells Mol. Cancer Ther., May 1, 2006; 5(5): 1299 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. MacWilliams, K. Doquang, R. Pedrola, G. Dollman, D. Grassi, T. Peis, A. Tsang, and A. Ceccarelli A retinoblastoma ortholog controls stalk/spore preference in Dictyostelium Development, April 1, 2006; 133(7): 1287 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Melcon, S. Kozlov, D. A. Cutler, T. Sullivan, L. Hernandez, P. Zhao, S. Mitchell, G. Nader, M. Bakay, J. N. Rottman, et al. Loss of emerin at the nuclear envelope disrupts the Rb1/E2F and MyoD pathways during muscle regeneration Hum. Mol. Genet., February 15, 2006; 15(4): 637 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Frock, B. A. Kudlow, A. M. Evans, S. A. Jameson, S. D. Hauschka, and B. K. Kennedy Lamin A/C and emerin are critical for skeletal muscle satellite cell differentiation. Genes & Dev., February 15, 2006; 20(4): 486 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Collins, T. Nakahara, A. Do, and P. F. Lambert Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle J. Virol., December 1, 2005; 79(23): 14769 - 14780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Delehouzee, T. Yoshikawa, C. Sawa, J.-i. Sawada, T. Ito, M. Omori, T. Wada, Y. Yamaguchi, Y. Kabe, and H. Handa GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis Genes Cells, July 1, 2005; 10(7): 717 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. K. Clay and T. Nelson The Recessive Epigenetic swellmap Mutation Affects the Expression of Two Step II Splicing Factors Required for the Transcription of the Cell Proliferation Gene STRUWWELPETER and for the Timing of Cell Cycle Arrest in the Arabidopsis Leaf PLANT CELL, July 1, 2005; 17(7): 1994 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mantela, Z. Jiang, J. Ylikoski, B. Fritzsch, E. Zacksenhaus, and U. Pirvola The retinoblastoma gene pathway regulates the postmitotic state of hair cells of the mouse inner ear Development, May 15, 2005; 132(10): 2377 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wells, M.G. Bermudez, N. Steuerwald, A.R. Thornhill, D.L. Walker, H. Malter, J.D.A. Delhanty, and J. Cohen Expression of genes regulating chromosome segregation, the cell cycle and apoptosis during human preimplantation development Hum. Reprod., May 1, 2005; 20(5): 1339 - 1348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Dorval, B. P. Bobechko, K. F. Ahmad, and R. Bremner Transcriptional Activity of the Paired-like Homeodomain Proteins CHX10 and VSX1 J. Biol. Chem., March 18, 2005; 280(11): 10100 - 10108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-j. Liu, E. Chang, J. Yu, C. S. Carlson, L. Prazak, X.-P. Yu, B. Ding, P. Lengyel, and P. E. D. Cesare The Interferon-inducible p204 Protein Acts as a Transcriptional Coactivator of Cbfa1 and Enhances Osteoblast Differentiation J. Biol. Chem., January 28, 2005; 280(4): 2788 - 2796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. St-Pierre, X. Liu, L.-C. T. Kha, X. Zhu, O. Ryan, Z. Jiang, and E. Zacksenhaus Conserved and specific functions of mammalian ssu72 Nucleic Acids Res., January 19, 2005; 33(2): 464 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Markiewicz, M. Ledran, and C. J. Hutchison Remodelling of the nuclear lamina and nucleoskeleton is required for skeletal muscle differentiation in vitro J. Cell Sci., January 15, 2005; 118(2): 409 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi, B. Contreras, R. T. Bronson, M. Loda, and M. E. Ewen Genetic Interaction between Rb and K-ras in the Control of Differentiation and Tumor Suppression Mol. Cell. Biol., December 1, 2004; 24(23): 10406 - 10415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Camarda, F. Siepi, D. Pajalunga, C. Bernardini, R. Rossi, A. Montecucco, E. Meccia, and M. Crescenzi A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells J. Cell Biol., November 8, 2004; 167(3): 417 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Huh, M. H. Parker, A. Scime, R. Parks, and M. A. Rudnicki Rb is required for progression through myogenic differentiation but not maintenance of terminal differentiation J. Cell Biol., September 13, 2004; 166(6): 865 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ishido, K. Kami, and M. Masuhara In vivo expression patterns of MyoD, p21, and Rb proteins in myonuclei and satellite cells of denervated rat skeletal muscle Am J Physiol Cell Physiol, August 1, 2004; 287(2): C484 - C493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacPherson, J. Sage, T. Kim, D. Ho, M. E. McLaughlin, and T. Jacks Cell type-specific effects of Rb deletion