QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tracy, R B Articles by Kowalczykowski, S C Search for Related Content PubMed PubMed Citation Articles by Tracy, R B Articles by Kowalczykowski, S C Social Bookmarking                   What's this?

Research Papers

In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein.

Division of Biological Sciences, University of California at Davis 95616, USA.

Abstract

The RecA protein and other DNA strand exchange proteins are characterized by their ability to bind and pair DNA in a sequence-independent manner. In vitro selection experiments demonstrate, unexpectedly, that RecA protein has a preferential affinity for DNA sequences rich in GT composition. Such GT-rich sequences are present in loci that display increased recombinational activity in both eukaryotes and prokaryotes, including the Escherichia coli recombination hotspot, chi (5'-GCTGGTGG-3'). Interestingly, these selected sequences, or chi-containing substrates, display both an enhanced rate and extent of homologous pairing in RecA protein-dependent homologous pairing reactions. Thus, the binding and pairing of DNA by RecA protein is composition-dependent, suggesting that a component of the elevated recombinational activity of chi and increased genomic rearrangements at certain DNA sequences in eukaryotes is contributed by enhanced DNA pairing activity.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				E. M. Seitz and S. C. Kowalczykowski Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci Nucleic Acids Res., May 24, 2006; 34(10): 2847 - 2852. [Abstract] [Full Text] [PDF]

				R. Rajan, J. W. Wisler, and C. E. Bell Probing the DNA sequence specificity of Escherichia coli RECA protein. Nucleic Acids Res., January 1, 2006; 34(8): 2463 - 2471. [Abstract] [Full Text] [PDF]

				L. S. Symington Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 630 - 670. [Abstract] [Full Text] [PDF]

				V. F. Holmes, F. Scandellari, K. R. Benjamin, and N. R. Cozzarelli Structure of Reaction Intermediates Formed during Saccharomyces cerevisiae Rad51-catalyzed Strand Transfer J. Biol. Chem., October 4, 2002; 277(41): 38945 - 38953. [Abstract] [Full Text] [PDF]

				M. Prudhomme, V. Libante, and J.-P. Claverys Homologous recombination at the border: Insertion-deletions and the trapping of foreign DNA in Streptococcus pneumoniae PNAS, February 19, 2002; 99(4): 2100 - 2105. [Abstract] [Full Text] [PDF]

				R. Bar-Ziv and A. Libchaber Effects of DNA sequence and structure on binding of RecA to single-stranded DNA PNAS, July 19, 2001; (2001) 151242898. [Abstract] [Full Text] [PDF]

				X. Pan and D. R. F. Leach The roles of mutS, sbcCD and recA in the propagation of TGG repeats in Escherichia coli Nucleic Acids Res., August 15, 2000; 28(16): 3178 - 3184. [Abstract] [Full Text] [PDF]

				D. A. Arnold and S. C. Kowalczykowski Facilitated Loading of RecA Protein Is Essential to Recombination by RecBCD Enzyme J. Biol. Chem., April 14, 2000; 275(16): 12261 - 12265. [Abstract] [Full Text] [PDF]

				K. Muniyappa, S. Anuradha, and B. Byers Yeast Meiosis-Specific Protein Hop1 Binds to G4 DNA and Promotes Its Formation Mol. Cell. Biol., February 15, 2000; 20(4): 1361 - 1369. [Abstract] [Full Text]

				E. M. Seitz, J. P. Brockman, S. J. Sandler, A. J. Clark, and S. C. Kowalczykowski RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange Genes & Dev., May 1, 1998; 12(9): 1248 - 1253. [Abstract] [Full Text]

				R. B. Tracy, J. K. Baumohl, and S. C. Kowalczykowski The preference for GT-rich DNA by the yeast Rad51 protein defines a set of universal pairing sequences Genes & Dev., December 15, 1997; 11(24): 3423 - 3431. [Abstract] [Full Text] [PDF]

				D G Anderson and S C Kowalczykowski The recombination hot spot chi is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme. Genes & Dev., March 1, 1997; 11(5): 571 - 581. [Abstract] [PDF]

				A. A. Volodin and R. D. Camerini-Otero Influence of DNA Sequence on the Positioning of RecA Monomers in RecA-DNA Cofilaments J. Biol. Chem., January 4, 2002; 277(2): 1614 - 1618. [Abstract] [Full Text] [PDF]

				R. Bar-Ziv and A. Libchaber Effects of DNA sequence and structure on binding of RecA to single-stranded DNA PNAS, July 31, 2001; 98(16): 9068 - 9073. [Abstract] [Full Text] [PDF]