in the murine retina Genes & Dev., July 15, 2004; 18(14): 1681 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Johnson, R. T. Nitta, R. L. Frock, L. Mounkes, D. A. Barbie, C. L. Stewart, E. Harlow, and B. K. Kennedy A-type lamins regulate retinoblastoma protein function by promoting subnuclear localization and preventing proteasomal degradation PNAS, June 29, 2004; 101(26): 9677 - 9682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Fletcher, D. Easton, K. Anderson, C. Gilham, M. Jay, and J. Peto Lifetime Risks of Common Cancers Among Retinoblastoma Survivors J Natl Cancer Inst, March 3, 2004; 96(5): 357 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Favreau, D. Higuet, J.-C. Courvalin, and B. Buendia Expression of a Mutant Lamin A That Causes Emery-Dreifuss Muscular Dystrophy Inhibits In Vitro Differentiation of C2C12 Myoblasts Mol. Cell. Biol., February 15, 2004; 24(4): 1481 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi, R. T. Bronson, M. Socolovsky, B. Contreras, K. Y. Lee, T. Jacks, M. Noda, R. Kucherlapati, and M. E. Ewen Rb and N-ras Function Together To Control Differentiation in the Mouse Mol. Cell. Biol., August 1, 2003; 23(15): 5256 - 5268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Ostrovsky and E. Bengal The Mitogen-activated Protein Kinase Cascade Promotes Myoblast Cell Survival by Stabilizing the Cyclin-dependent Kinase Inhibitor, p21WAF1 Protein J. Biol. Chem., May 30, 2003; 278(23): 21221 - 21231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Bruin, L. Wu, H. I. Saavedra, P. Wilson, Y. Yang, T. J. Rosol, M. Weinstein, M. L. Robinson, and G. Leone Rb function in extraembryonic lineages suppresses apoptosis in the CNS of Rb-deficient mice PNAS, May 27, 2003; 100(11): 6546 - 6551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Magenta, C. Cenciarelli, F. De Santa, P. Fuschi, F. Martelli, M. Caruso, and A. Felsani MyoD Stimulates RB Promoter Activity via the CREB/p300 Nuclear Transduction Pathway Mol. Cell. Biol., April 15, 2003; 23(8): 2893 - 2906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. von Willebrand, E. Zacksenhaus, E. Cheng, P. Glazer, and R. Halaban The Tyrphostin AG1024 Accelerates the Degradation of Phosphorylated Forms of Retinoblastoma Protein (pRb) and Restores pRb Tumor Suppressive Function in Melanoma Cells Cancer Res., March 15, 2003; 63(6): 1420 - 1429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xia, A. Cheng, D. Akinmade, and A. W. Hamburger The N-Terminal 24 Amino Acids of the p55 Gamma Regulatory Subunit of Phosphoinositide 3-Kinase Binds Rb and Induces Cell Cycle Arrest Mol. Cell. Biol., March 1, 2003; 23(5): 1717 - 1725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacPherson, J. Sage, D. Crowley, A. Trumpp, R. T. Bronson, and T. Jacks Conditional Mutation of Rb Causes Cell Cycle Defects without Apoptosis in the Central Nervous System Mol. Cell. Biol., February 1, 2003; 23(3): 1044 - 1053. [Abstract] [Full Text]
|
|
|
|
|
|
W. Dewitte, C. Riou-Khamlichi, S. Scofield, J. M. S. Healy, A. Jacqmard, N. J. Kilby, and J. A. H. Murray Altered Cell Cycle Distribution, Hyperplasia, and Inhibited Differentiation in Arabidopsis Caused by the D-Type Cyclin CYCD3 PLANT CELL, January 1, 2003; 15(1): 79 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Guo, S. Yikang, H. Yoshida, T. W. Mak, and E. Zacksenhaus Inactivation of the Retinoblastoma Tumor Suppressor Induces Apoptosis Protease-activating Factor-1 Dependent and Independent Apoptotic Pathways during Embryogenesis Cancer Res., December 1, 2001; 61(23): 8395 - 8400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Shih, M. Xiu, S. P. Berasi, E. M. Sampson, A. Leiter, K. E. Paulson, and A. S. Yee HMG Box Transcriptional Repressor HBP1 Maintains a Proliferation Barrier in Differentiated Liver Tissue Mol. Cell. Biol., September 1, 2001; 21(17): 5723 - 5732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yu. Nikitin, B. Shan, A. Flesken-Nikitin, K.-H. Chang, and W.-H. Lee The Retinoblastoma Gene Regulates Somatic Growth during Mouse Development Cancer Res., April 1, 2001; 61(7): 3110 - 3118. [Abstract] [Full Text]
|
|
|
|
|
|
A. Divan, J. Lawry, I. R. Dunsmore, M. A. Parsons, and J. A. Royds p53 and p21waf-1 Expression Correlates with Apoptosis or Cell Survival in Poorly Differentiated, but not Well-Differentiated, Retinoblastomas Cancer Res., April 1, 2001; 61(7): 3157 - 3163. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Hirsch, L. Gu, and R. W. Henry The Retinoblastoma Tumor Suppressor Protein Targets Distinct General Transcription Factors To Regulate RNA Polymerase III Gene Expression Mol. Cell. Biol., December 15, 2000; 20(24): 9182 - 9191. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Lawlor and P. Rotwein Coordinate Control of Muscle Cell Survival by Distinct Insulin-like Growth Factor Activated Signaling Pathways J. Cell Biol., December 4, 2000; 151(6): 1131 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miyake, W. R. Sellers, M. Safran, X. Li, W. Zhao, S. R. Grossman, J. Gan, J. A. DeCaprio, P. D. Adams, and W. G. Kaelin Jr. Cells Degrade a Novel Inhibitor of Differentiation with E1A-Like Properties upon Exiting the Cell Cycle Mol. Cell. Biol., December 1, 2000; 20(23): 8889 - 8902. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Wang, S. W. Hayward, A. A. Donjacour, P. Young, T. Jacks, J. Sage, R. Dahiya, R. D. Cardiff, M. L. Day, and G. R. Cunha Sex Hormone-induced Carcinogenesis in Rb-deficient Prostate Tissue Cancer Res., November 1, 2000; 60(21): 6008 - 6017. [Abstract] [Full Text]
|
|
|
|
|
|
M. Classon, B. K. Kennedy, R. Mulloy, and E. Harlow Opposing roles of pRB and p107 in adipocyte differentiation PNAS, September 19, 2000; (2000) 190343597. [Abstract] [Full Text]
|
|
|
|
|
|
T.-T. Chen and J. Y. J. Wang Establishment of Irreversible Growth Arrest in Myogenic Differentiation Requires the RB LXCXE-Binding Function Mol. Cell. Biol., August 1, 2000; 20(15): 5571 - 5580. [Abstract] [Full Text]
|
|
|
|
|
|
F.-Q. Li, A. Coonrod, and M. Horwitz Selection of a Dominant Negative Retinoblastoma Protein (RB) Inhibiting Satellite Myoblast Differentiation Implies an Indirect Interaction between MyoD and RB Mol. Cell. Biol., July 15, 2000; 20(14): 5129 - 5139. [Abstract] [Full Text]
|
|
|
|
|
|
L. Vitelli, G. Condorelli, V. Lulli, T. Hoang, L. Luchetti, C. M. Croce, and C. Peschle A Pentamer Transcriptional Complex Including tal-1 and Retinoblastoma Protein Downmodulates c-kit Expression in Normal Erythroblasts Mol. Cell. Biol., July 15, 2000; 20(14): 5330 - 5342. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Sheng, T. M. Love, and B. Schaffhausen J Domain-Independent Regulation of the Rb Family by Polyomavirus Large T Antigen J. Virol., June 1, 2000; 74(11): 5280 - 5290. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Chen, D. H. Dowhan, B. M. Hosking, and G. E.O. Muscat The steroid receptor coactivator, GRIP-1, is necessary for MEF-2C-dependent gene expression and skeletal muscle differentiation Genes & Dev., May 15, 2000; 14(10): 1209 - 1228. [Abstract] [Full Text]
|
|
|
|
|
|
A. Mal, D. Chattopadhyay, M. K. Ghosh, R. Y.C. Poon, T. Hunter, and M. L. Harter p21 and Retinoblastoma Protein Control the Absence of DNA Replication in Terminally Differentiated Muscle Cells J. Cell Biol., April 17, 2000; 149(2): 281 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gill and P. A. Hamel Subcellular Compartmentalization of E2F Family Members Is Required for Maintenance of the Postmitotic State in Terminally Differentiated Muscle J. Cell Biol., March 20, 2000; 148(6): 1187 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. X. Skapek, D. Jansen, T.-F. Wei, T. McDermott, W. Huang, E. N. Olson, and E. Y.-H. P. Lee Cloning and Characterization of a Novel Kruppel-associated Box Family Transcriptional Repressor That Interacts with the Retinoblastoma Gene Product, RB J. Biol. Chem., March 15, 2000; 275(10): 7212 - 7223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Guardavaccaro, G. Corrente, F. Covone, L. Micheli, I. D'Agnano, G. Starace, M. Caruso, and F. Tirone Arrest of G1-S Progression by the p53-Inducible Gene PC3 Is Rb Dependent and Relies on the Inhibition of Cyclin D1 Transcription Mol. Cell. Biol., March 1, 2000; 20(5): 1797 - 1815. [Abstract] [Full Text]
|
|
|
|
|
|
W Du Suppression of the rbf null mutants by a de2f1 allele that lacks transactivation domain Development, January 1, 2000; 127(2): 367 - 379. [Abstract] [PDF]
|
|
|
|
|
|
K. Y. Lee, M. H. Ladha, C. McMahon, and M. E. Ewen The Retinoblastoma Protein Is Linked to the Activation of Ras Mol. Cell. Biol., November 1, 1999; 19(11): 7724 - 7732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Doostzadeh-Cizeron, R. Evans, S. Yin, and D. W. Goodrich Apoptosis Induced by the Nuclear Death Domain Protein p84N5 Is Inhibited by Association with Rb Protein Mol. Biol. Cell, October 1, 1999; 10(10): 3251 - 3261. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Hu, P. J. Cascone, L. Cheng, D. Sun, J. R. Nambu, and L. M. Schwartz Lepidopteran DALP, and its mammalian ortholog HIC-5, function as negative regulators of muscle differentiation PNAS, August 31, 1999; 96(18): 10218 - 10223. [Abstract] [Full Text] [PDF]
|
|
